Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QR90_RS14685 Genome accession   NZ_CP010028
Coordinates   3138601..3139848 (+) Length   415 a.a.
NCBI ID   WP_039685663.1    Uniprot ID   A0A0A7KLA9
Organism   Deinococcus radiopugnans strain DY59     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3133601..3144848
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QR90_RS14670 (QR90_14670) pgeF 3134481..3135236 (+) 756 WP_039685656.1 peptidoglycan editing factor PgeF -
  QR90_RS14675 (QR90_14675) - 3135233..3135739 (+) 507 WP_039685659.1 YqeG family HAD IIIA-type phosphatase -
  QR90_RS14680 (QR90_14680) pilB 3135847..3138525 (+) 2679 WP_039685661.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  QR90_RS14685 (QR90_14685) pilT 3138601..3139848 (+) 1248 WP_039685663.1 type IV pilus twitching motility protein PilT Machinery gene
  QR90_RS14690 (QR90_14690) lon 3139918..3142353 (-) 2436 WP_039685665.1 endopeptidase La -
  QR90_RS14695 (QR90_14695) clpX 3142502..3143716 (-) 1215 WP_039685667.1 ATP-dependent Clp protease ATP-binding subunit ClpX -
  QR90_RS14700 (QR90_14700) clpP 3143713..3144318 (-) 606 WP_039685669.1 ATP-dependent Clp protease proteolytic subunit -
  QR90_RS14705 (QR90_14705) - 3144373..3144726 (-) 354 WP_039685672.1 hypothetical protein -

Sequence

Protein


Download         Length: 415 a.a.        Molecular weight: 44879.26 Da        Isoelectric Point: 8.0872

>NTDB_id=133680 QR90_RS14685 WP_039685663.1 3138601..3139848(+) (pilT) [Deinococcus radiopugnans strain DY59]
MTQPSADITDILRIAAEKGASDVILTVGMAPQFKIDGVYGPQGFADLVPTETRKLMYSMMNEKQQRTFEERRELDFSFAL
GDRARFRVNCFMQRGNVGGVMRLIPTKIKSAAEMGLPQTVVDIAGAPRGLVLVTGPTGSGKSTTLAAMLDHINSTKKMHI
MTIEDPIEFMHAHKQSIINQREVGSDTMSFENALRAVLRQAPDVILVGEMRDHETIKAAVTAAETGHLVMGTLHTNSAPE
SIDRIVDVFPEEQQEQIRVQLANNLVAVMTQQLLPRLDGQGRILAYELLIANPAVRALIREGKTYQITSVMQTGAREGMV
TMDAFLANLYRRRMISFDVGASRAVDPKEFARLANDAGGAAAGNQPQANYTPSPASSPAAGSTKATGWGETGRAAATTTP
ETGYGGPASYGRGKR

Nucleotide


Download         Length: 1248 bp        

>NTDB_id=133680 QR90_RS14685 WP_039685663.1 3138601..3139848(+) (pilT) [Deinococcus radiopugnans strain DY59]
ATGACCCAGCCCTCCGCCGACATCACCGACATCCTGCGAATCGCCGCCGAGAAGGGCGCGTCCGACGTGATCCTGACCGT
GGGCATGGCCCCGCAGTTCAAGATCGACGGCGTGTACGGCCCGCAGGGGTTCGCCGATCTGGTGCCCACCGAGACGCGCA
AGCTGATGTACTCGATGATGAACGAGAAGCAGCAGCGCACCTTCGAGGAACGCCGCGAGCTGGACTTCAGCTTTGCCCTG
GGGGACCGTGCCCGCTTCCGCGTGAACTGCTTTATGCAGCGCGGCAACGTGGGCGGCGTGATGCGATTGATCCCTACCAA
GATCAAGAGCGCCGCCGAGATGGGCCTGCCGCAGACGGTTGTCGATATCGCAGGCGCGCCGCGCGGACTGGTGCTGGTCA
CCGGGCCGACCGGATCGGGCAAGAGCACCACGCTGGCGGCCATGCTTGACCACATCAATTCCACAAAGAAGATGCACATC
ATGACCATCGAGGACCCCATCGAGTTCATGCACGCGCACAAGCAGAGCATCATCAACCAGCGCGAGGTCGGCTCCGACAC
CATGAGCTTCGAGAACGCCCTGCGCGCGGTGCTGCGTCAGGCCCCCGACGTGATTCTGGTGGGCGAAATGCGCGACCACG
AGACGATCAAGGCCGCCGTCACCGCCGCCGAGACCGGGCACCTGGTGATGGGCACGCTGCACACCAACAGCGCGCCGGAA
TCGATTGACCGCATCGTGGACGTGTTCCCCGAAGAGCAGCAGGAGCAGATCCGGGTGCAGCTCGCCAACAACCTGGTGGC
CGTGATGACCCAGCAGCTGCTGCCACGCCTGGACGGGCAGGGCCGCATCCTGGCCTACGAACTGCTGATCGCCAACCCCG
CCGTGCGCGCCCTGATCCGCGAGGGCAAGACGTATCAGATCACCAGCGTCATGCAGACCGGCGCACGCGAGGGCATGGTC
ACCATGGACGCCTTTCTGGCGAACCTGTACCGCCGCCGCATGATCAGCTTCGACGTGGGGGCCTCGCGCGCGGTCGATCC
CAAGGAATTTGCACGGCTGGCCAACGACGCCGGGGGGGCCGCCGCCGGAAATCAGCCGCAGGCCAATTACACGCCTTCGC
CGGCCAGTTCTCCAGCTGCGGGGTCCACCAAGGCCACCGGCTGGGGGGAAACGGGGCGGGCGGCAGCCACCACCACCCCC
GAAACAGGCTACGGCGGCCCCGCCAGCTACGGCCGGGGCAAACGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(15-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0A7KLA9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

78.036

93.253

0.728
  pilT Acinetobacter nosocomialis M2

51.163

82.892

0.424
  pilT Acinetobacter baumannii D1279779

51.163

82.892

0.424
  pilT Acinetobacter baumannii strain A118

51.163

82.892

0.424
  pilT Neisseria meningitidis 8013

51.613

82.169

0.424
  pilT Acinetobacter baylyi ADP1

50.581

82.892

0.419
  pilT Pseudomonas stutzeri DSM 10701

50.581

82.892

0.419
  pilT Neisseria gonorrhoeae MS11

51.039

81.205

0.414
  pilT Pseudomonas aeruginosa PAK

50

82.892

0.414
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

47.826

83.133

0.398
  pilT Vibrio cholerae strain A1552

47.826

83.133

0.398
  pilT Legionella pneumophila strain Lp02

47.384

82.892

0.393
  pilT Legionella pneumophila strain ERS1305867

47.384

82.892

0.393
  pilU Pseudomonas stutzeri DSM 10701

42.614

84.819

0.361

Multiple sequence alignment