Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AAW31_RS08130 Genome accession   NZ_CP011451
Coordinates   1785516..1786652 (+) Length   378 a.a.
NCBI ID   WP_046849852.1    Uniprot ID   A0A0F7KBG4
Organism   Nitrosomonas communis strain Nm2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1780516..1791652
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAW31_RS08100 (AAW31_08100) - 1780697..1781944 (-) 1248 WP_046849847.1 lipoprotein-releasing ABC transporter permease subunit -
  AAW31_RS08105 (AAW31_08105) rsmD 1782051..1782671 (+) 621 WP_309567611.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  AAW31_RS08110 (AAW31_08110) coaD 1782664..1783143 (+) 480 WP_046849848.1 pantetheine-phosphate adenylyltransferase -
  AAW31_RS08115 (AAW31_08115) - 1783162..1783431 (+) 270 WP_046849849.1 YfhL family 4Fe-4S dicluster ferredoxin -
  AAW31_RS08120 (AAW31_08120) - 1783400..1784116 (-) 717 WP_046849850.1 YggS family pyridoxal phosphate-dependent enzyme -
  AAW31_RS08125 (AAW31_08125) pilT 1784337..1785380 (+) 1044 WP_046849851.1 type IV pilus twitching motility protein PilT Machinery gene
  AAW31_RS08130 (AAW31_08130) pilU 1785516..1786652 (+) 1137 WP_046849852.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AAW31_RS08135 (AAW31_08135) - 1786794..1787585 (-) 792 WP_046849853.1 carbonic anhydrase -
  AAW31_RS08140 (AAW31_08140) - 1787631..1787930 (+) 300 WP_158441449.1 hypothetical protein -
  AAW31_RS08145 (AAW31_08145) - 1788151..1788645 (-) 495 WP_046849855.1 hypothetical protein -
  AAW31_RS23565 (AAW31_08150) - 1789082..1790224 (-) 1143 Protein_1717 aldehyde dehydrogenase family protein -
  AAW31_RS08155 (AAW31_08155) - 1790272..1791381 (-) 1110 WP_046849856.1 NAD(P)-dependent alcohol dehydrogenase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42224.65 Da        Isoelectric Point: 6.5045

>NTDB_id=146111 AAW31_RS08130 WP_046849852.1 1785516..1786652(+) (pilU) [Nitrosomonas communis strain Nm2]
MEQEQATKFMHDLLHLMLSKKASDLFITAGYPPAFKIDGNMTPVSQQPLSPQQTAELARAIMNDKQAAEFAATKECNFAI
HPAGIGRFRVNAFIQQQRVGLVIRTITTQIPKFDDLGLPLVLKDVVMSKRGLVIFAGGTGSGKSTSLAALIGHRNQNSYG
HIITIEDPVEYVHEHINCVITQREVGVDTDSWEAALKNTLRQAPDLILIGEIRDRETMEHAIAFAETGHLCMGTLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRALIAQRLIPRKEGKGRVAAIEVMLNSPLISDLIFKGEVHEIKEIMKKSRELG
MQTFDMALFELYEAGLISYEEALRNADSMNELRLQIKLHSSEAQHKNINEGIEHLTIT

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=146111 AAW31_RS08130 WP_046849852.1 1785516..1786652(+) (pilU) [Nitrosomonas communis strain Nm2]
ATGGAACAAGAGCAGGCAACAAAATTTATGCACGATTTGCTGCATTTAATGCTCAGCAAAAAGGCTTCCGATTTGTTTAT
CACAGCTGGCTATCCGCCTGCGTTTAAAATTGACGGAAATATGACCCCCGTATCCCAGCAGCCGCTTTCCCCCCAGCAAA
CGGCAGAACTGGCGCGCGCTATCATGAATGACAAACAGGCGGCAGAGTTTGCAGCGACCAAGGAGTGCAATTTTGCCATT
CATCCAGCGGGCATCGGACGATTTCGTGTGAATGCATTCATTCAACAACAGCGAGTAGGTCTTGTGATTCGCACGATCAC
TACACAAATTCCAAAATTTGATGATTTGGGTCTGCCGCTTGTGCTAAAAGACGTGGTGATGAGCAAGCGTGGTTTAGTTA
TTTTTGCAGGCGGTACCGGCTCAGGTAAATCGACCTCACTTGCCGCTCTGATCGGACACCGTAATCAGAACAGTTATGGC
CATATCATCACTATTGAAGACCCAGTAGAATATGTGCATGAGCATATTAACTGCGTTATCACACAACGAGAAGTGGGCGT
GGATACGGATTCCTGGGAAGCAGCACTCAAGAATACCTTGCGCCAGGCACCTGATCTTATTTTGATTGGTGAAATTCGTG
ATCGTGAAACCATGGAGCATGCCATTGCTTTTGCCGAGACGGGCCACTTATGCATGGGGACGCTGCACGCCAACAGTGCC
AACCAGGCGCTTGACCGCATTATTAATTTTTTTCCGGAAGAACGCCGAGCACAGCTGTTGATGGATTTATCATTAAATTT
GAGAGCCTTGATTGCGCAACGGCTGATTCCCAGGAAAGAGGGGAAAGGGCGGGTGGCAGCAATTGAGGTGATGCTGAATT
CTCCATTGATCTCAGATCTGATTTTTAAAGGTGAAGTTCATGAAATCAAAGAGATCATGAAAAAATCGCGCGAGCTCGGT
ATGCAAACTTTTGATATGGCGTTATTTGAGCTTTATGAAGCTGGCCTGATCAGTTATGAGGAAGCGCTGAGAAATGCAGA
TTCCATGAATGAGCTACGCTTACAAATCAAACTGCACAGCTCAGAAGCGCAACACAAAAACATCAATGAAGGCATTGAGC
ATTTGACGATTACCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(122-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0F7KBG4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.878

95.767

0.593
  pilU Acinetobacter baylyi ADP1

61.69

93.915

0.579
  pilU Vibrio cholerae strain A1552

56.891

90.212

0.513
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.478

88.624

0.394
  pilT Pseudomonas aeruginosa PAK

44.478

88.624

0.394
  pilT Pseudomonas stutzeri DSM 10701

42.388

88.624

0.376
  pilT Legionella pneumophila strain ERS1305867

42.9

87.566

0.376
  pilT Legionella pneumophila strain Lp02

42.9

87.566

0.376
  pilT Acinetobacter nosocomialis M2

41.994

87.566

0.368
  pilT Acinetobacter baumannii D1279779

41.994

87.566

0.368
  pilT Acinetobacter baumannii strain A118

41.994

87.566

0.368
  pilT Acinetobacter baylyi ADP1

41.994

87.566

0.368
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.818

87.302

0.365
  pilT Vibrio cholerae strain A1552

41.818

87.302

0.365

Multiple sequence alignment