Detailed information NTG0133679

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: QR90_RS14680
• Genome accession: NZ_CP010028
• Coordinates: 3135847..3138525 (+)
• Length: 892 a.a.
• NCBI ID: WP_039685661.1
• Uniprot ID: A0A0A7KN08
• Organism: Deinococcus radiopugnans strain DY59
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3130847..3143525

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QR90_RS14645 (QR90_14645)	-	3130859..3131290 (+)	432	WP_039685651.1	hypothetical protein	-
QR90_RS14655 (QR90_14655)	-	3131496..3131918 (-)	423	WP_052195536.1	HIT family protein	-
QR90_RS14660 (QR90_14660)	guaB	3131970..3133487 (-)	1518	WP_039685653.1	IMP dehydrogenase	-
QR90_RS14665 (QR90_14665)	-	3133575..3134360 (-)	786	WP_039685655.1	enoyl-ACP reductase FabI	-
QR90_RS14670 (QR90_14670)	pgeF	3134481..3135236 (+)	756	WP_039685656.1	peptidoglycan editing factor PgeF	-
QR90_RS14675 (QR90_14675)	-	3135233..3135739 (+)	507	WP_039685659.1	YqeG family HAD IIIA-type phosphatase	-
QR90_RS14680 (QR90_14680)	pilB	3135847..3138525 (+)	2679	WP_039685661.1	ATPase, T2SS/T4P/T4SS family	Machinery gene
QR90_RS14685 (QR90_14685)	pilT	3138601..3139848 (+)	1248	WP_039685663.1	type IV pilus twitching motility protein PilT	Machinery gene
QR90_RS14690 (QR90_14690)	lon	3139918..3142353 (-)	2436	WP_039685665.1	endopeptidase La	-

Sequence

Protein

• Length: 892 a.a.
• Molecular weight: 97134.20 Da
• Isoelectric Point: 4.8193

>NTDB_id=133679 QR90_RS14680 WP_039685661.1 3135847..3138525(+) (pilB) [Deinococcus radiopugnans strain DY59]
MALSIGDRRLGAILLEQGYVNDGDLQKALVRHSEVGGRLAEILIDTGLVGEKRIARAIEEALGIPLVNLMAITPDPVAVA
AIPAQTALNLQAIPFALENGTLRVALVDPLSSLALETLEDDSNLTIEPYQALREQALWAVALHYPELGLTPVTPDEAGEE
VAGGQLGQALITRGLITDAQLQVALDAQQQTGEPLGTTLVAQQAISEDQLYELLAEQSDVIYLKNPRDFQPAEDVLGSML
RADALRLSAVPVDETAEHVVVVATDPRKREDIAALVGRPIQLILARPHDLEVLIERFYPQRGRLGEQMVQQGTLSRTQLR
EALQVQARQGKVKPLGEVILELGFADASEIDNALQKQNVGGGRLEDTLVQSGKLSPEMLARSLAAQLGYEFLDPVQNPPD
AKVALLIPEPTARRYGVVPVRLQGEALVIAMKDPRNVFALDDLKLITGREIIPAVMAEKDIVRLIERYFGNQGMADLNQR
LQAESLQREKDSKRGGDVDANANFDDNAVVKVVDGLIREAALQEASDIHIEPTESALRVRYRVDGVLREQNELPKGAAQS
ILARIKIMGNLDISERRIPQDGRVRFRKGSIDIDLRLSTLPTVYGEKAVMRLLQKASNIPEVEQLGFSDHNFQRYLDVID
KPNGIFLVTGPTGSGKSFTSFSTLKRIAKPEKNTTTIEDPVEYEIPGIIQSQVNPVAGLTFARALRAFLRQDPDIIFVGE
IRDTETAKIAVEAALTGHLVLATLHTNDAPGAIVRLEEMGVEPFNISASVVGVLAQRLVRRVCPDCKAPTNADPDVLRRL
GITERDVRGATLMRGAGCARCGGTGYKGRMGIHELMVVDEPLRVAIGSGRSATEIRDVALERSDMRTLRNDGIEKALKGQ
TTLEEVLAVTSN

