Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QM550_RS20915 Genome accession   NZ_OX442412
Coordinates   4268403..4269383 (+) Length   326 a.a.
NCBI ID   WP_020899201.1    Uniprot ID   V7IVS7
Organism   Salmonella enterica subsp. enterica serovar Rissen isolate pure strain     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4263403..4274383
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QM550_RS20885 - 4263980..4264987 (+) 1008 WP_023138752.1 DUF1202 domain-containing protein -
  QM550_RS20890 hemW 4265079..4266215 (-) 1137 WP_023138753.1 radical SAM family heme chaperone HemW -
  QM550_RS20895 - 4266208..4266801 (-) 594 WP_001174769.1 XTP/dITP diphosphatase -
  QM550_RS20900 yggU 4266809..4267099 (-) 291 WP_001277205.1 DUF167 family protein YggU -
  QM550_RS20905 - 4267096..4267662 (-) 567 WP_001094848.1 YggT family protein -
  QM550_RS20910 - 4267681..4268385 (-) 705 WP_000997790.1 YggS family pyridoxal phosphate-dependent enzyme -
  QM550_RS20915 pilT 4268403..4269383 (+) 981 WP_020899201.1 type IV pilus twitching motility protein PilT Machinery gene
  QM550_RS20920 - 4269513..4270199 (+) 687 WP_000098329.1 IclR family transcriptional regulator -
  QM550_RS20925 ruvX 4270246..4270662 (-) 417 WP_001285490.1 Holliday junction resolvase RuvX -
  QM550_RS20930 - 4270662..4271225 (-) 564 WP_001053167.1 YqgE/AlgH family protein -
  QM550_RS20935 gshB 4271441..4272388 (-) 948 WP_000593248.1 glutathione synthase -
  QM550_RS20940 rsmE 4272408..4273139 (-) 732 WP_001222488.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  QM550_RS20945 endA 4273216..4273923 (-) 708 WP_000286124.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36140.34 Da        Isoelectric Point: 7.5355

>NTDB_id=1158347 QM550_RS20915 WP_020899201.1 4268403..4269383(+) (pilT) [Salmonella enterica subsp. enterica serovar Rissen isolate pure strain]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMEIAPFTAPDVDRLLLDWLNDAQQYQWRTHGQLDFAVSLSGTRRLRA
SAFTHQQGTSLALRLLPERCPDLAEIQTPPIVPALLASENGLILVTGATGCGKSTTLAAMVGHLNQHADKHILTLEDPIE
YRYTSKRCLIQQREIGQHCATFAAGLRAALREDPDVILLGELRDSETIRLSLTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPVRSQLAGSLRAVLSQKLEVDRQDGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=1158347 QM550_RS20915 WP_020899201.1 4268403..4269383(+) (pilT) [Salmonella enterica subsp. enterica serovar Rissen isolate pure strain]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGCATGGAGATCGCGCCTTTTACCGCGCCTGATGTCGACAGGCTTCTGCTTGATTGGCTTAATG
ACGCGCAGCAATATCAGTGGCGAACGCACGGCCAGCTTGATTTCGCCGTCTCGCTGTCTGGCACGCGGCGCCTGCGCGCC
AGTGCGTTCACACATCAACAGGGAACGTCGCTGGCGCTACGGCTATTGCCCGAGCGCTGTCCTGATCTGGCGGAAATCCA
GACGCCGCCGATAGTACCCGCACTGCTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGCGCTACTGGCTGCGGGAAGT
CCACTACGCTGGCGGCGATGGTGGGACATCTTAATCAACATGCCGATAAACACATCCTGACGCTGGAAGATCCTATTGAG
TACCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAATCGGCCAGCATTGCGCCACTTTTGCCGCCGGGCTGCG
CGCGGCGTTACGTGAAGATCCTGATGTCATTTTGCTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGTCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGCGGTGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGGTACGCAGCCAACTGGCGGGTAGTCTGCGCGCCGTACTATCACAAAAACTGGAGGT
CGACAGACAGGATGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT
CAGGCGCAGGGACGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB V7IVS7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.318

100

0.485
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.318

100

0.485
  pilT Neisseria meningitidis 8013

46.951

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.646

100

0.469
  pilT Acinetobacter baumannii strain A118

44.648

100

0.448
  pilT Acinetobacter baumannii D1279779

44.648

100

0.448
  pilT Acinetobacter baylyi ADP1

44.648

100

0.448
  pilT Pseudomonas aeruginosa PAK

44.648

100

0.448
  pilT Pseudomonas stutzeri DSM 10701

44.343

100

0.445
  pilT Acinetobacter nosocomialis M2

44.343

100

0.445
  pilT Legionella pneumophila strain Lp02

44.34

97.546

0.433
  pilT Legionella pneumophila strain ERS1305867

44.34

97.546

0.433
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

38.245

97.853

0.374
  pilU Pseudomonas stutzeri DSM 10701

36.036

100

0.368

Multiple sequence alignment