Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   AA650_RS16890 Genome accession   NZ_CP011456
Coordinates   3878363..3880363 (+) Length   666 a.a.
NCBI ID   WP_053539893.1    Uniprot ID   -
Organism   Anabaena sp. WA102 isolate     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3873363..3885363
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AA650_RS16870 (AA650_16950) dnaK 3873725..3875683 (-) 1959 WP_053539891.1 molecular chaperone DnaK -
  AA650_RS16875 (AA650_16955) - 3875870..3876241 (-) 372 WP_053539892.1 hypothetical protein -
  AA650_RS16880 (AA650_16960) - 3876367..3876882 (-) 516 WP_234413214.1 IS607 family transposase -
  AA650_RS16885 (AA650_16965) grpE 3877032..3877706 (-) 675 WP_039202898.1 nucleotide exchange factor GrpE -
  AA650_RS16890 (AA650_16970) pilB/pilB1 3878363..3880363 (+) 2001 WP_053539893.1 GspE/PulE family protein Machinery gene
  AA650_RS16895 (AA650_16975) pilT 3880428..3881528 (+) 1101 WP_053539894.1 type IV pilus twitching motility protein PilT Machinery gene
  AA650_RS16900 (AA650_16980) - 3881646..3882869 (+) 1224 WP_039203041.1 type II secretion system F family protein -
  AA650_RS16905 (AA650_16985) - 3882939..3884465 (+) 1527 WP_053539895.1 thymidylate synthase -
  AA650_RS16910 (AA650_16990) - 3884485..3884763 (+) 279 WP_234413215.1 DUF4351 domain-containing protein -

Sequence

Protein


Download         Length: 666 a.a.        Molecular weight: 74774.82 Da        Isoelectric Point: 5.3492

>NTDB_id=146141 AA650_RS16890 WP_053539893.1 3878363..3880363(+) (pilB/pilB1) [Anabaena sp. WA102 isolate]
MTYSSPQGRSTAITIRTEFSPFANKLVASGYINNEQMRQALAMSRKTGKLLTEVLESITDRKLAPEQVTAYKKQHLFELK
ILYGIESLDTEINEIDTTNVGMLIQELIPVELCRRHYLVPLSRHDDQPSPYILMAMVDPDNLEASDDLNRILRPKGLSFQ
RRVITQKDYLGIINQYLNEQAARQKTLDQQKNYDVKGDLDNLENLEIEESSDDDVDLDKAMKGADDAPIIKLVNRILLKA
LSEGVSDIHIEPQEEYLRIRFRKDGVLREAFENMPKKIIPAVTARFKIISNLDIAERRLPQDGRIRRIFAGRKVDFRVNT
LPSRYGEKVVLRILDNSSTQLGLDKLITDPETLKIVQDMVSKPFGLILVTGPTGSGKTTSLYSALSEKNDPGINISTVED
PIEYSLPGITQVQVIREKGLDFSTALRAFLRQDPDVLLVGETRDKETAKTAIEAALTGHLVLTTLHTNDAPGAIARLGEM
GIEPFMVSSSLIGVLAQRLVRRVCSECRIAYSPTIEELARYGLSASQEAQVTFYKANIIPPEEIVSRKNLCPKCNGIGYK
GRCGVYEVMRITEELQTLINKEAPTERIKEVAVEEGMKTLLAYSLDLVRQGATTLEEVERVTFTDTGLEAELKAKRKSGL
TCRTCTGELQPEWLDCPYCMTPRFTD

