Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilE   Type   Machinery gene
Locus tag   LG374_RS08160 Genome accession   NZ_OU745373
Coordinates   1669321..1669761 (+) Length   146 a.a.
NCBI ID   WP_304518576.1    Uniprot ID   -
Organism   Cardiobacterium sp. Marseille-Q4385     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 1664321..1674761
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LG374_RS08120 rpsO 1665794..1666060 (-) 267 WP_006985646.1 30S ribosomal protein S15 -
  LG374_RS08125 - 1666294..1667727 (+) 1434 WP_225757224.1 DASS family sodium-coupled anion symporter -
  LG374_RS08130 - 1667724..1668095 (+) 372 WP_225757225.1 hypothetical protein -
  LG374_RS08140 pilE 1668116..1668559 (+) 444 WP_304518575.1 pilin Machinery gene
  LG374_RS08150 - 1668604..1669110 (+) 507 WP_370639309.1 pilin -
  LG374_RS08160 pilE 1669321..1669761 (+) 441 WP_304518576.1 pilin Machinery gene
  LG374_RS08165 - 1669948..1673763 (+) 3816 WP_225757226.1 fused MFS/spermidine synthase -
  LG374_RS08170 tfpZ 1673840..1674577 (+) 738 WP_225757227.1 TfpX/TfpZ family type IV pilin accessory protein -

Sequence


Protein


Download         Length: 146 a.a.        Molecular weight: 15330.40 Da        Isoelectric Point: 8.9802

>NTDB_id=1151540 LG374_RS08160 WP_304518576.1 1669321..1669761(+) (pilE) [Cardiobacterium sp. Marseille-Q4385]
MKNYQKGFTLIELMIVIAIIGILAAIALPSYQDYLARSQMTEGLNLAGGQKSSVTEYRSNKGAWPSSNTEAGIPAPSDIA
GKYVASVTVSASGSNGNIRALMKSSGVSARISGAELILQGTETSGAYAWTCKSSVNDRFLPSSCRD

Nucleotide


Download         Length: 441 bp        

>NTDB_id=1151540 LG374_RS08160 WP_304518576.1 1669321..1669761(+) (pilE) [Cardiobacterium sp. Marseille-Q4385]
ATGAAAAACTATCAAAAAGGCTTTACGCTCATTGAACTAATGATCGTTATCGCTATTATTGGCATATTGGCGGCTATTGC
ACTGCCTTCTTATCAAGACTATCTGGCACGTTCACAAATGACGGAAGGATTGAATTTGGCAGGAGGACAAAAGAGTTCGG
TAACTGAATACCGCTCTAATAAAGGTGCGTGGCCTAGCTCCAATACTGAAGCAGGTATTCCGGCTCCCAGCGATATTGCA
GGAAAATATGTTGCGAGTGTAACTGTATCAGCCAGTGGCAGCAATGGTAATATTAGGGCGCTGATGAAAAGCAGTGGCGT
TTCAGCCCGCATTAGCGGTGCTGAACTCATTTTGCAAGGCACAGAAACCAGCGGTGCCTACGCTTGGACCTGTAAATCCA
GTGTGAACGATCGCTTCCTGCCTTCCAGCTGCCGCGACTAG


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae MS11

50

100

0.562

  pilE Neisseria gonorrhoeae strain FA1090

46.626

100

0.521

  comP Acinetobacter baylyi ADP1

43.226

100

0.459

  pilA2 Legionella pneumophila str. Paris

41.958

97.945

0.411

  pilA2 Legionella pneumophila strain ERS1305867

41.958

97.945

0.411

  pilA Acinetobacter baumannii strain A118

41.259

97.945

0.404

  pilA Haemophilus influenzae 86-028NP

40

99.315

0.397

  pilA/pilA1 Eikenella corrodens VA1

37.179

100

0.397

  pilA Ralstonia pseudosolanacearum GMI1000

33.728

100

0.39

  pilA Vibrio cholerae strain A1552

36.364

100

0.384

  pilA Vibrio cholerae O1 biovar El Tor strain E7946

36.364

100

0.384

  pilA Vibrio cholerae C6706

36.364

100

0.384

  pilA Vibrio campbellii strain DS40M4

36

100

0.37

  pilA/pilAI Pseudomonas stutzeri DSM 10701

37.589

96.575

0.363

  pilA/pilAII Pseudomonas stutzeri DSM 10701

34.868

100

0.363


Multiple sequence alignment