Detailed information NTG1142198

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: DQN30_RS04325
• Genome accession: NZ_LS483458
• Coordinates: 851074..851514 (-)
• Length: 146 a.a.
• NCBI ID: WP_111696427.1
• Uniprot ID: -
• Organism: Haemophilus haemolyticus strain NCTC10839
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 846074..856514

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DQN30_RS04305 (NCTC10839_00850)	rho	846461..847723 (+)	1263	WP_005629550.1	transcription termination factor Rho	-
DQN30_RS04310 (NCTC10839_00851)	pilD	847777..848469 (-)	693	WP_111696424.1	A24 family peptidase	Machinery gene
DQN30_RS04315 (NCTC10839_00852)	pilC	848466..849686 (-)	1221	WP_111696425.1	type II secretion system F family protein	Machinery gene
DQN30_RS04320 (NCTC10839_00853)	pilB	849683..851077 (-)	1395	WP_111696426.1	GspE/PulE family protein	Machinery gene
DQN30_RS04325 (NCTC10839_00854)	pilA	851074..851514 (-)	441	WP_111696427.1	prepilin peptidase-dependent pilin	Machinery gene
DQN30_RS04330 (NCTC10839_00855)	ampD	851629..852183 (+)	555	WP_111696428.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
DQN30_RS04335 (NCTC10839_00856)	rppH	852709..853299 (+)	591	WP_111696429.1	RNA pyrophosphohydrolase	-
DQN30_RS04340 (NCTC10839_00857)	-	853299..854096 (+)	798	WP_111696430.1	sulfite exporter TauE/SafE family protein	-
DQN30_RS04345 (NCTC10839_00858)	lgt	854105..854911 (+)	807	WP_111696431.1	prolipoprotein diacylglyceryl transferase	-
DQN30_RS04350 (NCTC10839_00859)	-	854922..855773 (+)	852	WP_111696432.1	thymidylate synthase	-
DQN30_RS04355 (NCTC10839_00860)	tadA	855773..856288 (+)	516	WP_111696433.1	tRNA adenosine(34) deaminase TadA	-

Sequence

Protein

• Length: 146 a.a.
• Molecular weight: 15107.29 Da
• Isoelectric Point: 8.0922

>NTDB_id=1142198 DQN30_RS04325 WP_111696427.1 851074..851514(-) (pilA) [Haemophilus haemolyticus strain NCTC10839]
MKLTTQTTLKKGFTLIELMIVIAIIAILATIAIPSYQNYTKKAAVSELLQASAPYKSDVELCVYSTGKPSSCSGGSNGIA
ADITTAKGYVKSVTTSNGAITVEGNGTLDGMSYTLTAEGNSANGVTWKTTCGTTNVDIFPAGFCSK

Nucleotide

• Length: 441 bp

>NTDB_id=1142198 DQN30_RS04325 WP_111696427.1 851074..851514(-) (pilA) [Haemophilus haemolyticus strain NCTC10839]
ATGAAACTAACTACACAAACTACCTTGAAGAAAGGGTTTACCTTAATTGAATTGATGATAGTCATCGCCATCATCGCGAT
TTTAGCCACTATCGCTATTCCTTCTTATCAAAATTATACCAAAAAAGCCGCGGTATCCGAATTACTGCAAGCTTCTGCAC
CTTATAAGTCAGATGTGGAATTATGCGTTTATAGCACTGGCAAACCTTCCAGTTGCTCGGGAGGAAGCAATGGAATTGCG
GCTGATATTACAACAGCAAAAGGCTATGTAAAATCAGTGACAACAAGCAACGGTGCAATAACAGTAGAGGGCAATGGTAC
ATTAGACGGAATGAGTTACACACTTACTGCTGAAGGCAATAGTGCAAACGGCGTTACATGGAAAACTACCTGTGGCACAA
CGAATGTCGATATTTTCCCTGCTGGATTCTGTTCAAAATGA

Domains

Predicted by InterproScan.

• Pilin (bacterial filament) (Pilin) (42-137)

• Prokaryotic N-terminal methylation motif (N_methyl) (10-32)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Haemophilus influenzae 86-028NP	81.25	98.63	0.801
pilA	Haemophilus influenzae Rd KW20	77.778	98.63	0.767
pilA	Glaesserella parasuis strain SC1401	55.333	100	0.568
pilA2	Legionella pneumophila str. Paris	39.86	97.945	0.39
pilA2	Legionella pneumophila strain ERS1305867	39.86	97.945	0.39
comP	Acinetobacter baylyi ADP1	42.748	89.726	0.384
pilA	Vibrio cholerae C6706	37.241	99.315	0.37
pilA	Vibrio cholerae strain A1552	37.241	99.315	0.37
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	37.241	99.315	0.37
pilA	Vibrio campbellii strain DS40M4	36.552	99.315	0.363
pilA/pilAI	Pseudomonas stutzeri DSM 10701	36.301	100	0.363