Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DXE35_RS01910 Genome accession   NZ_LT606949
Coordinates   327281..328282 (-) Length   333 a.a.
NCBI ID   WP_114689377.1    Uniprot ID   -
Organism   Polynucleobacter necessarius isolate PPGSP7     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 322281..333282
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DXE35_RS01870 - 322477..323319 (-) 843 WP_114689369.1 hypothetical protein -
  DXE35_RS01875 - 323220..323942 (-) 723 WP_162784938.1 PilC/PilY family type IV pilus protein -
  DXE35_RS09200 - 323917..324288 (-) 372 WP_162784939.1 hypothetical protein -
  DXE35_RS01880 - 324293..325015 (-) 723 WP_114689371.1 hypothetical protein -
  DXE35_RS09205 - 325025..325204 (-) 180 WP_162784940.1 hypothetical protein -
  DXE35_RS01885 - 325238..325573 (-) 336 WP_114689372.1 hypothetical protein -
  DXE35_RS01890 - 325703..326041 (-) 339 WP_114689373.1 hypothetical protein -
  DXE35_RS01895 - 326056..326211 (-) 156 WP_114689374.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  DXE35_RS11295 - 326452..326715 (-) 264 WP_114689375.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  DXE35_RS01905 - 326949..327284 (-) 336 WP_114689376.1 Tfp pilus assembly protein FimT/FimU -
  DXE35_RS01910 pilT 327281..328282 (-) 1002 WP_114689377.1 type IV pilus twitching motility protein PilT Machinery gene
  DXE35_RS01915 - 328304..328528 (-) 225 WP_114689378.1 prepilin peptidase -
  DXE35_RS01920 - 328532..329578 (-) 1047 WP_114689379.1 NAD(P)/FAD-dependent oxidoreductase -
  DXE35_RS01925 fdxA 329598..329921 (-) 324 WP_114689380.1 ferredoxin FdxA -
  DXE35_RS01930 - 330040..331053 (+) 1014 WP_114689381.1 2-hydroxyacid dehydrogenase -
  DXE35_RS01935 - 331127..331420 (-) 294 WP_072583151.1 hypothetical protein -
  DXE35_RS10060 - 331433..332146 (-) 714 WP_231969927.1 molybdopterin molybdotransferase MoeA -
  DXE35_RS10885 - 332107..332358 (-) 252 WP_331851950.1 hypothetical protein -
  DXE35_RS10890 - 332394..332753 (-) 360 WP_269459847.1 hypothetical protein -

Sequence

Protein


Download         Length: 333 a.a.        Molecular weight: 37314.83 Da        Isoelectric Point: 5.6778

>NTDB_id=1145197 DXE35_RS01910 WP_114689377.1 327281..328282(-) (pilT) [Polynucleobacter necessarius isolate PPGSP7]
MEIIDKALHYVTDYKISDLHLHVNEPVAIRVDGDIHTFAADIVTQVDFDSFIKNWLTQEQRLKFMECFDADLAVESGEYR
FRVNLFKTARGLAAVMRKIETQIPSFDSLGLPAVARDVIELENGLILVTCPTGSGKSTTLAAMIDRINRQRPGHIMTIED
PIEFVHRSQKSVISQREVARDTLSFSSALRASLREDPDVILVGELRDLETIRLALTAAETGHLVFETLNTGGDPNTINRI
IDVFPFQQQDQVRAQLSQSLRLVMTQRLLSKKGGVGRIGAFEVMTCNPAVRNLIRENKVFQIPSVMQMARGEGRITMEAS
LQQLEQAGQIDPL

Nucleotide


Download         Length: 1002 bp        

>NTDB_id=1145197 DXE35_RS01910 WP_114689377.1 327281..328282(-) (pilT) [Polynucleobacter necessarius isolate PPGSP7]
ATGGAAATCATCGATAAAGCCCTGCACTACGTTACAGATTACAAGATTTCGGACCTGCATTTGCACGTCAATGAGCCTGT
GGCGATACGGGTTGATGGTGATATTCATACGTTCGCGGCTGACATCGTTACCCAAGTCGATTTTGATTCATTCATTAAGA
ATTGGCTGACACAAGAGCAGCGTCTGAAGTTTATGGAATGCTTTGATGCTGACTTGGCGGTGGAGTCGGGTGAGTATCGC
TTCCGTGTGAACTTATTTAAAACGGCCCGCGGTTTGGCGGCGGTGATGCGAAAGATTGAAACGCAAATTCCGAGCTTTGA
TTCTTTGGGATTACCGGCTGTTGCTCGCGATGTGATTGAGCTTGAAAATGGTTTGATATTAGTTACATGCCCGACAGGTT
CAGGCAAGTCAACGACGCTGGCGGCAATGATTGATCGTATTAATCGGCAGCGTCCTGGTCACATTATGACGATCGAGGAC
CCAATTGAGTTCGTACATCGCAGTCAAAAGAGTGTAATTTCACAAAGAGAGGTTGCACGCGATACCCTCTCTTTTTCATC
TGCGCTAAGAGCTTCCTTACGGGAAGATCCCGATGTCATTCTGGTGGGTGAGTTACGCGATCTAGAAACTATTCGGCTTG
CCCTAACCGCTGCTGAAACTGGACACTTAGTATTCGAAACCCTGAATACCGGTGGCGATCCAAACACGATTAACCGAATC
ATCGACGTTTTTCCATTTCAACAACAAGACCAGGTGAGGGCGCAGTTATCACAATCACTTCGTCTGGTGATGACCCAGCG
CTTGCTTAGCAAAAAAGGTGGGGTAGGTCGTATCGGCGCATTCGAAGTGATGACCTGCAATCCAGCGGTGCGCAATCTGA
TACGTGAGAACAAAGTATTTCAAATACCAAGCGTGATGCAAATGGCTCGCGGCGAGGGAAGGATCACCATGGAAGCGTCA
CTGCAGCAATTAGAGCAAGCGGGGCAGATTGACCCATTATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

50.76

98.799

0.502
  pilT Neisseria meningitidis 8013

49.55

100

0.496
  pilT Neisseria gonorrhoeae MS11

49.55

100

0.496
  pilT Vibrio cholerae strain A1552

50.152

98.799

0.495
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.152

98.799

0.495
  pilT Acinetobacter baumannii strain A118

50

99.099

0.495
  pilT Acinetobacter nosocomialis M2

50

99.099

0.495
  pilT Acinetobacter baumannii D1279779

50

99.099

0.495
  pilT Legionella pneumophila strain Lp02

51.242

96.697

0.495
  pilT Legionella pneumophila strain ERS1305867

51.242

96.697

0.495
  pilT Pseudomonas stutzeri DSM 10701

49.695

98.498

0.489
  pilT Pseudomonas aeruginosa PAK

49.39

98.498

0.486
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.428

95.495

0.462
  pilU Vibrio cholerae strain A1552

38.629

96.396

0.372
  pilU Acinetobacter baylyi ADP1

37.618

95.796

0.36

Multiple sequence alignment