Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACKPCU_RS17135 Genome accession   NZ_OY781932
Coordinates   3670202..3671461 (+) Length   419 a.a.
NCBI ID   WP_321348579.1    Uniprot ID   -
Organism   MAG: Halopseudomonas oceani isolate ab883223-6a80-4053-bf73-fb395571e785     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3665202..3676461
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKPCU_RS17120 (U2971_RS17065) - 3665783..3667717 (-) 1935 WP_321348571.1 hypothetical protein -
  ACKPCU_RS17125 (U2971_RS17070) pilA 3667798..3668199 (-) 402 WP_321348573.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  ACKPCU_RS17130 (U2971_RS17075) pilB 3668498..3670198 (+) 1701 WP_321348577.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACKPCU_RS17135 (U2971_RS17080) pilC 3670202..3671461 (+) 1260 WP_321348579.1 type II secretion system F family protein Machinery gene
  ACKPCU_RS17140 (U2971_RS17085) pilD 3671465..3672322 (+) 858 WP_321348581.1 A24 family peptidase Machinery gene
  ACKPCU_RS17145 (U2971_RS17090) coaE 3672358..3672960 (+) 603 WP_321348583.1 dephospho-CoA kinase -
  ACKPCU_RS17150 (U2971_RS17095) yacG 3672957..3673148 (+) 192 WP_104737400.1 DNA gyrase inhibitor YacG -
  ACKPCU_RS17155 (U2971_RS17100) - 3673151..3674098 (+) 948 WP_321348587.1 Nudix family hydrolase -
  ACKPCU_RS17160 (U2971_RS17105) ispH 3674149..3675093 (-) 945 WP_321348589.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  ACKPCU_RS17165 (U2971_RS17110) fkpB 3675142..3675573 (-) 432 WP_321348591.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  ACKPCU_RS17170 (U2971_RS17115) lspA 3675619..3676116 (-) 498 WP_321348593.1 signal peptidase II -

Sequence

Protein


Download         Length: 419 a.a.        Molecular weight: 46065.89 Da        Isoelectric Point: 9.5066

>NTDB_id=1161861 ACKPCU_RS17135 WP_321348579.1 3670202..3671461(+) (pilC) [MAG: Halopseudomonas oceani isolate ab883223-6a80-4053-bf73-fb395571e785]
MAQQAALKKNPARKAKKKEEKLYPFKWEGKDRKGTRIDGVLQGENAALVKTQLRKQGILVTKIKRESALFGKRPKPIKPL
DIAFFTRQLATMMESGVPIIQSFEIIAEGAENPSVGKLVTEIKTDVAAGNTLADSLRQHPKYFDDLFCNLVESGEQSGRL
ESLLDRIATYKEKTEALKAKIKKAMTYPIAVVVVAIIVTAIMLLKVVPSFQEVFANFGAELPAFTLFVIGLSEWLQSWWF
ILLAIIIATGYIYSQAHLRSKPFRDFEDRTLLKAPIVGDIVYQAAVARYARTLSTTFAAGVPLVDALDSVAGASGNVVFY
NAVMKIKEDVSAGSQLNFSMRATKVFPSLATQMTGIGEESGNLDGMLEKVADYYEAEVDNKVDNLTTLLEPMIMSVLGIL
VGGLIVAMYLPIFQLGNVV

Nucleotide


Download         Length: 1260 bp        

>NTDB_id=1161861 ACKPCU_RS17135 WP_321348579.1 3670202..3671461(+) (pilC) [MAG: Halopseudomonas oceani isolate ab883223-6a80-4053-bf73-fb395571e785]
ATGGCGCAACAAGCCGCATTGAAGAAGAATCCCGCCAGGAAGGCAAAGAAAAAAGAAGAGAAATTGTATCCCTTCAAATG
GGAGGGCAAGGATCGCAAGGGCACCAGAATCGATGGCGTCCTGCAGGGTGAAAATGCCGCGCTGGTGAAGACCCAGCTGC
GCAAACAGGGCATCCTGGTTACCAAAATCAAGCGCGAGTCGGCGCTGTTCGGAAAACGCCCCAAGCCGATCAAACCGCTG
GACATCGCTTTCTTCACTCGCCAGTTGGCGACCATGATGGAATCCGGCGTCCCCATTATCCAGTCCTTCGAAATTATCGC
CGAAGGCGCAGAAAACCCGAGCGTCGGCAAGCTGGTCACCGAGATCAAGACCGATGTTGCTGCCGGTAACACCTTGGCGG
ACTCTTTGCGACAGCACCCTAAATACTTCGACGATCTGTTCTGCAACCTGGTTGAGTCCGGCGAGCAATCTGGTCGACTG
GAGTCGCTGCTGGACCGGATCGCGACCTACAAAGAGAAAACAGAAGCTCTCAAAGCAAAAATCAAGAAGGCAATGACCTA
CCCGATCGCGGTAGTTGTGGTTGCGATCATCGTGACGGCCATCATGTTGCTGAAAGTAGTACCCAGCTTTCAGGAAGTTT
TTGCCAACTTCGGCGCCGAGTTACCCGCTTTCACGTTATTTGTGATCGGCCTATCTGAGTGGTTGCAGTCATGGTGGTTC
ATATTGCTCGCAATAATAATCGCGACTGGGTACATCTACTCACAAGCCCACCTTCGATCAAAACCCTTCCGGGACTTCGA
AGACCGCACACTTTTAAAAGCACCAATCGTTGGCGATATCGTTTATCAGGCTGCCGTGGCCCGTTACGCACGCACCCTGT
CCACGACCTTTGCTGCGGGTGTACCGCTGGTGGATGCACTGGATTCGGTCGCCGGTGCTTCCGGTAACGTGGTGTTCTAC
AACGCGGTAATGAAGATCAAAGAAGACGTGTCAGCTGGCTCACAGCTCAACTTCTCCATGCGCGCCACCAAAGTTTTCCC
CTCTCTGGCGACCCAGATGACCGGGATTGGCGAAGAGTCCGGTAACCTGGACGGCATGCTGGAAAAAGTCGCCGACTATT
ATGAGGCAGAGGTAGACAACAAGGTAGACAACCTGACCACTCTGCTGGAGCCGATGATCATGTCGGTGCTGGGTATTCTC
GTCGGCGGCCTGATTGTCGCCATGTACCTGCCGATCTTCCAGCTGGGTAACGTCGTTTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(85-208)

Type II secretion system (T2SS), protein F (T2SSF)

(290-411)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

68.861

94.272

0.649
  pilC Acinetobacter baylyi ADP1

58.968

97.136

0.573
  pilC Acinetobacter baumannii D1279779

58.722

97.136

0.57
  pilC Legionella pneumophila strain ERS1305867

51.733

96.42

0.499
  pilG Neisseria meningitidis 44/76-A

45.255

98.091

0.444
  pilG Neisseria gonorrhoeae MS11

45.012

98.091

0.442
  pilC Vibrio campbellii strain DS40M4

41.687

96.181

0.401
  pilC Vibrio cholerae strain A1552

41.439

96.181

0.399

Multiple sequence alignment