Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACKPCU_RS17130 Genome accession   NZ_OY781932
Coordinates   3668498..3670198 (+) Length   566 a.a.
NCBI ID   WP_321348577.1    Uniprot ID   -
Organism   MAG: Halopseudomonas oceani isolate ab883223-6a80-4053-bf73-fb395571e785     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3663498..3675198
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKPCU_RS17110 (U2971_RS17055) - 3663670..3664860 (-) 1191 WP_321351873.1 oligosaccharide flippase family protein -
  ACKPCU_RS17115 (U2971_RS17060) - 3664935..3665786 (-) 852 WP_321348570.1 glycosyltransferase family 2 protein -
  ACKPCU_RS17120 (U2971_RS17065) - 3665783..3667717 (-) 1935 WP_321348571.1 hypothetical protein -
  ACKPCU_RS17125 (U2971_RS17070) pilA 3667798..3668199 (-) 402 WP_321348573.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  ACKPCU_RS17130 (U2971_RS17075) pilB 3668498..3670198 (+) 1701 WP_321348577.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACKPCU_RS17135 (U2971_RS17080) pilC 3670202..3671461 (+) 1260 WP_321348579.1 type II secretion system F family protein Machinery gene
  ACKPCU_RS17140 (U2971_RS17085) pilD 3671465..3672322 (+) 858 WP_321348581.1 A24 family peptidase Machinery gene
  ACKPCU_RS17145 (U2971_RS17090) coaE 3672358..3672960 (+) 603 WP_321348583.1 dephospho-CoA kinase -
  ACKPCU_RS17150 (U2971_RS17095) yacG 3672957..3673148 (+) 192 WP_104737400.1 DNA gyrase inhibitor YacG -
  ACKPCU_RS17155 (U2971_RS17100) - 3673151..3674098 (+) 948 WP_321348587.1 Nudix family hydrolase -
  ACKPCU_RS17160 (U2971_RS17105) ispH 3674149..3675093 (-) 945 WP_321348589.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62345.59 Da        Isoelectric Point: 5.3444