Nucleotide

• Length: 2679 bp

>NTDB_id=133679 QR90_RS14680 WP_039685661.1 3135847..3138525(+) (pilB) [Deinococcus radiopugnans strain DY59]
ATGGCACTTTCTATCGGTGACCGCCGACTGGGCGCGATTCTGCTCGAACAGGGGTACGTCAACGACGGCGACCTGCAAAA
GGCGCTGGTGCGTCACTCTGAAGTCGGCGGACGGCTGGCCGAGATCCTGATCGATACCGGCCTGGTGGGCGAAAAGCGCA
TTGCGCGGGCCATCGAGGAGGCGCTGGGCATCCCGCTGGTCAACCTGATGGCGATCACGCCGGACCCGGTGGCGGTGGCG
GCGATTCCGGCCCAGACCGCGCTGAACCTCCAGGCCATTCCCTTTGCGCTGGAAAACGGCACCCTGCGCGTGGCGCTGGT
GGACCCCCTGTCCAGCCTGGCGCTGGAAACGCTGGAAGACGACAGCAACCTGACCATCGAGCCGTATCAGGCGCTGCGCG
AGCAGGCGCTGTGGGCCGTGGCGCTGCACTACCCGGAACTGGGTCTGACGCCGGTCACGCCCGACGAGGCCGGCGAGGAG
GTGGCGGGCGGCCAGCTGGGTCAGGCGCTGATCACGCGCGGCCTGATCACGGACGCGCAGCTGCAGGTGGCCCTGGACGC
CCAGCAGCAGACCGGCGAGCCGCTGGGGACCACCCTGGTGGCGCAGCAGGCCATCAGCGAGGACCAGCTCTACGAATTGC
TGGCCGAGCAGAGCGACGTCATCTACCTGAAAAACCCGCGTGACTTCCAGCCGGCCGAGGACGTGCTGGGCAGCATGCTG
CGCGCCGACGCGCTGCGGCTCTCGGCCGTGCCGGTCGACGAGACCGCCGAGCATGTGGTGGTGGTGGCCACCGATCCGCG
CAAACGCGAGGACATCGCCGCGCTGGTCGGGCGGCCCATCCAGCTGATCCTGGCGCGGCCGCACGACCTGGAAGTGCTGA
TCGAGCGCTTCTACCCGCAGCGCGGCCGGCTGGGCGAGCAGATGGTCCAGCAGGGCACGCTGTCGCGCACGCAGCTGCGC
GAGGCGCTGCAGGTCCAGGCCCGCCAGGGCAAGGTCAAGCCGCTGGGCGAGGTGATCCTGGAACTGGGCTTCGCCGACGC
CAGCGAGATTGACAACGCCCTGCAAAAGCAGAACGTGGGCGGCGGCCGCCTGGAAGACACGCTGGTGCAGTCGGGCAAGC
TGTCGCCGGAGATGCTGGCCCGCTCGCTGGCCGCGCAGCTGGGCTACGAATTCCTCGATCCGGTCCAGAACCCGCCGGAC
GCCAAGGTGGCGTTGCTGATTCCCGAGCCCACCGCGCGGCGCTACGGCGTGGTGCCGGTGAGGTTGCAGGGCGAGGCGCT
GGTCATCGCCATGAAAGACCCGCGCAACGTCTTTGCGCTGGACGATCTCAAGCTGATCACCGGGCGCGAGATCATCCCCG
CCGTGATGGCCGAGAAGGACATCGTGCGCCTGATCGAGCGCTACTTCGGCAATCAGGGCATGGCGGACCTGAACCAGCGC
CTGCAGGCCGAGTCCCTGCAGCGCGAGAAGGATTCCAAGCGCGGCGGCGACGTCGACGCAAACGCCAACTTCGACGACAA
CGCCGTCGTGAAGGTGGTGGACGGCCTGATCCGCGAGGCGGCCCTGCAGGAGGCCAGCGACATTCACATCGAGCCGACCG
AATCCGCTCTGCGCGTGCGCTACCGGGTGGACGGCGTGCTGCGCGAGCAGAACGAGCTGCCCAAGGGCGCGGCCCAGAGC
ATCCTGGCCCGCATCAAGATCATGGGCAACCTCGACATCTCCGAGCGCCGCATTCCGCAGGACGGGCGCGTGCGCTTCCG
CAAGGGCAGCATCGACATCGACCTGCGCCTGTCCACGTTGCCCACGGTGTACGGCGAGAAGGCCGTGATGCGCCTGCTGC
AAAAAGCCAGCAACATCCCCGAAGTCGAGCAACTGGGCTTTTCCGACCACAACTTCCAGCGCTACCTGGACGTGATCGAC
AAGCCCAACGGCATTTTCCTGGTCACCGGCCCTACCGGTTCGGGCAAGTCCTTCACCAGCTTTTCCACCCTCAAGCGCAT
CGCCAAGCCCGAGAAGAACACCACCACCATCGAGGACCCGGTGGAGTACGAGATTCCCGGCATCATCCAGTCGCAGGTCA
ACCCGGTGGCGGGCCTGACCTTTGCCCGCGCGCTGAGGGCCTTCCTGCGCCAGGACCCCGACATCATCTTCGTGGGGGAA
ATCCGGGACACCGAGACGGCCAAGATCGCCGTGGAAGCGGCCCTGACCGGGCACCTGGTGCTGGCGACGCTGCACACCAA
CGACGCGCCCGGCGCCATCGTGCGCCTGGAGGAGATGGGCGTGGAGCCGTTCAACATCAGCGCCTCGGTGGTGGGCGTGC
TGGCGCAGCGGCTGGTCCGCCGGGTCTGCCCCGACTGCAAGGCCCCCACCAACGCCGACCCGGACGTGCTGCGCCGCCTG
GGCATCACCGAGCGCGACGTGCGCGGGGCCACCCTGATGCGCGGCGCGGGCTGCGCGCGCTGCGGCGGCACCGGCTACAA
GGGCCGCATGGGCATCCATGAGCTGATGGTGGTGGACGAGCCGCTGCGCGTCGCCATCGGCTCGGGCCGGTCGGCCACGG
AGATCCGCGACGTCGCCCTAGAACGCAGCGACATGCGGACCCTGCGCAACGACGGCATCGAGAAGGCCCTCAAGGGCCAG
ACCACGCTGGAAGAGGTGCTGGCGGTCACCAGCAACTAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (517-781)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (368-473)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (43-145)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (199-302)

3D structure

• AlphaFold DB: A0A0A7KN08

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	76.484	100	0.766
pilF	Thermus thermophilus HB27	56.313	100	0.565