Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AAW51_RS26945 Genome accession   NZ_CP011371
Coordinates   6317834..6318877 (-) Length   347 a.a.
NCBI ID   WP_047197085.1    Uniprot ID   A0A0G3BXK3
Organism   Caldimonas brevitalea strain DSM 7029     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6312834..6323877
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAW51_RS26925 (AAW51_5406) - 6313764..6314930 (+) 1167 WP_053014065.1 ABC transporter permease -
  AAW51_RS26930 (AAW51_5407) - 6314908..6315807 (-) 900 WP_047197082.1 NAD(P)-dependent oxidoreductase -
  AAW51_RS26935 (AAW51_5408) pilU 6315903..6317039 (-) 1137 WP_047197083.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AAW51_RS26940 (AAW51_5409) - 6317125..6317778 (-) 654 WP_047197084.1 cyclic nucleotide-binding domain-containing protein -
  AAW51_RS26945 (AAW51_5410) pilT 6317834..6318877 (-) 1044 WP_047197085.1 type IV pilus twitching motility protein PilT Machinery gene
  AAW51_RS26950 (AAW51_5411) - 6318930..6319634 (+) 705 WP_047197086.1 YggS family pyridoxal phosphate-dependent enzyme -
  AAW51_RS26955 (AAW51_5412) - 6319676..6320158 (-) 483 WP_047197087.1 Lrp/AsnC ligand binding domain-containing protein -
  AAW51_RS26960 (AAW51_5413) - 6320256..6323267 (+) 3012 WP_238947707.1 L-glutamate gamma-semialdehyde dehydrogenase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38196.85 Da        Isoelectric Point: 7.0670

>NTDB_id=145193 AAW51_RS26945 WP_047197085.1 6317834..6318877(-) (pilT) [Caldimonas brevitalea strain DSM 7029]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVDPLEHKQVHDMVYDIMNDSQRKAYEETLECDFSFEIQGLAR
FRVNAFNQNRGAGAVFRTIPSKILTLEQLNTPKIFAELALKPRGLVLVTGPTGSGKSTTLAAMVNHLNESEYGHVLTVED
PIEFVHESKKCLINQREVGPHTLSFSNALRSALREDPDAILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
VDVFPAAEKEMVRAMLSESVVAIISQTLCKLKDGSGRVAAHEVMLGTAAVRNLIRENKIAQMYSAIQTGNSMGMQTLDQN
LADLVRRNVISPSEARTKAKFPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=145193 AAW51_RS26945 WP_047197085.1 6317834..6318877(-) (pilT) [Caldimonas brevitalea strain DSM 7029]
ATGGACATCACCCAGCTGTTGGCGTTCTCCGTCAAAAACAAAGCCTCCGACTTGCACCTGTCGGCCGGGCTGCCGCCGAT
GATCCGCGTCCATGGCGACGTGCGCCGCATCAATGTCGACCCGCTCGAGCACAAGCAGGTGCACGACATGGTGTACGACA
TCATGAACGACTCGCAGCGCAAGGCCTACGAAGAGACGCTCGAGTGCGATTTCTCGTTCGAGATCCAGGGCCTGGCCCGT
TTCCGCGTCAACGCGTTCAACCAGAACCGCGGCGCGGGGGCGGTGTTCCGCACCATCCCGTCGAAGATCCTGACGCTCGA
GCAGCTCAACACGCCGAAGATCTTCGCCGAGCTGGCACTCAAGCCGCGCGGCCTGGTGCTGGTCACCGGGCCCACCGGCT
CGGGCAAGTCGACCACGCTGGCCGCGATGGTCAACCACTTGAACGAGAGCGAGTACGGCCACGTGCTGACGGTCGAAGAC
CCGATCGAGTTCGTGCACGAGTCGAAGAAGTGCTTGATCAACCAGCGTGAAGTCGGCCCGCACACGCTCAGCTTCAGCAA
TGCCTTGCGCTCCGCACTGCGCGAGGACCCGGACGCGATCCTGGTCGGCGAGATGCGCGACCTCGAAACCATCCGTTTGG
CCCTGACCGCCGCCGAAACCGGCCACCTGGTGTTCGGCACGTTGCACACCTCGAGTGCCGCCAAGACCATCGACCGGGTG
GTCGACGTGTTCCCGGCGGCCGAGAAAGAGATGGTGCGCGCAATGCTGTCCGAATCGGTCGTCGCGATCATCTCGCAGAC
GCTGTGCAAGCTGAAGGACGGCTCGGGCCGTGTCGCTGCACACGAGGTCATGCTCGGCACGGCCGCGGTGCGCAACCTGA
TCCGCGAGAACAAGATCGCCCAGATGTACTCGGCCATCCAGACCGGCAACAGCATGGGCATGCAGACGCTCGACCAGAAC
CTGGCCGACCTGGTGCGACGTAACGTCATCTCGCCCTCCGAGGCGCGTACCAAGGCCAAGTTCCCCGAGAACTTCCCGGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0G3BXK3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

76.522

99.424

0.761
  pilT Acinetobacter nosocomialis M2

76.232

99.424

0.758
  pilT Acinetobacter baumannii D1279779

76.232

99.424

0.758
  pilT Acinetobacter baumannii strain A118

76.232

99.424

0.758
  pilT Pseudomonas stutzeri DSM 10701

74.783

99.424

0.744
  pilT Acinetobacter baylyi ADP1

72.464

99.424

0.72
  pilT Legionella pneumophila strain Lp02

71.386

97.695

0.697
  pilT Legionella pneumophila strain ERS1305867

71.386

97.695

0.697
  pilT Neisseria meningitidis 8013

67.536

99.424

0.671
  pilT Neisseria gonorrhoeae MS11

67.246

99.424

0.669
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

65.976

97.406

0.643
  pilT Vibrio cholerae strain A1552

65.976

97.406

0.643
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

99.424

0.504
  pilU Acinetobacter baylyi ADP1

40.462

99.712

0.403
  pilU Pseudomonas stutzeri DSM 10701

40.896

96.542

0.395
  pilU Vibrio cholerae strain A1552

40.896

96.542

0.395

Multiple sequence alignment