Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DBR02_RS06055 Genome accession   NZ_LT991957
Coordinates   1190632..1191612 (-) Length   326 a.a.
NCBI ID   WP_107533723.1    Uniprot ID   -
Organism   Enterobacter cloacae complex sp. isolate C45     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1185632..1196612
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DBR02_RS06025 - 1186521..1187018 (+) 498 WP_107533715.1 SprT family zinc-dependent metalloprotease -
  DBR02_RS06030 endA 1187113..1187820 (+) 708 WP_063867834.1 deoxyribonuclease I -
  DBR02_RS06035 rsmE 1187872..1188603 (+) 732 WP_107533717.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  DBR02_RS06040 gshB 1188623..1189570 (+) 948 WP_107533719.1 glutathione synthase -
  DBR02_RS06045 - 1189645..1190205 (+) 561 WP_022649353.1 YqgE/AlgH family protein -
  DBR02_RS06050 ruvX 1190205..1190621 (+) 417 WP_107533721.1 Holliday junction resolvase RuvX -
  DBR02_RS06055 pilT 1190632..1191612 (-) 981 WP_107533723.1 type IV pilus twitching motility protein PilT Machinery gene
  DBR02_RS06060 - 1191630..1192331 (+) 702 WP_063867830.1 YggS family pyridoxal phosphate-dependent enzyme -
  DBR02_RS06065 - 1192353..1192919 (+) 567 WP_107533725.1 YggT family protein -
  DBR02_RS06070 yggU 1192916..1193212 (+) 297 WP_003860023.1 DUF167 family protein YggU -
  DBR02_RS06075 - 1193216..1193809 (+) 594 WP_107533727.1 XTP/dITP diphosphatase -
  DBR02_RS06080 hemW 1193802..1194944 (+) 1143 WP_063867827.1 radical SAM family heme chaperone HemW -
  DBR02_RS06085 - 1195008..1195346 (+) 339 WP_063451228.1 endonuclease domain-containing protein -
  DBR02_RS06090 - 1195430..1196146 (-) 717 WP_107533729.1 DUF2884 domain-containing protein -
  DBR02_RS06095 - 1196204..1196530 (-) 327 WP_006178455.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35632.66 Da        Isoelectric Point: 6.4998

>NTDB_id=1149131 DBR02_RS06055 WP_107533723.1 1190632..1191612(-) (pilT) [Enterobacter cloacae complex sp. isolate C45]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRLGRLEPAPFPPPDVEGLIKAWLNDEQQGTWWANGQVDFAVTLAGGQRLRG
SAFKHMHGASIALRLLPMTCPQLSALGVPRAIPALLTHDNGLILVTGATGSGKSTTLAAMVDFLNHQTDGHILTLEDPVE
FMYQSERCLIQQREIGQHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLRQDLQGGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=1149131 DBR02_RS06055 WP_107533723.1 1190632..1191612(-) (pilT) [Enterobacter cloacae complex sp. isolate C45]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGGTTAGGCCGGCTTGAACCGGCGCCGTTTCCGCCCCCGGATGTGGAGGGATTAATAAAAGCGTGGCTCAACG
ACGAACAGCAGGGCACATGGTGGGCAAACGGGCAGGTGGATTTTGCCGTTACCCTCGCAGGCGGTCAGCGGCTGCGCGGC
AGTGCGTTTAAGCATATGCACGGCGCTTCGATCGCGCTGCGGCTGTTGCCGATGACGTGCCCGCAGCTCTCTGCATTGGG
CGTGCCGCGAGCGATCCCGGCGCTGTTGACCCATGACAATGGCCTGATTCTGGTCACCGGCGCCACCGGCAGCGGAAAAT
CGACCACCCTGGCCGCGATGGTCGATTTCCTCAATCACCAGACGGACGGGCATATTCTGACCCTGGAAGATCCGGTGGAG
TTTATGTACCAGAGCGAACGTTGCCTGATCCAGCAGCGCGAGATAGGCCAGCACAGCCCGTCCTTTGCCGAGGCCCTGCG
CAGCGCCCTACGTGAAGATCCGGATGTGATTCTGCTTGGCGAGCTGCGCGACAGCGAGACGATACGCCTGGCGCTGACGG
CGGCTGAGACCGGTCATCTGGTTTTAGCCACGCTGCACACGCGCGGGGCGGCGCAGGCCATTGAGCGCCTGGTCGATACC
TTCCCGGCACAGGAAAAAGATCCGGTGCGCAATCAGCTGGCGGGAAGCCTGCGCGCGGTGCTGGCGCAGAAGCTGCGTCA
GGATCTCCAGGGCGGGCGCGTGGCGCTGTACGAATTACTGGTGAACACGTCTGCGGCGGCGAACCTGATCCGCGAAGGCA
AAACCTGGCAACTTCCGGGCATTATCCAAACGGGGCAGCAGGCGGGGATGCAGAACTTTGAGCAGAGTCTGGCAGAGCGG
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(75-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Legionella pneumophila strain Lp02

48.758

98.773

0.482
  pilT Legionella pneumophila strain ERS1305867

48.758

98.773

0.482
  pilT Pseudomonas stutzeri DSM 10701

48.012

100

0.482
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Acinetobacter baumannii strain A118

47.401

100

0.475
  pilT Acinetobacter baumannii D1279779

47.401

100

0.475
  pilT Acinetobacter baylyi ADP1

47.401

100

0.475
  pilT Pseudomonas aeruginosa PAK

47.401

100

0.475
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Acinetobacter nosocomialis M2

47.812

98.16

0.469
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.724

99.08

0.423
  pilU Pseudomonas stutzeri DSM 10701

38.485

100

0.39
  pilU Vibrio cholerae strain A1552

37.578

98.773

0.371

Multiple sequence alignment