Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACKPCD_RS06095 Genome accession   NZ_OY759718
Coordinates   1250327..1251343 (+) Length   338 a.a.
NCBI ID   WP_321434361.1    Uniprot ID   -
Organism   MAG: Trichococcus flocculiformis isolate 659e37ff-3402-4023-a990-0c9469b16b9d     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1245327..1256343
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKPCD_RS06075 (U2959_RS06055) - 1246187..1246615 (+) 429 WP_321434357.1 hypothetical protein -
  ACKPCD_RS06080 (U2959_RS06060) - 1246560..1247051 (+) 492 WP_321434358.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ACKPCD_RS06085 (U2959_RS06065) - 1247060..1248670 (+) 1611 WP_321434359.1 PilX N-terminal domain-containing pilus assembly protein -
  ACKPCD_RS06090 (U2959_RS06070) - 1248688..1250313 (+) 1626 WP_321434360.1 ATPase, T2SS/T4P/T4SS family -
  ACKPCD_RS06095 (U2959_RS06075) pilT 1250327..1251343 (+) 1017 WP_321434361.1 type IV pilus twitching motility protein PilT Machinery gene
  ACKPCD_RS06100 (U2959_RS06080) - 1251383..1252591 (+) 1209 WP_321434362.1 type II secretion system F family protein -
  ACKPCD_RS06105 (U2959_RS06085) - 1252727..1253068 (+) 342 WP_321434363.1 type II secretion system protein -
  ACKPCD_RS06110 (U2959_RS06090) - 1253133..1253888 (+) 756 WP_321434364.1 A24 family peptidase -
  ACKPCD_RS06115 (U2959_RS06095) pilM 1253932..1254963 (+) 1032 WP_321434365.1 pilus assembly protein PilM -
  ACKPCD_RS06120 (U2959_RS06100) - 1254956..1255495 (+) 540 WP_321434366.1 hypothetical protein -
  ACKPCD_RS06125 (U2959_RS06105) - 1255492..1256052 (+) 561 WP_321434367.1 hypothetical protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37500.41 Da        Isoelectric Point: 6.8424

>NTDB_id=1161417 ACKPCD_RS06095 WP_321434361.1 1250327..1251343(+) (pilT) [MAG: Trichococcus flocculiformis isolate 659e37ff-3402-4023-a990-0c9469b16b9d]
MAQLDMILSVAYEKKASDVHLVVGAAPVFRVDGKLIPQKQAVLTPEDTEAFAKTILSQDLWETLEKKREIDFSYAINDIA
RFRVNAFYQRNALSLAFRIISKDIPSVESLGLPSIAKKLVQQPHGLFLVTGPTGSGKSTSLAALIDYVNETMNRHIITLE
DPIEYLHVHKQSIIEQREVGFDTLSFKDGLRASLRQDPDVILVGELRDMDTISTAITASETGHLVLGTLHTSDAAGSIER
MIDVFPAGQREQIRLMLANVLLGILSQRLLPTKKTNGRVAATEMLVNNAAVKNLIRAEKMHQIPNVLQTSMDQGMHTMEM
SIKRLVEAETISRSHLLL

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=1161417 ACKPCD_RS06095 WP_321434361.1 1250327..1251343(+) (pilT) [MAG: Trichococcus flocculiformis isolate 659e37ff-3402-4023-a990-0c9469b16b9d]
ATGGCACAATTGGATATGATATTGAGCGTTGCATATGAAAAAAAAGCTTCCGATGTCCACCTTGTCGTAGGCGCTGCGCC
GGTTTTTCGGGTGGACGGAAAGCTGATTCCCCAAAAGCAGGCAGTTCTTACGCCTGAAGATACAGAAGCGTTTGCCAAAA
CCATCCTGAGCCAGGACTTGTGGGAGACGCTGGAGAAAAAACGGGAAATTGATTTCTCCTATGCCATCAACGACATTGCC
CGTTTCCGGGTGAATGCCTTCTACCAACGCAACGCGCTTTCGCTTGCTTTTCGGATCATTTCCAAAGACATTCCCAGCGT
GGAAAGTTTAGGACTGCCCAGCATCGCTAAAAAATTGGTTCAACAGCCACATGGACTTTTCTTGGTTACCGGACCGACTG
GTAGCGGGAAAAGCACCTCACTGGCGGCGCTGATCGATTATGTGAATGAAACGATGAACCGGCACATCATCACATTGGAA
GATCCGATCGAATACTTGCATGTGCATAAGCAATCCATCATTGAACAGCGGGAAGTAGGCTTTGATACGTTGTCATTCAA
AGACGGCCTGCGCGCGAGTCTGCGCCAGGATCCCGATGTCATTCTGGTAGGGGAGCTGCGCGACATGGACACCATCTCCA
CGGCCATCACAGCCTCGGAAACGGGCCATTTGGTCTTGGGGACGCTGCACACTTCGGATGCGGCCGGCAGCATCGAGCGG
ATGATCGATGTCTTCCCGGCCGGGCAAAGAGAGCAGATCCGTTTGATGCTGGCGAATGTACTATTGGGCATCTTGTCGCA
GCGCTTGCTGCCGACCAAGAAGACCAATGGAAGGGTGGCCGCGACGGAAATGCTGGTCAATAATGCAGCGGTCAAAAATC
TGATCCGGGCAGAAAAAATGCATCAAATCCCGAATGTACTGCAGACCTCGATGGACCAAGGCATGCACACGATGGAGATG
AGCATCAAGCGGCTCGTTGAAGCAGAGACAATCAGTCGGTCGCATCTATTACTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.547

97.929

0.485
  pilT Neisseria meningitidis 8013

47.84

95.858

0.459
  pilT Neisseria gonorrhoeae MS11

47.531

95.858

0.456
  pilT Legionella pneumophila strain ERS1305867

46.483

96.746

0.45
  pilT Legionella pneumophila strain Lp02

46.483

96.746

0.45
  pilT Acinetobacter baumannii strain A118

46.319

96.45

0.447
  pilT Acinetobacter nosocomialis M2

46.319

96.45

0.447
  pilT Acinetobacter baumannii D1279779

46.319

96.45

0.447
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

46.875

94.675

0.444
  pilT Vibrio cholerae strain A1552

46.875

94.675

0.444
  pilT Pseudomonas aeruginosa PAK

44.985

97.337

0.438
  pilT Pseudomonas stutzeri DSM 10701

44.648

96.746

0.432
  pilT Acinetobacter baylyi ADP1

45.312

94.675

0.429
  pilU Pseudomonas stutzeri DSM 10701

39.286

99.408

0.391
  pilU Vibrio cholerae strain A1552

40

97.633

0.391

Multiple sequence alignment