Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   FORC23_RS03245 Genome accession   NZ_CP012950
Coordinates   656913..657362 (-) Length   149 a.a.
NCBI ID   WP_024699421.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain FORC_023 isolate 378     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 651913..662362
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC23_RS03225 (FORC23_0543) coaE 652427..653041 (-) 615 WP_025523092.1 dephospho-CoA kinase -
  FORC23_RS03230 (FORC23_0544) pilD 653042..653911 (-) 870 WP_025523093.1 prepilin peptidase Machinery gene
  FORC23_RS03235 (FORC23_0545) pilC 653976..655199 (-) 1224 WP_025523095.1 type II secretion system F family protein Machinery gene
  FORC23_RS03240 (FORC23_0546) pilB 655224..656909 (-) 1686 WP_070225511.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FORC23_RS03245 (FORC23_0547) pilA 656913..657362 (-) 450 WP_024699421.1 pilin Machinery gene
  FORC23_RS03250 (FORC23_0548) nadC 657626..658513 (-) 888 WP_025523098.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FORC23_RS03255 (FORC23_0549) ampD 658606..659157 (+) 552 WP_005484832.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FORC23_RS03260 (FORC23_0550) pdhR 659563..660330 (+) 768 WP_005462576.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15609.83 Da        Isoelectric Point: 8.4834

>NTDB_id=158476 FORC23_RS03245 WP_024699421.1 656913..657362(-) (pilA) [Vibrio parahaemolyticus strain FORC_023 isolate 378]
MKHSKQKKQQGFTLIELMIVVAVIGVLAAIAIPQYQNYVAKSELGAGLATITSVRTNVEDYIVTNGSFPDGKTAGQLTTD
LGVIQPNNGAITFDDANSNILLTFAGSGNSPDVNNAKIALNRAAGGTWTCKATLDAKLLPKSCSKDANL

Nucleotide


Download         Length: 450 bp        

>NTDB_id=158476 FORC23_RS03245 WP_024699421.1 656913..657362(-) (pilA) [Vibrio parahaemolyticus strain FORC_023 isolate 378]
ATGAAACACAGTAAACAGAAAAAACAGCAAGGTTTTACGCTAATTGAATTGATGATTGTGGTGGCGGTAATTGGTGTTTT
GGCGGCGATTGCTATCCCTCAATACCAAAACTATGTGGCCAAGTCAGAGTTGGGGGCTGGACTAGCAACAATAACCTCAG
TTCGAACTAATGTTGAAGATTACATTGTTACAAATGGTAGTTTTCCTGATGGCAAAACTGCTGGCCAGCTAACAACAGAC
TTAGGAGTGATTCAGCCAAACAATGGTGCAATAACTTTTGATGACGCCAATAGCAACATCCTGTTAACTTTTGCTGGCTC
TGGAAATAGCCCTGACGTAAATAATGCAAAAATAGCACTAAACAGAGCGGCTGGTGGTACATGGACATGTAAAGCCACCC
TAGATGCTAAACTACTTCCAAAATCATGTTCTAAGGATGCTAATTTGTAG

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(40-143)

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

51.634

100

0.53
  pilA Vibrio cholerae strain A1552

51.634

100

0.53
  pilA Vibrio cholerae C6706

51.634

100

0.53
  pilA Pseudomonas aeruginosa PAK

44.966

100

0.45
  pilA Vibrio parahaemolyticus RIMD 2210633

47.794

91.275

0.436
  pilA/pilAI Pseudomonas stutzeri DSM 10701

42.361

96.644

0.409
  pilA Acinetobacter baumannii strain A118

39.583

96.644

0.383
  pilA Haemophilus influenzae Rd KW20

35.948

100

0.369
  pilA2 Legionella pneumophila str. Paris

39.855

92.617

0.369
  pilA Haemophilus influenzae 86-028NP

36.242

100

0.362
  pilA/pilA1 Eikenella corrodens VA1

33.333

100

0.362
  pilA2 Legionella pneumophila strain ERS1305867

39.13

92.617

0.362

Multiple sequence alignment