Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   U9975_RS01410 Genome accession   NZ_OY986432
Coordinates   324216..325295 (-) Length   359 a.a.
NCBI ID   WP_197166643.1    Uniprot ID   A0A7T1HLN2
Organism   Synechococcus sp. CBW1107 isolate KAC_105     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 319216..330295
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U9975_RS01395 (BBFGKLBO_00282) dusA 320495..321505 (-) 1011 WP_197166641.1 tRNA dihydrouridine(20/20a) synthase DusA -
  U9975_RS01400 (BBFGKLBO_00283) - 321512..322837 (+) 1326 WP_197166642.1 NAD(P)/FAD-dependent oxidoreductase -
  U9975_RS01405 (BBFGKLBO_00284) - 322802..324205 (-) 1404 WP_322782095.1 type II secretion system F family protein -
  U9975_RS01410 (BBFGKLBO_00285) pilT 324216..325295 (-) 1080 WP_197166643.1 type IV pilus twitching motility protein PilT Machinery gene
  U9975_RS01415 (BBFGKLBO_00286) - 325352..327160 (-) 1809 WP_322782096.1 GspE/PulE family protein -
  U9975_RS01420 (BBFGKLBO_00287) grpE 327258..327995 (+) 738 WP_322782097.1 nucleotide exchange factor GrpE -
  U9975_RS01425 (BBFGKLBO_00288) dnaJ 327995..329110 (+) 1116 WP_197166646.1 molecular chaperone DnaJ -
  U9975_RS01430 (BBFGKLBO_00289) - 329126..329419 (+) 294 WP_322782098.1 sulfurtransferase TusA family protein -

Sequence

Protein


Download         Length: 359 a.a.        Molecular weight: 39793.16 Da        Isoelectric Point: 6.0511

>NTDB_id=1162492 U9975_RS01410 WP_197166643.1 324216..325295(-) (pilT) [Synechococcus sp. CBW1107 isolate KAC_105]
MELQIEELMEELVEQKGSDLHLSAGQQPYGRFNGELRPMREQALSEESCNRMIFSILTNSQRKQLEQTWELDCSYGLRGL
ARFRINVFSQRGTYAASMRALNSKVPTPQELGLPPVVVDITKRPSGLVLVTGPTGSGKSTTLASLLDHINHSRAEHIITI
EDPIEFAYINDKSLIHQRQLNEDTKSFSNALRAALREDPDVILVGEMRDLETIQLAITAAETGHLVFATLHTSSAAQTVD
RMVDVFPAGQQAQIRTQLSSSLAAIFAQTLCRRSSPSNHNYGRVMAQEIMINTPALANLIREAKTAQIYSQIQTGGQLGM
QTLEKALADMVRQGDITLEEARTHSTKPDELNRLLGNSL

Nucleotide


Download         Length: 1080 bp        

>NTDB_id=1162492 U9975_RS01410 WP_197166643.1 324216..325295(-) (pilT) [Synechococcus sp. CBW1107 isolate KAC_105]
ATGGAGCTCCAGATCGAGGAGTTGATGGAAGAGCTGGTCGAGCAGAAGGGCAGCGACCTTCATCTCTCGGCCGGCCAGCA
ACCCTACGGACGCTTCAACGGGGAACTCAGGCCGATGCGGGAGCAGGCACTCAGCGAGGAATCCTGCAACCGCATGATCT
TCTCGATCCTCACCAACTCCCAGCGGAAGCAGCTGGAGCAGACCTGGGAACTCGATTGCTCCTACGGCCTGCGCGGCCTG
GCCCGCTTCCGCATCAATGTCTTCAGTCAGCGCGGCACCTACGCCGCGTCCATGCGGGCCCTGAACAGCAAGGTCCCCAC
TCCCCAGGAGCTCGGCCTGCCTCCGGTGGTGGTGGACATCACCAAGAGGCCTTCGGGACTTGTTCTGGTGACGGGTCCGA
CAGGATCGGGCAAGAGCACCACTCTGGCCTCCCTGCTCGATCACATCAACCACAGCCGTGCCGAGCACATCATCACGATC
GAAGATCCAATCGAGTTTGCTTACATCAATGACAAGAGCCTGATCCATCAGCGCCAGCTCAATGAAGACACCAAAAGCTT
CAGCAATGCTCTGAGAGCAGCGCTGCGTGAGGATCCCGATGTGATTCTGGTGGGGGAAATGCGCGATCTGGAAACGATCC
AGCTGGCCATCACCGCAGCCGAAACCGGCCACCTGGTGTTTGCCACCCTGCACACCAGTTCGGCCGCCCAGACCGTGGAT
CGCATGGTGGATGTGTTCCCGGCGGGCCAGCAGGCCCAGATCCGCACCCAGCTCAGCAGCAGCCTGGCGGCGATCTTCGC
CCAGACACTCTGCCGGAGAAGTTCACCCTCCAACCACAACTATGGCCGGGTGATGGCTCAGGAGATCATGATCAACACAC
CGGCCCTGGCCAATCTGATCCGCGAAGCGAAAACAGCCCAGATCTATTCCCAGATTCAGACCGGCGGACAGCTGGGCATG
CAGACCCTGGAGAAAGCCCTGGCGGACATGGTCAGGCAGGGAGACATCACCCTGGAGGAAGCCCGCACCCACTCCACCAA
GCCGGATGAGCTGAACCGTCTGCTCGGCAACTCCCTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7T1HLN2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

52.436

97.214

0.51
  pilT Acinetobacter baylyi ADP1

51.862

97.214

0.504
  pilT Pseudomonas stutzeri DSM 10701

51.576

97.214

0.501
  pilT Acinetobacter baumannii D1279779

51.289

97.214

0.499
  pilT Acinetobacter nosocomialis M2

51.289

97.214

0.499
  pilT Acinetobacter baumannii strain A118

51.289

97.214

0.499
  pilT Neisseria meningitidis 8013

51.471

94.708

0.487
  pilT Legionella pneumophila strain ERS1305867

49.575

98.329

0.487
  pilT Legionella pneumophila strain Lp02

49.575

98.329

0.487
  pilT Neisseria gonorrhoeae MS11

51.176

94.708

0.485
  pilT Vibrio cholerae strain A1552

50.147

94.986

0.476
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.147

94.986

0.476
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.011

98.05

0.471
  pilU Pseudomonas stutzeri DSM 10701

37.677

98.329

0.37
  pilU Acinetobacter baylyi ADP1

37.784

98.05

0.37

Multiple sequence alignment