Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CDOMC_RS07440 Genome accession   NZ_CP012552
Coordinates   1402354..1403436 (-) Length   360 a.a.
NCBI ID   WP_172129030.1    Uniprot ID   A0A6G5R4U5
Organism   Campylobacter sp. RM16192     
Function   pseudopilus biogenesis (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1397354..1408436
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CDOMC_RS07415 (CDOMC_1458) - 1397914..1398456 (-) 543 WP_172129020.1 DUF1882 domain-containing protein -
  CDOMC_RS07420 (CDOMC_1459) - 1398456..1399700 (-) 1245 WP_172129022.1 serine hydroxymethyltransferase -
  CDOMC_RS07425 (CDOMC_1460) lysS 1399703..1401205 (-) 1503 WP_236861378.1 lysine--tRNA ligase -
  CDOMC_RS07430 (CDOMC_1461) - 1401226..1401681 (-) 456 WP_172129026.1 Fur family transcriptional regulator -
  CDOMC_RS07435 (CDOMC_1462) - 1401687..1402352 (-) 666 WP_172129028.1 CvpA family protein -
  CDOMC_RS07440 (CDOMC_1463) pilT 1402354..1403436 (-) 1083 WP_172129030.1 type IV pilus twitching motility protein PilT Machinery gene
  CDOMC_RS07445 (CDOMC_1464) gatC 1403442..1403729 (-) 288 WP_172129032.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC -
  CDOMC_RS07450 (CDOMC_1465) - 1403852..1404418 (+) 567 WP_172129034.1 hypothetical protein -
  CDOMC_RS07455 (CDOMC_1466) - 1404415..1405425 (+) 1011 WP_172129036.1 L-seryl-tRNA selenium transferase -
  CDOMC_RS07460 (CDOMC_1467) - 1405427..1405756 (+) 330 WP_172129038.1 hypothetical protein -
  CDOMC_RS07465 (CDOMC_1468) - 1405743..1406366 (+) 624 WP_172129040.1 type III pantothenate kinase -
  CDOMC_RS07470 (CDOMC_1469) hisG 1406363..1406977 (+) 615 WP_172129042.1 ATP phosphoribosyltransferase -
  CDOMC_RS07475 (CDOMC_1470) dapE 1406982..1408085 (-) 1104 WP_172129044.1 succinyl-diaminopimelate desuccinylase -

Sequence

Protein


Download         Length: 360 a.a.        Molecular weight: 39894.62 Da        Isoelectric Point: 6.5047

>NTDB_id=155135 CDOMC_RS07440 WP_172129030.1 1402354..1403436(-) (pilT) [Campylobacter sp. RM16192]
MQKYSDSNISLEALLRTVIHNKASDLHLVSRGEPQVRIDGALRPLELGVLNSNDIQDLCYALITDAQKSELEENRELDYA
IDLPNIGRFRANYYYTMNGELAAAFRIIPTEMPTLDGIGAPSIFKQIIKREKGMILVTGPTGSGKSTTLAAILNEINVTE
RKHILTIEDPVEFVHKNQKSLFSHRNIGTDTHSYTQALKYAMREDPDIILVGEIRDSETISTAITAAETGHLVFATLHTN
SAIQTINRIIDSFEGGEQLQVRNMLSVSLGAVISQSLIPKNGGGRLAVHEILINNPAVSNLIRENKIHQIYSQMQLNQQS
TGMMTQTQSLMKAIKNNLITKEMAMRYSTAPQELGNLLGI

Nucleotide


Download         Length: 1083 bp        

>NTDB_id=155135 CDOMC_RS07440 WP_172129030.1 1402354..1403436(-) (pilT) [Campylobacter sp. RM16192]
ATGCAAAAGTATAGTGATAGTAACATAAGCCTAGAAGCACTCCTAAGAACAGTGATTCACAATAAGGCAAGCGATCTTCA
CCTTGTGTCAAGAGGAGAGCCTCAAGTAAGAATAGATGGAGCTTTACGTCCGCTTGAGTTAGGCGTGCTTAATAGTAATG
ATATACAAGATTTGTGTTATGCCCTTATTACTGATGCTCAAAAAAGTGAACTTGAAGAGAATAGAGAGCTTGATTATGCT
ATAGATCTTCCTAATATAGGTCGTTTTAGGGCAAATTATTATTATACAATGAATGGTGAATTGGCTGCTGCCTTTCGTAT
AATACCAACAGAAATGCCAACTCTTGATGGCATTGGAGCTCCTAGTATTTTTAAACAAATTATAAAGCGTGAAAAAGGAA
TGATACTTGTTACTGGTCCTACAGGAAGCGGTAAATCAACAACTTTGGCAGCTATATTAAATGAAATAAATGTAACCGAA
AGAAAACATATCCTTACTATTGAAGATCCTGTTGAGTTCGTTCATAAAAATCAAAAATCATTATTTTCTCATAGAAATAT
AGGTACAGATACACATTCTTATACACAAGCTCTTAAATATGCCATGCGTGAAGATCCAGATATTATACTGGTTGGTGAGA
TAAGGGATAGCGAAACTATATCTACTGCTATTACAGCAGCAGAAACAGGACACCTTGTGTTTGCAACACTTCACACAAAC
TCTGCTATTCAGACTATTAACCGTATTATAGATAGTTTTGAGGGTGGTGAACAGCTTCAAGTTAGAAATATGCTTTCTGT
TTCATTAGGTGCAGTGATTTCACAAAGTTTGATACCAAAAAATGGTGGAGGTCGTTTGGCTGTTCATGAAATTTTAATCA
ACAATCCAGCTGTTTCAAATTTAATTAGAGAAAATAAAATTCACCAAATTTACTCTCAAATGCAATTAAATCAGCAATCT
ACAGGTATGATGACACAGACTCAATCTTTAATGAAAGCTATTAAGAACAATCTTATCACAAAAGAGATGGCTATGAGATA
TTCAACTGCTCCACAAGAACTTGGAAATTTACTAGGGATATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(16-251)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6G5R4U5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria gonorrhoeae MS11

52.584

91.389

0.481
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

53.067

90.556

0.481
  pilT Vibrio cholerae strain A1552

53.067

90.556

0.481
  pilT Neisseria meningitidis 8013

52.28

91.389

0.478
  pilT Acinetobacter nosocomialis M2

49.56

94.722

0.469
  pilT Acinetobacter baumannii D1279779

49.56

94.722

0.469
  pilT Acinetobacter baumannii strain A118

49.56

94.722

0.469
  pilT Legionella pneumophila strain Lp02

50.453

91.944

0.464
  pilT Legionella pneumophila strain ERS1305867

50.453

91.944

0.464
  pilT Acinetobacter baylyi ADP1

48.387

94.722

0.458
  pilT Pseudomonas aeruginosa PAK

48.485

91.667

0.444
  pilT Pseudomonas stutzeri DSM 10701

48.182

91.667

0.442
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.898

97.778

0.419
  pilU Vibrio cholerae strain A1552

37.356

96.667

0.361

Multiple sequence alignment