Detailed information NTG1161859

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: ACKPCU_RS17125
• Genome accession: NZ_OY781932
• Coordinates: 3667798..3668199 (-)
• Length: 133 a.a.
• NCBI ID: WP_321348573.1
• Uniprot ID: -
• Organism: MAG: Halopseudomonas oceani isolate ab883223-6a80-4053-bf73-fb395571e785
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3662798..3673199

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACKPCU_RS17105 (U2971_RS17050)	-	3662877..3663677 (-)	801	WP_321348568.1	class I SAM-dependent methyltransferase	-
ACKPCU_RS17110 (U2971_RS17055)	-	3663670..3664860 (-)	1191	WP_321351873.1	oligosaccharide flippase family protein	-
ACKPCU_RS17115 (U2971_RS17060)	-	3664935..3665786 (-)	852	WP_321348570.1	glycosyltransferase family 2 protein	-
ACKPCU_RS17120 (U2971_RS17065)	-	3665783..3667717 (-)	1935	WP_321348571.1	hypothetical protein	-
ACKPCU_RS17125 (U2971_RS17070)	pilA	3667798..3668199 (-)	402	WP_321348573.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	Machinery gene
ACKPCU_RS17130 (U2971_RS17075)	pilB	3668498..3670198 (+)	1701	WP_321348577.1	type IV-A pilus assembly ATPase PilB	Machinery gene
ACKPCU_RS17135 (U2971_RS17080)	pilC	3670202..3671461 (+)	1260	WP_321348579.1	type II secretion system F family protein	Machinery gene
ACKPCU_RS17140 (U2971_RS17085)	pilD	3671465..3672322 (+)	858	WP_321348581.1	A24 family peptidase	Machinery gene
ACKPCU_RS17145 (U2971_RS17090)	coaE	3672358..3672960 (+)	603	WP_321348583.1	dephospho-CoA kinase	-
ACKPCU_RS17150 (U2971_RS17095)	yacG	3672957..3673148 (+)	192	WP_104737400.1	DNA gyrase inhibitor YacG	-

Sequence

Protein

• Length: 133 a.a.
• Molecular weight: 13781.88 Da
• Isoelectric Point: 6.1784

>NTDB_id=1161859 ACKPCU_RS17125 WP_321348573.1 3667798..3668199(-) (pilA) [MAG: Halopseudomonas oceani isolate ab883223-6a80-4053-bf73-fb395571e785]
MKAQKGFTLIELMIVVAIIGILAAIALPAYQDYTNRAKVSEIVLAASSARTCVTEIVQSDGFSSLDSCSTGFVATQYVSA
MTVAANTGVVTANGAGFGEAVTVTLKPSDTGPNRIGEWVCSGTPVKWMPGSCR

Nucleotide

• Length: 402 bp

>NTDB_id=1161859 ACKPCU_RS17125 WP_321348573.1 3667798..3668199(-) (pilA) [MAG: Halopseudomonas oceani isolate ab883223-6a80-4053-bf73-fb395571e785]
ATGAAAGCTCAAAAGGGTTTTACCTTGATCGAACTGATGATCGTTGTTGCGATCATCGGTATTCTGGCTGCTATCGCACT
GCCGGCTTATCAGGACTACACCAACCGCGCCAAGGTTTCTGAAATCGTTCTGGCTGCCAGCTCTGCTCGCACCTGCGTAA
CTGAAATCGTGCAGTCTGACGGCTTCTCCAGCCTGGATAGCTGCTCCACTGGCTTCGTTGCTACCCAGTACGTTTCTGCT
ATGACCGTTGCCGCAAATACTGGTGTTGTTACTGCAAATGGGGCTGGTTTTGGCGAGGCCGTAACTGTCACTCTTAAACC
GTCTGACACCGGTCCCAACCGTATTGGCGAGTGGGTTTGCTCTGGTACTCCGGTCAAGTGGATGCCGGGCTCTTGCCGTT
GA

Domains

Predicted by InterproScan.

• Pilin (bacterial filament) (Pilin) (36-133)

• Prokaryotic N-terminal methylation motif (N_methyl) (1-27)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Ralstonia pseudosolanacearum GMI1000	40.373	100	0.489
pilE	Neisseria gonorrhoeae strain FA1090	40.764	100	0.481
comP	Acinetobacter baylyi ADP1	42.568	100	0.474
pilA/pilAI	Pseudomonas stutzeri DSM 10701	42.759	100	0.466
pilA2	Legionella pneumophila str. Paris	44.444	100	0.451
pilA2	Legionella pneumophila strain ERS1305867	44.444	100	0.451
pilA/pilA1	Eikenella corrodens VA1	37.255	100	0.429
pilA	Acinetobacter baumannii strain A118	38.298	100	0.406
pilA/pilAII	Pseudomonas stutzeri DSM 10701	37.41	100	0.391
pilA	Pseudomonas aeruginosa PAK	46.296	81.203	0.376
pilE	Neisseria gonorrhoeae MS11	51.579	71.429	0.368
pilE	Neisseria elongata subsp. glycolytica ATCC 29315	63.158	57.143	0.361