Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DBR08_RS22830 Genome accession   NZ_LT991954
Coordinates   4708895..4709875 (-) Length   326 a.a.
NCBI ID   WP_017382924.1    Uniprot ID   -
Organism   Enterobacter hormaechei subsp. steigerwaltii isolate C309     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4703895..4714875
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DBR08_RS22800 - 4704772..4705269 (+) 498 WP_015571833.1 SprT family zinc-dependent metalloprotease -
  DBR08_RS22805 endA 4705364..4706071 (+) 708 WP_003860034.1 deoxyribonuclease I -
  DBR08_RS22810 rsmE 4706123..4706854 (+) 732 WP_017382923.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  DBR08_RS22815 gshB 4706886..4707833 (+) 948 WP_015571835.1 glutathione synthase -
  DBR08_RS22820 - 4707908..4708468 (+) 561 WP_006811924.1 YqgE/AlgH family protein -
  DBR08_RS22825 ruvX 4708468..4708884 (+) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  DBR08_RS22830 pilT 4708895..4709875 (-) 981 WP_017382924.1 type IV pilus twitching motility protein PilT Machinery gene
  DBR08_RS22835 - 4709893..4710594 (+) 702 WP_017382925.1 YggS family pyridoxal phosphate-dependent enzyme -
  DBR08_RS22840 - 4710616..4711182 (+) 567 WP_015571838.1 YggT family protein -
  DBR08_RS22845 yggU 4711179..4711475 (+) 297 WP_003860023.1 DUF167 family protein YggU -
  DBR08_RS22850 - 4711479..4712072 (+) 594 WP_015571839.1 XTP/dITP diphosphatase -
  DBR08_RS22855 hemW 4712065..4713213 (+) 1149 WP_017382926.1 radical SAM family heme chaperone HemW -
  DBR08_RS22860 - 4713270..4713608 (+) 339 WP_017382927.1 endonuclease domain-containing protein -
  DBR08_RS22865 - 4713692..4714408 (-) 717 WP_017382928.1 DUF2884 domain-containing protein -
  DBR08_RS22870 - 4714465..4714791 (-) 327 WP_003862421.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35665.59 Da        Isoelectric Point: 6.0405

>NTDB_id=1149122 DBR08_RS22830 WP_017382924.1 4708895..4709875(-) (pilT) [Enterobacter hormaechei subsp. steigerwaltii isolate C309]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVEALLKAWLNDEQQGAWWANGQVDFAVTLADGQRLRG
SAFKHMHGVSIALRLLPLTCPQLSALGVPRAIPELLSNDNGLILVTGATGSGKSTTLAAMVDFLNHQTDGHILTLEDPVE
FMYQSERCLIQQREIGQHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLRQDVQGGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=1149122 DBR08_RS22830 WP_017382924.1 4708895..4709875(-) (pilT) [Enterobacter hormaechei subsp. steigerwaltii isolate C309]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGGTCAGGCCGTCTTGAACCTGCGCCGTTTCCGCCCCCGGATGTGGAGGCATTATTAAAAGCGTGGCTCAACG
ATGAACAGCAGGGCGCATGGTGGGCAAACGGGCAGGTGGATTTTGCCGTTACCCTCGCAGACGGTCAGCGGCTGCGCGGC
AGTGCGTTTAAGCATATGCACGGCGTTTCGATTGCGCTGCGGCTGTTGCCGCTGACGTGCCCGCAGCTCTCTGCGTTAGG
TGTGCCGCGAGCGATCCCGGAACTGCTGTCTAATGACAATGGCCTGATTCTGGTCACCGGCGCCACCGGCAGCGGAAAAT
CGACCACCCTGGCCGCGATGGTCGATTTCCTCAATCATCAGACAGACGGGCATATTCTGACCCTGGAAGATCCGGTGGAG
TTTATGTACCAGAGCGAACGTTGCCTGATCCAGCAGCGGGAGATAGGCCAGCACAGCCCGTCCTTTGCCGAGGCGCTACG
CAGCGCGCTGCGTGAAGATCCGGATGTGATTCTGCTTGGTGAGCTGCGCGACAGCGAGACGATACGCCTGGCGCTGACGG
CGGCGGAAACCGGCCATCTGGTGTTAGCCACGCTGCATACGCGCGGCGCGGCGCAGGCGATTGAGCGCCTGGTCGACACC
TTTCCGGCGCAGGAGAAAGATCCGGTGCGTAATCAGCTGGCGGGAAGCCTGCGTGCGGTCCTGGCGCAGAAGCTGCGTCA
GGATGTCCAGGGCGGGCGCGTGGCGCTGTATGAGCTGCTGGTGAATACGTCGGCGGCGGCCAACCTGATCCGCGAAGGCA
AAACCTGGCAACTGCCGGGCATTATTCAAACGGGGCAGCAGGCGGGAATGCAGAACTTTGAGCAGAGTCTGGCGGAGCGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(72-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Legionella pneumophila strain Lp02

48.758

98.773

0.482
  pilT Legionella pneumophila strain ERS1305867

48.758

98.773

0.482
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Pseudomonas stutzeri DSM 10701

47.706

100

0.479
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Acinetobacter baumannii strain A118

47.095

100

0.472
  pilT Acinetobacter baumannii D1279779

47.095

100

0.472
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Pseudomonas aeruginosa PAK

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

47.5

98.16

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.653

99.08

0.433
  pilU Pseudomonas stutzeri DSM 10701

37.879

100

0.383
  pilU Vibrio cholerae strain A1552

38.199

98.773

0.377

Multiple sequence alignment