Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CFTSP3_RS05895 Genome accession   NZ_CP010953
Coordinates   1189917..1190978 (-) Length   353 a.a.
NCBI ID   WP_023385428.1    Uniprot ID   A0AAX0HDF4
Organism   Campylobacter fetus subsp. testudinum Sp3     
Function   pseudopilus biogenesis (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1184917..1195978
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CFTSP3_RS05870 (CFTSP3_1180) - 1185570..1186112 (-) 543 WP_058909389.1 DUF1882 domain-containing protein -
  CFTSP3_RS05875 (CFTSP3_1181) - 1186112..1187356 (-) 1245 WP_039362597.1 serine hydroxymethyltransferase -
  CFTSP3_RS05880 (CFTSP3_1182) lysS 1187353..1188864 (-) 1512 WP_231946250.1 lysine--tRNA ligase -
  CFTSP3_RS05885 (CFTSP3_1183) - 1188878..1189342 (-) 465 WP_023385424.1 Fur family transcriptional regulator -
  CFTSP3_RS05890 (CFTSP3_1184) - 1189339..1189917 (-) 579 WP_039362600.1 CvpA family protein -
  CFTSP3_RS05895 (CFTSP3_1185) pilT 1189917..1190978 (-) 1062 WP_023385428.1 type IV pilus twitching motility protein PilT Machinery gene
  CFTSP3_RS05900 (CFTSP3_1186) gatC 1190984..1191271 (-) 288 WP_058909390.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC -
  CFTSP3_RS09165 - 1191443..1191715 (+) 273 WP_144428493.1 hypothetical protein -
  CFTSP3_RS09170 - 1191857..1192039 (+) 183 WP_058909392.1 hypothetical protein -
  CFTSP3_RS05915 (CFTSP3_1188) - 1192042..1193052 (+) 1011 WP_058909393.1 hypothetical protein -
  CFTSP3_RS05920 (CFTSP3_1189) - 1193059..1193388 (+) 330 WP_023385435.1 hypothetical protein -
  CFTSP3_RS05925 (CFTSP3_1190) - 1193375..1194007 (+) 633 WP_023385437.1 type III pantothenate kinase -
  CFTSP3_RS05930 (CFTSP3_1191) hisG 1193995..1194606 (+) 612 WP_023385439.1 ATP phosphoribosyltransferase -
  CFTSP3_RS05935 (CFTSP3_1192) brnQ 1194615..1195919 (+) 1305 WP_023385441.1 branched-chain amino acid transport system II carrier protein -

Sequence

Protein


Download         Length: 353 a.a.        Molecular weight: 39105.67 Da        Isoelectric Point: 6.5919

>NTDB_id=140871 CFTSP3_RS05895 WP_023385428.1 1189917..1190978(-) (pilT) [Campylobacter fetus subsp. testudinum Sp3]
MNIETLLKTVMHNKASDLHLVSRSEPQIRIDGSLRPLEGKVLDGSDIENLCYVILTDAQKSELEENRDLDFALELPGIGR
FRGNCYYTMNGSLAAAFRMIPLNVPSLDDLSAPKIFKELVKKEKGLVLVTGGTGAGKSTTLAALLNEINLTERKHIITIE
DPVEFVHQNKNSLFSHRNVGTDTKSFARALKYALREDPDIILVGDLRDPETISIAIAAAETGHLVFGTLHTNSSVQTINR
IVDSFNETEQAQVRNMLSLSLNAIISQTLVPKADNGRFAIYEILINNHAISNLIRENKSHQIYSQIQLNQQNTGMQTQTQ
AMEKAVKMGIITKENAIRYSTNQQELAGKLGVI

Nucleotide


Download         Length: 1062 bp        

>NTDB_id=140871 CFTSP3_RS05895 WP_023385428.1 1189917..1190978(-) (pilT) [Campylobacter fetus subsp. testudinum Sp3]
ATGAATATCGAAACTCTTTTAAAAACCGTGATGCATAACAAGGCGAGCGATTTGCATTTAGTAAGTAGAAGTGAGCCTCA
AATCAGAATTGATGGTAGCTTAAGACCGCTTGAAGGTAAAGTTTTAGATGGAAGTGATATAGAAAATTTATGCTATGTTA
TTTTAACTGATGCTCAAAAAAGCGAATTAGAAGAAAATAGGGATTTAGACTTTGCACTCGAACTACCAGGAATAGGGCGT
TTTAGAGGTAATTGCTACTATACTATGAACGGAAGTTTAGCAGCAGCTTTTAGAATGATACCTTTAAATGTGCCAAGTCT
TGATGATCTCAGTGCTCCAAAGATTTTTAAAGAGTTGGTAAAGAAAGAAAAAGGTCTTGTGCTTGTTACTGGTGGAACTG
GAGCTGGAAAATCAACAACTCTAGCAGCTTTGCTAAATGAGATAAATCTTACTGAGAGAAAGCATATCATCACTATAGAA
GATCCTGTAGAGTTTGTTCATCAAAATAAAAATTCTCTATTTTCCCATAGAAACGTCGGTACAGATACTAAATCATTTGC
TAGAGCTTTAAAGTACGCACTTAGAGAAGATCCAGATATCATATTAGTTGGTGATTTAAGAGATCCAGAAACCATATCTA
TAGCTATAGCCGCTGCAGAAACAGGGCATTTGGTATTTGGTACTTTACACACGAACTCATCAGTGCAGACGATAAATAGA
ATAGTTGATAGTTTTAATGAGACCGAACAAGCTCAAGTAAGAAATATGCTTAGCTTATCTCTAAATGCTATCATATCCCA
AACTCTCGTGCCAAAAGCAGATAATGGTAGATTTGCTATCTATGAAATTTTGATAAATAATCATGCGATTTCAAATTTGA
TCAGAGAAAATAAATCTCATCAAATTTACTCCCAAATACAATTAAATCAACAAAATACTGGTATGCAAACACAAACTCAA
GCAATGGAAAAGGCGGTAAAAATGGGCATTATAACAAAAGAAAATGCTATAAGATATTCGACAAATCAGCAAGAATTAGC
TGGAAAGTTAGGTGTAATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria gonorrhoeae MS11

50.287

98.584

0.496
  pilT Neisseria meningitidis 8013

50

98.584

0.493
  pilT Legionella pneumophila strain Lp02

50.146

97.167

0.487
  pilT Legionella pneumophila strain ERS1305867

50.146

97.167

0.487
  pilT Acinetobacter baumannii strain A118

49.275

97.734

0.482
  pilT Acinetobacter baumannii D1279779

49.275

97.734

0.482
  pilT Acinetobacter nosocomialis M2

49.275

97.734

0.482
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.057

93.768

0.479
  pilT Vibrio cholerae strain A1552

51.057

93.768

0.479
  pilT Pseudomonas aeruginosa PAK

49.405

95.184

0.47
  pilT Acinetobacter baylyi ADP1

50.151

93.768

0.47
  pilT Pseudomonas stutzeri DSM 10701

48.81

95.184

0.465
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.667

98.584

0.411
  pilU Pseudomonas stutzeri DSM 10701

38.983

100

0.391
  pilU Acinetobacter baylyi ADP1

38.304

96.884

0.371

Multiple sequence alignment