Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   C163_RS24445 Genome accession   NZ_CP007012
Coordinates   5563162..5564160 (-) Length   332 a.a.
NCBI ID   WP_025340967.1    Uniprot ID   A0AAI8AXR7
Organism   Pseudomonas sp. FGI182     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5558162..5569160
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C163_RS24425 (C163_24835) argS 5559230..5560966 (+) 1737 WP_025340964.1 arginine--tRNA ligase -
  C163_RS24430 (C163_24840) - 5560968..5561666 (+) 699 WP_025340965.1 SPOR domain-containing protein -
  C163_RS24435 (C163_24845) - 5561813..5562232 (-) 420 WP_013974614.1 TM2 domain-containing protein -
  C163_RS24440 (C163_24850) - 5562399..5563034 (+) 636 WP_025340966.1 C40 family peptidase -
  C163_RS24445 (C163_24855) pilT 5563162..5564160 (-) 999 WP_025340967.1 type IV pilus twitching motility protein PilT Machinery gene
  C163_RS24450 (C163_24860) - 5564226..5564912 (+) 687 WP_025340968.1 YggS family pyridoxal phosphate-dependent enzyme -
  C163_RS24455 (C163_24865) proC 5564951..5565769 (+) 819 WP_025340969.1 pyrroline-5-carboxylate reductase -
  C163_RS24460 (C163_24870) - 5565780..5566370 (+) 591 WP_013974619.1 YggT family protein -
  C163_RS24465 (C163_24875) - 5566470..5567609 (+) 1140 WP_025340970.1 homoserine O-acetyltransferase -
  C163_RS24470 (C163_24880) metW 5567617..5568237 (+) 621 WP_015272149.1 methionine biosynthesis protein MetW -
  C163_RS24475 (C163_24885) - 5568260..5568694 (+) 435 WP_025340971.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 332 a.a.        Molecular weight: 35932.53 Da        Isoelectric Point: 6.1044

>NTDB_id=115703 C163_RS24445 WP_025340967.1 5563162..5564160(-) (pilT) [Pseudomonas sp. FGI182]
MDVTDLLARAVDAGASDLHLAAGQIPMLRLDGELQRMALPTLGPAALTEGMAPMLDEDQRRQWTRGDELDLALELPVLGR
FRLNLFRQLHGPAATFRLIPGHIATLDELDLGDVFQAVAQCSDGLILVGGPTGSGKSSTLATLLDQLNRDRALHIITLED
PVEVIHSSQRSLVNQREVGRHCGGFAQGLRSALRQDPDVIMIGELRDLETIRLALRAAETGHLVLATVHTRSAVSSIDRL
VEVFAAEEKPLVRAMLAESLRLVVAQVLVRRTGGGRVAAREVLVVTPAVRNLVREGRMAQLCSVMQAGGADGMRTMEGAM
RGLRERGLIEDL

Nucleotide


Download         Length: 999 bp        

>NTDB_id=115703 C163_RS24445 WP_025340967.1 5563162..5564160(-) (pilT) [Pseudomonas sp. FGI182]
ATGGATGTGACCGACCTGTTGGCCCGGGCCGTGGATGCGGGGGCTTCCGACCTGCACCTGGCGGCGGGCCAGATACCGAT
GCTGCGCCTGGATGGCGAGCTGCAGCGCATGGCCCTGCCGACCTTGGGCCCGGCTGCCTTGACCGAGGGCATGGCACCCA
TGCTGGATGAAGACCAGCGTCGACAGTGGACCCGGGGTGATGAGCTGGACCTGGCGCTGGAATTGCCGGTGCTGGGGCGC
TTTCGGCTGAACCTGTTTCGCCAGTTGCATGGCCCTGCGGCCACCTTTCGCCTGATCCCGGGGCATATCGCCACGCTCGA
TGAACTCGACCTGGGGGATGTGTTTCAAGCTGTTGCGCAATGCAGCGATGGCTTGATCCTCGTGGGTGGGCCGACCGGCA
GCGGCAAGTCCAGCACCTTGGCGACACTGCTTGACCAACTGAACCGCGATCGGGCTCTGCATATCATCACCCTCGAAGAC
CCTGTCGAAGTTATCCACAGTAGCCAGCGCAGCCTGGTCAACCAGCGTGAGGTCGGCCGCCATTGCGGTGGGTTCGCCCA
GGGGCTGCGCAGTGCCCTGCGTCAGGACCCGGATGTGATCATGATCGGTGAGCTGCGCGACCTGGAAACCATTCGGCTGG
CCTTGCGCGCGGCCGAGACTGGGCACCTGGTGCTGGCGACCGTGCATACGCGTTCGGCGGTGAGCAGTATTGACCGGTTG
GTGGAGGTGTTCGCTGCCGAAGAGAAGCCGCTGGTGCGGGCGATGCTGGCCGAGTCGTTGCGCCTGGTGGTGGCGCAGGT
GCTGGTCAGGCGTACGGGCGGTGGGCGGGTTGCTGCGCGGGAAGTGCTGGTGGTGACGCCAGCGGTGCGTAACCTGGTTC
GGGAGGGGCGGATGGCGCAGTTGTGTTCGGTGATGCAGGCGGGTGGTGCGGACGGGATGCGGACGATGGAGGGGGCGATG
CGGGGGCTGAGGGAGCGAGGGCTGATCGAAGACTTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

52.28

99.096

0.518
  pilT Acinetobacter baumannii D1279779

51.368

99.096

0.509
  pilT Acinetobacter nosocomialis M2

51.368

99.096

0.509
  pilT Acinetobacter baumannii strain A118

51.368

99.096

0.509
  pilT Pseudomonas stutzeri DSM 10701

51.064

99.096

0.506
  pilT Acinetobacter baylyi ADP1

50.909

99.398

0.506
  pilT Vibrio cholerae strain A1552

49.394

99.398

0.491
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.394

99.398

0.491
  pilT Legionella pneumophila strain Lp02

48.936

99.096

0.485
  pilT Legionella pneumophila strain ERS1305867

48.936

99.096

0.485
  pilT Neisseria meningitidis 8013

48.182

99.398

0.479
  pilT Neisseria gonorrhoeae MS11

47.879

99.398

0.476
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.061

99.398

0.458
  pilU Pseudomonas stutzeri DSM 10701

37.576

99.398

0.373
  pilF Thermus thermophilus HB27

31.538

100

0.37

Multiple sequence alignment