Nucleotide


Download         Length: 2001 bp        

>NTDB_id=146141 AA650_RS16890 WP_053539893.1 3878363..3880363(+) (pilB/pilB1) [Anabaena sp. WA102 isolate]
ATGACTTACTCATCACCACAAGGGCGCAGTACGGCGATAACTATCAGAACTGAGTTTTCGCCCTTTGCTAATAAACTAGT
AGCATCTGGCTATATCAATAATGAACAAATGAGACAGGCTTTAGCTATGAGCCGCAAGACTGGCAAGCTACTGACAGAAG
TATTAGAGTCAATTACTGACCGAAAATTAGCTCCTGAACAGGTAACAGCATACAAAAAACAACACTTATTTGAACTTAAA
ATTTTATACGGTATTGAGTCGCTTGATACTGAAATCAACGAAATTGATACAACAAATGTGGGGATGCTGATTCAAGAACT
CATTCCTGTGGAGCTATGTCGTCGCCATTATCTAGTGCCATTGTCAAGACATGATGACCAACCGTCACCTTATATTTTAA
TGGCGATGGTTGACCCAGATAATTTAGAGGCTTCTGATGATCTTAACCGAATTTTGCGTCCTAAAGGTTTGTCTTTTCAG
CGGCGGGTAATTACGCAGAAAGATTACTTGGGCATTATTAATCAATACTTAAATGAGCAAGCTGCCCGCCAAAAGACCTT
AGATCAACAAAAAAATTATGATGTTAAGGGGGATTTAGACAATTTAGAAAATCTGGAAATAGAGGAAAGTAGTGATGATG
ATGTTGATTTAGATAAGGCGATGAAGGGTGCTGATGATGCACCTATTATTAAACTTGTGAATCGGATTTTGTTGAAGGCT
TTAAGTGAGGGTGTTTCGGATATTCACATTGAACCCCAGGAAGAATATTTACGGATTCGTTTCCGTAAGGATGGGGTATT
ACGTGAGGCATTTGAAAATATGCCGAAAAAAATTATCCCGGCAGTAACAGCCAGATTTAAAATTATTTCTAATTTGGACA
TTGCGGAGAGACGTTTACCTCAAGATGGACGTATCCGCCGGATATTTGCGGGGAGAAAGGTGGATTTTCGGGTAAATACT
CTACCTAGTCGTTATGGAGAAAAGGTTGTTCTCCGGATTTTAGATAACTCCTCTACTCAATTGGGTTTAGATAAGTTAAT
TACTGACCCAGAAACCTTAAAAATTGTTCAGGATATGGTGAGTAAGCCCTTTGGGTTGATTTTGGTGACAGGACCAACTG
GTTCGGGTAAAACGACTTCGTTGTATTCGGCACTGTCAGAAAAGAATGATCCGGGGATTAATATTAGTACGGTAGAAGAT
CCGATTGAGTACAGTTTACCGGGGATTACTCAAGTACAGGTAATTCGGGAAAAGGGGTTGGATTTCTCGACGGCTTTGCG
GGCTTTTTTACGTCAAGATCCAGATGTGTTACTGGTGGGGGAAACACGAGATAAGGAAACTGCAAAAACGGCGATTGAGG
CGGCATTAACCGGTCACTTGGTATTAACTACTTTACACACTAATGATGCGCCGGGAGCGATCGCTCGCTTAGGAGAAATG
GGTATTGAACCGTTCATGGTTTCCAGTTCTCTCATTGGTGTTTTAGCACAACGTCTAGTTCGTCGTGTCTGTTCTGAATG
TCGGATTGCCTATAGTCCCACCATTGAAGAACTCGCTCGCTATGGTTTATCAGCTTCCCAAGAGGCTCAGGTGACTTTCT
ATAAAGCCAATATTATCCCACCAGAAGAAATAGTCAGCCGTAAAAATCTTTGTCCAAAATGTAATGGCATTGGCTACAAG
GGGCGTTGTGGTGTTTATGAAGTGATGCGGATTACTGAAGAATTACAAACCCTTATTAACAAGGAAGCACCTACAGAAAG
GATCAAAGAAGTGGCTGTTGAAGAGGGTATGAAAACCTTACTGGCATACAGTTTAGACCTAGTTCGCCAAGGTGCAACTA
CCCTAGAAGAGGTAGAACGGGTAACATTTACAGACACAGGTTTAGAAGCTGAATTAAAGGCTAAACGCAAAAGTGGTCTG
ACTTGTCGGACTTGTACAGGGGAATTACAACCAGAATGGTTAGATTGTCCTTACTGTATGACACCTCGATTTACAGATTA
A

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(26-181)

Type II/IV secretion system protein (T2SSE)

(235-502)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

63.893

100

0.646

Multiple sequence alignment