Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AAW51_RS26935 Genome accession   NZ_CP011371
Coordinates   6315903..6317039 (-) Length   378 a.a.
NCBI ID   WP_047197083.1    Uniprot ID   A0A0G3BRP4
Organism   Caldimonas brevitalea strain DSM 7029     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6310903..6322039
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAW51_RS26915 (AAW51_5404) - 6311577..6312563 (+) 987 WP_047197081.1 HlyD family secretion protein -
  AAW51_RS26920 (AAW51_5405) - 6312577..6313767 (+) 1191 WP_238947706.1 ABC transporter permease -
  AAW51_RS26925 (AAW51_5406) - 6313764..6314930 (+) 1167 WP_053014065.1 ABC transporter permease -
  AAW51_RS26930 (AAW51_5407) - 6314908..6315807 (-) 900 WP_047197082.1 NAD(P)-dependent oxidoreductase -
  AAW51_RS26935 (AAW51_5408) pilU 6315903..6317039 (-) 1137 WP_047197083.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AAW51_RS26940 (AAW51_5409) - 6317125..6317778 (-) 654 WP_047197084.1 cyclic nucleotide-binding domain-containing protein -
  AAW51_RS26945 (AAW51_5410) pilT 6317834..6318877 (-) 1044 WP_047197085.1 type IV pilus twitching motility protein PilT Machinery gene
  AAW51_RS26950 (AAW51_5411) - 6318930..6319634 (+) 705 WP_047197086.1 YggS family pyridoxal phosphate-dependent enzyme -
  AAW51_RS26955 (AAW51_5412) - 6319676..6320158 (-) 483 WP_047197087.1 Lrp/AsnC ligand binding domain-containing protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42047.32 Da        Isoelectric Point: 6.7013

>NTDB_id=145192 AAW51_RS26935 WP_047197083.1 6315903..6317039(-) (pilU) [Caldimonas brevitalea strain DSM 7029]
MERDQASKFINDLLRLMISRNGSDLFLTAEFPPAIKVDGRVTKVSPQPLTGQHTLALARSIMNDKQAAEFERTKECNFAV
APQGVGRFRVNAFVQQGQVGLVMRVIPQTLPNIDAMGLPQVLKEVTMTKRGLVILVGATGSGKSTTLAAMVDYRNENSYG
HIITIEDPVEFVHPHKNCIVTQREVGLDTEGWGQALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCMATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLKSMVSQRLLPRQEGKGRVAAVEILLNTPLISELIFKGEVAEIKEIMKKSRELG
MQTFDQSLFDLYEGNQVTYEDALRNADSVNDLRLQIKLNSNRARSSDLAAGTEHLTIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=145192 AAW51_RS26935 WP_047197083.1 6315903..6317039(-) (pilU) [Caldimonas brevitalea strain DSM 7029]
ATGGAAAGAGACCAGGCTTCAAAATTCATCAACGACTTGTTGCGCCTGATGATCAGCCGCAACGGGTCGGACTTGTTCCT
GACCGCTGAATTTCCGCCGGCGATCAAGGTCGACGGCCGCGTCACCAAGGTGTCGCCGCAGCCCCTGACCGGCCAGCACA
CGCTGGCCCTGGCACGTTCGATCATGAACGACAAGCAGGCGGCCGAATTCGAGCGCACCAAGGAATGCAATTTCGCAGTC
GCGCCCCAAGGTGTCGGCCGCTTCCGCGTCAACGCCTTCGTGCAGCAGGGGCAGGTCGGGCTGGTGATGCGGGTGATCCC
GCAGACGCTGCCCAACATCGATGCGATGGGCCTGCCGCAGGTGCTCAAGGAAGTCACGATGACCAAGCGTGGCCTGGTCA
TCCTGGTCGGGGCGACCGGCTCCGGCAAGTCGACCACGCTGGCGGCGATGGTCGACTACCGCAACGAAAACTCGTACGGC
CACATCATCACCATCGAAGATCCGGTCGAGTTCGTGCATCCGCACAAGAACTGCATCGTGACGCAGCGTGAAGTCGGGCT
CGACACCGAAGGCTGGGGCCAGGCGCTCAAGAACACGCTGCGGCAGGCGCCCGACGTGATCCTGATGGGCGAGATCCGCG
ACCGCGAGACGATGGAACATGCGGTGGCCTTCGCCGAGACCGGTCACCTGTGCATGGCCACGCTGCATGCCAACAGCGCC
AACCAGGCGCTCGACCGCATCATCAACTTCTTCCCCGAAGAGCGCCGGGCGCAGCTGTTGATGGACTTGTCGCTCAACCT
CAAGTCCATGGTGTCGCAGCGTCTGCTGCCGCGCCAGGAGGGCAAGGGCCGGGTCGCTGCGGTCGAGATCCTGCTCAACA
CGCCGCTGATCTCCGAGCTGATCTTCAAGGGCGAAGTCGCCGAGATCAAGGAGATCATGAAGAAGAGCCGCGAGCTGGGC
ATGCAGACCTTCGACCAGTCGCTGTTCGACCTCTACGAAGGCAACCAGGTCACCTACGAAGACGCGTTGCGCAATGCCGA
CTCGGTCAACGACCTGCGGCTGCAGATCAAGCTCAACAGCAACCGCGCCCGCAGCTCCGACCTGGCCGCCGGCACCGAGC
ACCTGACCATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(87-256)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0G3BRP4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.109

96.825

0.582
  pilU Acinetobacter baylyi ADP1

55.858

97.09

0.542
  pilU Vibrio cholerae strain A1552

52.893

96.032

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.478

89.153

0.423
  pilT Pseudomonas aeruginosa PAK

45.994

89.153

0.41
  pilT Pseudomonas stutzeri DSM 10701

44.214

89.153

0.394
  pilT Acinetobacter nosocomialis M2

45.455

84.392

0.384
  pilT Acinetobacter baumannii D1279779

45.455

84.392

0.384
  pilT Acinetobacter baumannii strain A118

45.455

84.392

0.384
  pilT Legionella pneumophila strain Lp02

43.243

88.095

0.381
  pilT Legionella pneumophila strain ERS1305867

43.243

88.095

0.381
  pilT Acinetobacter baylyi ADP1

44.514

84.392

0.376
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

44.025

84.127

0.37
  pilT Vibrio cholerae strain A1552

44.025

84.127

0.37

Multiple sequence alignment