Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AA650_RS16895 Genome accession   NZ_CP011456
Coordinates   3880428..3881528 (+) Length   366 a.a.
NCBI ID   WP_053539894.1    Uniprot ID   -
Organism   Anabaena sp. WA102 isolate     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3875428..3886528
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AA650_RS16875 (AA650_16955) - 3875870..3876241 (-) 372 WP_053539892.1 hypothetical protein -
  AA650_RS16880 (AA650_16960) - 3876367..3876882 (-) 516 WP_234413214.1 IS607 family transposase -
  AA650_RS16885 (AA650_16965) grpE 3877032..3877706 (-) 675 WP_039202898.1 nucleotide exchange factor GrpE -
  AA650_RS16890 (AA650_16970) pilB/pilB1 3878363..3880363 (+) 2001 WP_053539893.1 GspE/PulE family protein Machinery gene
  AA650_RS16895 (AA650_16975) pilT 3880428..3881528 (+) 1101 WP_053539894.1 type IV pilus twitching motility protein PilT Machinery gene
  AA650_RS16900 (AA650_16980) - 3881646..3882869 (+) 1224 WP_039203041.1 type II secretion system F family protein -
  AA650_RS16905 (AA650_16985) - 3882939..3884465 (+) 1527 WP_053539895.1 thymidylate synthase -
  AA650_RS16910 (AA650_16990) - 3884485..3884763 (+) 279 WP_234413215.1 DUF4351 domain-containing protein -
  AA650_RS16915 (AA650_16995) - 3884773..3885612 (+) 840 WP_053539897.1 DUF2887 domain-containing protein -

Sequence

Protein


Download         Length: 366 a.a.        Molecular weight: 40177.43 Da        Isoelectric Point: 7.1091

>NTDB_id=146142 AA650_RS16895 WP_053539894.1 3880428..3881528(+) (pilT) [Anabaena sp. WA102 isolate]
MEMMIEDLMEQLINMGGSDLHLSAGLPPYFRISGHLTPIGEHTLTADECQRLIFSMLNNTQRKTLEQTWELDCSYGVKGL
ARFRVNVYKERGAFAACLRALSSKIPNFEKLGLPDIVREMTEKPRGLILVTGPTGSGKTTTLAAMIDLINRTRAEHILTV
EDPVEFVYEPIKSLVHQRQLGEDTKSFANALKAALREDPDIILVGEMRDLETIALAISAAETGHLVFGTLHTSSAAQTVD
RIIDVFPSERQTQVRVQLSNSLVAVFSQTLVPKKNPKANEYGRVMAQEIMVITPAISNLIREGKTAQIYSAIQTGGKLGM
QTLEKVLADLYKAGTISFEAAISKTSKPDEVQRLIGATAPAGAKLR

Nucleotide


Download         Length: 1101 bp        

>NTDB_id=146142 AA650_RS16895 WP_053539894.1 3880428..3881528(+) (pilT) [Anabaena sp. WA102 isolate]
ATGGAAATGATGATTGAAGACTTGATGGAGCAACTAATTAATATGGGTGGCTCAGACTTACACCTATCTGCTGGTTTGCC
TCCCTATTTTCGGATTAGTGGACATCTAACACCTATTGGTGAACATACCTTAACTGCTGATGAGTGCCAAAGGTTAATTT
TTAGTATGCTCAATAACACTCAGCGCAAAACCTTAGAGCAAACCTGGGAATTAGATTGTTCTTATGGTGTAAAAGGATTA
GCTCGGTTTCGGGTGAATGTCTACAAAGAGCGGGGTGCTTTTGCTGCTTGTTTACGAGCATTAAGTTCTAAAATTCCTAA
CTTTGAAAAATTAGGTTTGCCCGATATAGTTCGGGAAATGACGGAAAAACCTAGAGGATTAATTCTCGTTACAGGTCCAA
CTGGTTCTGGTAAAACTACTACCTTAGCGGCAATGATTGACCTAATTAATCGGACTAGGGCAGAACACATTTTAACGGTT
GAAGATCCTGTAGAATTTGTTTATGAACCTATCAAAAGTCTAGTCCACCAACGCCAATTAGGGGAAGATACGAAGAGTTT
TGCTAATGCTTTGAAAGCAGCTTTACGGGAAGATCCAGATATTATTCTCGTAGGAGAAATGCGGGATTTAGAAACAATTG
CTTTGGCAATTTCCGCCGCAGAAACAGGTCACTTGGTATTTGGTACATTACATACAAGCTCTGCTGCACAAACCGTTGAC
CGGATTATTGACGTTTTCCCCTCAGAAAGACAAACTCAGGTGCGGGTGCAGTTATCTAACTCCTTAGTTGCAGTTTTTAG
TCAAACTTTAGTACCTAAGAAAAATCCCAAAGCTAATGAATATGGTCGAGTTATGGCTCAAGAAATCATGGTGATTACTC
CCGCTATTTCTAACTTGATTCGGGAAGGAAAAACAGCACAGATTTACTCAGCTATTCAGACTGGTGGTAAATTGGGAATG
CAAACTCTAGAAAAGGTTTTGGCTGATTTATACAAAGCTGGAACTATTTCTTTTGAAGCGGCAATCTCTAAAACCTCTAA
ACCAGATGAAGTCCAGCGTCTAATTGGTGCGACAGCCCCAGCAGGAGCTAAATTGCGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(44-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

54.467

94.809

0.516
  pilT Pseudomonas stutzeri DSM 10701

54.179

94.809

0.514
  pilT Legionella pneumophila strain ERS1305867

53.89

94.809

0.511
  pilT Acinetobacter baylyi ADP1

53.89

94.809

0.511
  pilT Legionella pneumophila strain Lp02

53.89

94.809

0.511
  pilT Acinetobacter nosocomialis M2

53.026

94.809

0.503
  pilT Acinetobacter baumannii D1279779

53.026

94.809

0.503
  pilT Acinetobacter baumannii strain A118

53.026

94.809

0.503
  pilT Neisseria gonorrhoeae MS11

51.622

92.623

0.478
  pilT Neisseria meningitidis 8013

51.327

92.623

0.475
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.292

93.443

0.47
  pilT Vibrio cholerae strain A1552

50.292

93.443

0.47
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.857

95.628

0.467
  pilU Acinetobacter baylyi ADP1

37.432

100

0.374

Multiple sequence alignment