>NTDB_id=1161860 ACKPCU_RS17130 WP_321348577.1 3668498..3670198(+) (pilB) [MAG: Halopseudomonas oceani isolate ab883223-6a80-4053-bf73-fb395571e785]
METPALSGLARRIVQEGLIEADVAAKACQQANQDKIPLITHLVQKKLADAKSLAMTCAEEFGYPYFDLGALDKEAQPKEL
VSEKLVRQHNVLPLFKRGSRLYLGISDPTNQQALSDVQFHTGLMTDAVIVEDDKLLNAIEKYLDSPTGGLGDMADADLDD
LDVQAVKDDNQSDGGSEADDAPIVRFVNKMLLDAVRMGSSDLHFEPYEKTYRVRFRTDGVLHEVAKPPVQLGVRIAARLK
VMSSMDLAERRKPQDGRIKLKISKNKAIDFRVNTLPTLWGEKVVLRILDSESAKMGIDALGYEEDQKQMYLAALARPQGM
ILVTGPTGSGKTVSLYTGLNILNTMERNISTAEDPVEINLEGINQVNVNPKQGLDFAQALRAFLRQDPDIIMVGEIRDLE
TAEIAIKAAQTGHMVMSTLHTNSAAETLTRMRNMGVPSFNIATSVNLIIAQRLARRLCKTCKTKMDVPREVLLEEGFTPE
KIDAGLTIYGPVGCENCKDGYKGRVGIYEVVKITPAMQRIIMEDGNSIQISDVAQSEGFRNLRQSALMKVTEGVTSLAEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=1161860 ACKPCU_RS17130 WP_321348577.1 3668498..3670198(+) (pilB) [MAG: Halopseudomonas oceani isolate ab883223-6a80-4053-bf73-fb395571e785]
ATGGAAACCCCCGCTCTTTCAGGGCTCGCACGACGAATCGTGCAGGAAGGCCTCATCGAGGCTGACGTGGCTGCCAAGGC
ATGCCAGCAGGCAAACCAGGACAAAATTCCGCTCATCACGCACCTAGTACAGAAAAAGCTAGCCGACGCCAAATCCCTCG
CCATGACCTGCGCCGAGGAGTTCGGCTACCCCTATTTCGACCTGGGTGCTCTGGACAAAGAGGCCCAGCCCAAAGAGTTG
GTCAGTGAAAAGCTGGTCCGTCAGCACAACGTTCTACCGCTATTCAAGCGCGGATCGCGCCTGTACCTCGGTATATCAGA
CCCGACCAACCAGCAGGCACTGAGTGACGTTCAGTTTCACACAGGCCTGATGACCGATGCCGTCATTGTCGAAGATGACA
AACTGCTCAACGCCATAGAGAAGTATCTGGACAGCCCGACCGGTGGATTGGGTGACATGGCCGATGCAGACCTTGATGAC
CTAGATGTACAAGCGGTTAAGGACGACAACCAGTCAGACGGCGGCTCCGAGGCAGACGACGCCCCCATTGTGCGCTTCGT
CAACAAAATGCTGCTCGACGCCGTCCGCATGGGCTCATCTGACTTGCACTTCGAACCCTATGAGAAGACATATCGAGTCC
GGTTCCGCACTGACGGGGTGTTGCACGAAGTCGCAAAACCGCCGGTACAGCTAGGCGTCCGGATTGCAGCGCGCCTGAAG
GTTATGTCGTCCATGGACCTCGCCGAGCGACGCAAGCCACAGGATGGCCGCATCAAGCTGAAAATATCCAAGAACAAGGC
AATCGACTTCCGGGTTAACACGCTGCCAACCCTATGGGGTGAAAAAGTGGTGCTACGTATCCTCGACTCGGAAAGCGCCA
AGATGGGCATCGACGCCTTGGGTTACGAGGAGGACCAGAAGCAGATGTATCTGGCAGCCTTGGCCAGACCGCAGGGCATG
ATTCTGGTGACCGGCCCGACCGGCTCAGGCAAAACCGTGTCGCTGTATACCGGCCTGAACATCCTCAACACCATGGAGCG
AAATATCTCCACCGCCGAGGATCCGGTTGAAATCAACCTTGAAGGCATTAACCAAGTCAACGTCAACCCCAAGCAGGGCC
TAGATTTTGCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCCGATATCATCATGGTGGGCGAGATCCGCGACCTGGAA
ACCGCCGAGATTGCAATCAAAGCCGCCCAGACCGGCCACATGGTTATGTCGACGCTGCACACCAACAGCGCAGCCGAAAC
CCTGACGCGCATGCGCAATATGGGCGTACCATCGTTCAATATTGCCACCTCCGTAAACCTGATCATCGCCCAGCGGTTGG
CGCGTCGCCTGTGCAAGACCTGTAAGACCAAGATGGATGTCCCTCGCGAAGTACTGCTTGAAGAGGGCTTCACTCCAGAA
AAAATTGATGCAGGCCTAACAATTTACGGGCCCGTGGGTTGCGAAAACTGCAAAGACGGTTATAAAGGACGTGTTGGTAT
CTATGAGGTCGTCAAGATCACCCCGGCGATGCAGCGCATCATCATGGAAGACGGCAACTCTATTCAGATCTCTGACGTAG
CCCAGAGTGAAGGTTTCAGAAACCTGCGCCAGTCCGCCCTTATGAAAGTAACAGAGGGTGTCACCAGCCTGGCGGAAGTC
AACCGAGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(189-456)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.541

100

0.595
  pilB Acinetobacter baylyi ADP1

59.043

99.647

0.588
  pilB Legionella pneumophila strain ERS1305867

56.36

100

0.564
  pilB Vibrio cholerae strain A1552

50.712

99.293

0.504
  pilF Neisseria gonorrhoeae MS11

49.291

99.647

0.491
  pilB Vibrio parahaemolyticus RIMD 2210633

49.466

99.293

0.491
  pilB Vibrio campbellii strain DS40M4

47.276

100

0.475
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.79

99.293

0.385
  pilF Thermus thermophilus HB27

36.602

100

0.369

Multiple sequence alignment