Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   FORC23_RS03235 Genome accession   NZ_CP012950
Coordinates   653976..655199 (-) Length   407 a.a.
NCBI ID   WP_025523095.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain FORC_023 isolate 378     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 648976..660199
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC23_RS03195 (FORC23_0537) rpsP 649082..649330 (+) 249 WP_005379962.1 30S ribosomal protein S16 -
  FORC23_RS03200 (FORC23_0538) rimM 649359..649907 (+) 549 WP_005462552.1 ribosome maturation factor RimM -
  FORC23_RS03205 (FORC23_0539) trmD 649935..650678 (+) 744 WP_005462562.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  FORC23_RS03210 (FORC23_0540) rplS 650720..651073 (+) 354 WP_005462554.1 50S ribosomal protein L19 -
  FORC23_RS03215 (FORC23_0541) yacG 651396..651590 (-) 195 WP_005462546.1 DNA gyrase inhibitor YacG -
  FORC23_RS03220 (FORC23_0542) zapD 651659..652399 (-) 741 WP_015297254.1 cell division protein ZapD -
  FORC23_RS03225 (FORC23_0543) coaE 652427..653041 (-) 615 WP_025523092.1 dephospho-CoA kinase -
  FORC23_RS03230 (FORC23_0544) pilD 653042..653911 (-) 870 WP_025523093.1 prepilin peptidase Machinery gene
  FORC23_RS03235 (FORC23_0545) pilC 653976..655199 (-) 1224 WP_025523095.1 type II secretion system F family protein Machinery gene
  FORC23_RS03240 (FORC23_0546) pilB 655224..656909 (-) 1686 WP_070225511.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FORC23_RS03245 (FORC23_0547) pilA 656913..657362 (-) 450 WP_024699421.1 pilin Machinery gene
  FORC23_RS03250 (FORC23_0548) nadC 657626..658513 (-) 888 WP_025523098.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FORC23_RS03255 (FORC23_0549) ampD 658606..659157 (+) 552 WP_005484832.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 407 a.a.        Molecular weight: 45237.43 Da        Isoelectric Point: 10.3996

>NTDB_id=158474 FORC23_RS03235 WP_025523095.1 653976..655199(-) (pilC) [Vibrio parahaemolyticus strain FORC_023 isolate 378]
MKTTTPQLKNFRWKGVNSSGKKTSGQTLAMSEIEVRERLDAQHIKIKKLKKSSISFLTKLSHRVKGKDITVFTRQISTML
VTGVPLVQALKLVSDNHKKAEMKSILMSVTRAVEAGTPMSKAMRTASNHFDPLYTDLIATGEQSGNLAEVFERLATYREK
NEQLRAKVIKALIYPAMVILVALGVSFIMLTKVIPEFEKMFVGFGAELPWFTRQVLDLSAWTQNWSPFIALGSISLFISA
RVLSMRSDSFRLMLNRSVLKFPVLGAVLSKAAIAKFSRTLATSFTAGIPILTSLKTTSKTSGNMHYQLAIEEVYRDTAAG
MPMYVAMRNCNVFPELVLQMVMIGEESGRLDDMLNKVATIYEFEVDNTVDNLSKILEPLIIVFLGIVVGGLVTAMYLPIF
NLMSVLG

Nucleotide


Download         Length: 1224 bp        

>NTDB_id=158474 FORC23_RS03235 WP_025523095.1 653976..655199(-) (pilC) [Vibrio parahaemolyticus strain FORC_023 isolate 378]
ATGAAAACTACGACACCACAATTAAAGAACTTCCGTTGGAAAGGCGTCAACAGCTCAGGGAAAAAGACCTCTGGTCAAAC
TCTGGCAATGAGCGAAATCGAAGTACGAGAGCGCTTAGATGCCCAGCACATCAAAATCAAAAAGCTAAAGAAAAGCAGCA
TCTCTTTTTTGACCAAACTTAGCCATCGCGTAAAAGGAAAAGACATCACGGTTTTCACTCGTCAAATCTCAACGATGCTA
GTAACCGGTGTGCCGCTAGTACAAGCATTGAAATTGGTCTCTGATAACCACAAAAAAGCGGAAATGAAATCCATTTTGAT
GAGTGTAACTCGGGCTGTTGAAGCGGGTACGCCGATGTCCAAAGCGATGCGTACCGCCAGCAACCATTTTGACCCGCTAT
ATACCGACCTGATTGCGACAGGAGAACAATCGGGCAACTTAGCCGAAGTGTTCGAACGCTTAGCTACCTACAGGGAAAAA
AATGAGCAGCTTAGAGCCAAAGTGATCAAGGCTCTAATATATCCAGCCATGGTTATTCTAGTTGCACTGGGAGTATCGTT
TATCATGCTAACCAAGGTCATTCCCGAGTTTGAAAAAATGTTTGTTGGCTTTGGCGCTGAATTGCCATGGTTTACAAGGC
AAGTCTTAGATCTTTCCGCTTGGACACAAAACTGGAGCCCATTTATCGCACTTGGCTCCATCAGTTTATTTATCTCCGCG
AGAGTACTCTCTATGCGTTCTGACTCTTTTCGCTTAATGCTCAACCGCTCTGTGCTTAAGTTTCCAGTCCTTGGGGCAGT
TTTATCGAAAGCCGCTATCGCAAAATTTAGTCGAACCCTCGCTACGAGCTTTACGGCGGGCATTCCGATTTTAACCAGTT
TAAAAACCACCTCGAAAACGTCCGGAAATATGCATTATCAATTGGCTATCGAAGAGGTTTATCGCGATACCGCAGCAGGA
ATGCCGATGTATGTTGCAATGCGCAATTGCAACGTGTTCCCTGAGTTGGTGCTGCAAATGGTTATGATCGGCGAAGAATC
CGGCCGACTTGATGACATGCTCAATAAAGTCGCCACAATTTACGAGTTTGAAGTGGACAACACCGTCGATAACCTCAGTA
AAATCTTAGAGCCATTGATTATCGTTTTTTTAGGTATCGTTGTCGGTGGCTTGGTCACAGCAATGTACTTGCCAATCTTT
AACTTAATGAGTGTATTGGGCTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-398)

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio campbellii strain DS40M4

86.241

100

0.862
  pilC Vibrio cholerae strain A1552

74.074

99.509

0.737
  pilC Acinetobacter baylyi ADP1

42.75

98.28

0.42
  pilC Acinetobacter baumannii D1279779

41.191

99.017

0.408
  pilC Legionella pneumophila strain ERS1305867

40.342

100

0.405
  pilG Neisseria gonorrhoeae MS11

40.494

99.509

0.403
  pilC Pseudomonas stutzeri DSM 10701

40.909

97.297

0.398
  pilG Neisseria meningitidis 44/76-A

39.901

99.754

0.398

Multiple sequence alignment