Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   EAKF1_RS15910 Genome accession   NZ_CP007025
Coordinates   3194300..3195280 (+) Length   326 a.a.
NCBI ID   WP_010336949.1    Uniprot ID   -
Organism   Escherichia albertii KF1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3189300..3200280
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EAKF1_RS15880 (EAKF1_ch3035) - 3189906..3190913 (+) 1008 WP_025238393.1 DUF1202 family protein -
  EAKF1_RS15885 (EAKF1_ch3036c) hemW 3190978..3192114 (-) 1137 WP_000239934.1 radical SAM family heme chaperone HemW -
  EAKF1_RS15890 (EAKF1_ch3037c) - 3192118..3192699 (-) 582 WP_025238394.1 XTP/dITP diphosphatase -
  EAKF1_RS15895 (EAKF1_ch3038c) yggU 3192707..3192997 (-) 291 WP_001277225.1 DUF167 family protein YggU -
  EAKF1_RS15900 (EAKF1_ch3039c) yggT 3192994..3193560 (-) 567 WP_001094839.1 osmotic shock tolerance protein YggT -
  EAKF1_RS15905 (EAKF1_ch3040c) yggS 3193578..3194282 (-) 705 WP_025238395.1 pyridoxal phosphate homeostasis protein -
  EAKF1_RS15910 (EAKF1_ch3041) pilT 3194300..3195280 (+) 981 WP_010336949.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EAKF1_RS15915 (EAKF1_ch3042c) ruvX 3195464..3195880 (-) 417 WP_000017099.1 Holliday junction resolvase RuvX -
  EAKF1_RS15920 (EAKF1_ch3043c) - 3195880..3196443 (-) 564 WP_025238396.1 YqgE/AlgH family protein -
  EAKF1_RS15925 (EAKF1_ch3044c) gshB 3196552..3197502 (-) 951 WP_000593300.1 glutathione synthase -
  EAKF1_RS15930 (EAKF1_ch3045c) rsmE 3197515..3198246 (-) 732 WP_025238397.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  EAKF1_RS15935 (EAKF1_ch3046c) endA 3198326..3199033 (-) 708 WP_025238398.1 deoxyribonuclease I -
  EAKF1_RS15940 (EAKF1_ch3047c) - 3199128..3199625 (-) 498 WP_002461299.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36019.12 Da        Isoelectric Point: 5.5860

>NTDB_id=116027 EAKF1_RS15910 WP_010336949.1 3194300..3195280(+) (pilT) [Escherichia albertii KF1]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDTPDVEELLREWLDDDQRALLLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPTVLPELLNNENGLILVTGATGSGKSTTLAAMVGYLNQHTDAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=116027 EAKF1_RS15910 WP_010336949.1 3194300..3195280(+) (pilT) [Escherichia albertii KF1]
ATGAATATGGAAGAAATTGTGGCTCTTAGTGTAAAGCATAATGTCTCGGATCTACACCTGTGTAGCGCCTGGCCTGCACG
CTGGCGTATTCGCGGGAGGATGGAAGCTGCGCCGTTTGATACGCCGGACGTTGAAGAGCTGCTAAGGGAATGGCTGGATG
ACGATCAGCGAGCGTTATTGCTGGAGAATGGGCAGTTGGATTTTGCCGTGTCGTTGGCGGAAAACCAGCGATTGCGCGGC
AGTGCGTTCGCGCAACGGCAAGGCATTTCGCTGGCGTTACGGTTGTTGCCTTCGCATTGCCCGCAACTCGAACAGCTTGG
CGCGCCGACGGTATTACCGGAACTACTCAATAACGAGAATGGACTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CCACCACACTGGCGGCGATGGTTGGTTATCTCAATCAGCATACCGATGCGCATATTCTTACGCTGGAAGATCCTGTGGAA
TATCTTTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAGATTGGTTTGCACTGTATGACGTTTGCATCGGGATTACG
TGCGGCATTGCGGGAAGATCCCGATGTAATTTTGCTTGGCGAGCTGCGTGACAGCGAGACCATTCGTCTGGCGTTGACGG
CGGCAGAAACAGGTCATCTGGTGCTGGCAACCTTGCATACGCGCGGAGCGGCTCAGGCGGTCGAGCGGCTGGTGGATTCG
TTTCCGGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCGGGTAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTGCTGATTAACACACCTGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTGCCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACTTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.847

100

0.5
  pilT Vibrio cholerae strain A1552

49.847

100

0.5
  pilT Neisseria meningitidis 8013

47.866

100

0.482
  pilT Neisseria gonorrhoeae MS11

47.561

100

0.479
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Pseudomonas aeruginosa PAK

46.177

100

0.463
  pilT Legionella pneumophila strain ERS1305867

44.954

100

0.451
  pilT Legionella pneumophila strain Lp02

44.954

100

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

40.694

97.239

0.396
  pilU Pseudomonas stutzeri DSM 10701

38.554

100

0.393

Multiple sequence alignment