Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   DQN30_RS04320 Genome accession   NZ_LS483458
Coordinates   849683..851077 (-) Length   464 a.a.
NCBI ID   WP_111696426.1    Uniprot ID   A0A2X4RKD4
Organism   Haemophilus haemolyticus strain NCTC10839     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 844683..856077
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQN30_RS04290 (NCTC10839_00847) - 845213..845419 (-) 207 WP_005631184.1 heavy-metal-associated domain-containing protein -
  DQN30_RS04295 (NCTC10839_00848) - 845496..845882 (+) 387 WP_111696423.1 Cu(I)-responsive transcriptional regulator -
  DQN30_RS04300 (NCTC10839_00849) metJ 845896..846213 (-) 318 WP_005634287.1 met regulon transcriptional regulator MetJ -
  DQN30_RS04305 (NCTC10839_00850) rho 846461..847723 (+) 1263 WP_005629550.1 transcription termination factor Rho -
  DQN30_RS04310 (NCTC10839_00851) pilD 847777..848469 (-) 693 WP_111696424.1 A24 family peptidase Machinery gene
  DQN30_RS04315 (NCTC10839_00852) pilC 848466..849686 (-) 1221 WP_111696425.1 type II secretion system F family protein Machinery gene
  DQN30_RS04320 (NCTC10839_00853) pilB 849683..851077 (-) 1395 WP_111696426.1 GspE/PulE family protein Machinery gene
  DQN30_RS04325 (NCTC10839_00854) pilA 851074..851514 (-) 441 WP_111696427.1 prepilin peptidase-dependent pilin Machinery gene
  DQN30_RS04330 (NCTC10839_00855) ampD 851629..852183 (+) 555 WP_111696428.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  DQN30_RS04335 (NCTC10839_00856) rppH 852709..853299 (+) 591 WP_111696429.1 RNA pyrophosphohydrolase -
  DQN30_RS04340 (NCTC10839_00857) - 853299..854096 (+) 798 WP_111696430.1 sulfite exporter TauE/SafE family protein -
  DQN30_RS04345 (NCTC10839_00858) lgt 854105..854911 (+) 807 WP_111696431.1 prolipoprotein diacylglyceryl transferase -
  DQN30_RS04350 (NCTC10839_00859) - 854922..855773 (+) 852 WP_111696432.1 thymidylate synthase -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 52581.90 Da        Isoelectric Point: 5.7840

>NTDB_id=1142197 DQN30_RS04320 WP_111696426.1 849683..851077(-) (pilB) [Haemophilus haemolyticus strain NCTC10839]
MIDLTSAKPRVKAQNGEIFTISPDLWGRNQQQQSLLLRYFALPLKEENNRLWLGVDSLSNLSACETIAFITGKLVEPVLL
ESTQLKELLQQLAPNQLQVEEQVKYYQHQEQSHLEQQDDEPVIRLLNQIFESALQNNASDIHLETLADHFQVRFRIDGVL
QAQPPLSKNLANRIISRLKLLAKLDISETRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKVVLRAQQNKSVELSFSELG
MTESQQKEFQRALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELDGIIQSQINPQIGLDFSRLLR
AFLRQDPDIIMLGEIRDEESAMIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQHEIENSLLLVIAQRLVRKICPKC
GGDLINSCDCHQGYQGRIGVYQFLHWQQNGYQTDFKNLHTSGLEKVSQGITDEKEIERVLGKNS

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=1142197 DQN30_RS04320 WP_111696426.1 849683..851077(-) (pilB) [Haemophilus haemolyticus strain NCTC10839]
ATGATCGACTTAACTAGTGCCAAACCTCGTGTAAAAGCACAAAATGGCGAGATATTTACGATCTCGCCTGATTTATGGGG
GCGTAATCAGCAACAACAGTCTCTGCTCTTACGTTATTTTGCTTTACCACTCAAAGAAGAAAATAATCGTCTTTGGTTAG
GTGTTGATTCCCTTTCTAATCTTTCTGCCTGTGAAACTATTGCGTTTATAACCGGTAAACTTGTTGAGCCTGTTTTATTA
GAAAGCACTCAACTTAAAGAACTATTACAACAACTTGCCCCGAATCAACTTCAAGTGGAAGAGCAAGTTAAATATTACCA
ACATCAAGAGCAGTCTCATCTTGAACAACAAGATGATGAACCCGTCATTCGCTTGCTTAATCAAATTTTTGAATCCGCTT
TACAAAACAATGCCTCAGATATTCATTTGGAGACCTTAGCGGATCATTTCCAAGTGCGGTTTAGAATTGACGGTGTTTTA
CAAGCTCAGCCTCCACTTAGCAAAAATTTAGCGAATCGAATTATTTCTCGCCTAAAACTGCTCGCTAAATTAGATATCAG
TGAAACCCGCCTTCCACAAGATGGACGATTTCAATTTAAAACTACGTTTTCCGATATTCTTGATTTCCGTCTTTCAACGT
TACCAACTCATTGGGGAGAGAAAGTTGTCCTGAGAGCACAGCAAAATAAATCAGTAGAACTCAGCTTTTCTGAACTAGGC
ATGACTGAAAGTCAACAGAAAGAATTTCAACGCGCGCTTAGCCAACCACAAGGATTAATTTTAGTAACCGGCCCAACAGG
AAGTGGGAAAAGTATCTCACTTTACACCGCACTTCAGTGGCTAAACACGCCTGATAAACATATTATGACCGCTGAGGATC
CCATTGAAATTGAGCTTGATGGAATTATTCAAAGCCAAATTAATCCACAAATAGGACTAGATTTTAGCCGTCTATTGCGC
GCTTTTTTACGTCAAGATCCCGACATCATTATGCTAGGTGAAATTCGCGATGAAGAAAGTGCAATGATTGCACTACGTGC
CGCCCAAACGGGGCATTTGGTGCTTTCAACTTTACATACCAATGATGCAATATCTGCCATTTCTCGCTTACAACAACTCG
GTATTCAACAACATGAGATTGAAAACAGTTTGCTGCTCGTCATTGCTCAGCGTCTTGTACGAAAAATCTGTCCAAAGTGC
GGTGGAGATTTAATAAATTCTTGTGATTGCCATCAAGGTTATCAAGGTCGAATCGGCGTGTATCAATTTCTACATTGGCA
GCAGAATGGCTATCAAACGGATTTTAAAAATTTACATACGAGTGGTTTAGAAAAAGTTAGCCAAGGCATAACAGATGAGA
AAGAAATTGAACGTGTGTTAGGTAAAAACTCATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(31-96)

Type II/IV secretion system protein (T2SSE)

(128-395)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2X4RKD4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

92.527

98.06

0.907
  pilB Haemophilus influenzae Rd KW20

91.189

97.845

0.892
  pilB Glaesserella parasuis strain SC1401

58.114

98.276

0.571
  pilB Vibrio campbellii strain DS40M4

41.309

100

0.435
  pilB Legionella pneumophila strain ERS1305867

40.08

100

0.431
  pilB Vibrio parahaemolyticus RIMD 2210633

41.372

100

0.429
  pilB Vibrio cholerae strain A1552

40.619

100

0.425
  pilB Acinetobacter baumannii D1279779

39.752

100

0.414
  pilB Acinetobacter baylyi ADP1

38.277

100

0.412
  pilF Neisseria gonorrhoeae MS11

37.605

100

0.386
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.313

100

0.377
  pilF Thermus thermophilus HB27

36

100

0.369

Multiple sequence alignment