Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DQN94_RS03825 Genome accession   NZ_LS483455
Coordinates   765229..766209 (+) Length   326 a.a.
NCBI ID   WP_111761345.1    Uniprot ID   -
Organism   Salmonella enterica subsp. enterica serovar Carmel strain NCTC9872     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 760229..771209
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQN94_RS03795 (NCTC9872_00745) - 760807..761814 (+) 1008 WP_000252190.1 DUF1202 domain-containing protein -
  DQN94_RS03800 (NCTC9872_00746) hemW 761905..763041 (-) 1137 WP_023195147.1 radical SAM family heme chaperone HemW -
  DQN94_RS03805 (NCTC9872_00747) - 763034..763627 (-) 594 WP_001174769.1 XTP/dITP diphosphatase -
  DQN94_RS03810 (NCTC9872_00748) yggU 763635..763925 (-) 291 WP_001277205.1 DUF167 family protein YggU -
  DQN94_RS03815 (NCTC9872_00749) - 763922..764488 (-) 567 WP_001094848.1 YggT family protein -
  DQN94_RS03820 (NCTC9872_00750) - 764507..765211 (-) 705 WP_000997812.1 YggS family pyridoxal phosphate-dependent enzyme -
  DQN94_RS03825 (NCTC9872_00751) pilT 765229..766209 (+) 981 WP_111761345.1 type IV pilus twitching motility protein PilT Machinery gene
  DQN94_RS03830 (NCTC9872_00752) - 766339..767040 (+) 702 WP_000098326.1 IclR family transcriptional regulator -
  DQN94_RS03835 (NCTC9872_00753) ruvX 767072..767488 (-) 417 WP_000017096.1 Holliday junction resolvase RuvX -
  DQN94_RS03840 (NCTC9872_00754) - 767488..768051 (-) 564 WP_001053175.1 YqgE/AlgH family protein -
  DQN94_RS03845 (NCTC9872_00755) gshB 768267..769214 (-) 948 WP_023137233.1 glutathione synthase -
  DQN94_RS03850 (NCTC9872_00756) rsmE 769234..769965 (-) 732 WP_001222488.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  DQN94_RS03855 (NCTC9872_00757) endA 770042..770749 (-) 708 WP_111761346.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36118.38 Da        Isoelectric Point: 7.5116

>NTDB_id=1142134 DQN94_RS03825 WP_111761345.1 765229..766209(+) (pilT) [Salmonella enterica subsp. enterica serovar Carmel strain NCTC9872]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMEIAPFTAPDVDRLLLDWLNDAQQYQWRTHGQLDFAVSLAGTRRLRA
SAFTHQQGTSLALRLLPERCPDLAEIQTPPIVPALLASENGLILVTGATGCGKSTTLAAMVGYLNQHADKHILTLEDPIE
YRYTSKRCLIQQREIGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPIRSQLAGSLRAVLSQKLEVDRQDGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMAFAQSAQWR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=1142134 DQN94_RS03825 WP_111761345.1 765229..766209(+) (pilT) [Salmonella enterica subsp. enterica serovar Carmel strain NCTC9872]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGCATGGAGATCGCGCCTTTTACCGCGCCAGATGTCGACAGGCTTCTGCTTGATTGGCTTAATG
ACGCGCAACAATATCAGTGGCGAACGCACGGCCAGCTTGATTTCGCCGTCTCGCTGGCCGGCACGCGGCGCCTGCGCGCC
AGTGCGTTCACACATCAACAGGGAACGTCGCTGGCGCTACGGCTATTGCCCGAGCGTTGTCCTGATCTGGCGGAAATCCA
GACGCCGCCGATAGTACCCGCACTGCTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGCGCTACTGGCTGCGGGAAGT
CCACCACGCTGGCGGCGATGGTGGGATATCTTAATCAACATGCCGATAAACACATCCTGACGCTGGAAGATCCTATTGAG
TACCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAATCGGCCAGCATTGCGCCACTTTTGCCGCCGGGCTGCG
CGCGGCGTTGCGTGAAGATCCCGATGTCATTTTGCTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGCGGTGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGATACGCAGTCAACTGGCGGGTAGTCTGCGCGCCGTACTATCACAAAAACTGGAGGT
CGACAGACAGGATGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGGCATTCGCCCAGAGCGCGCAATGGCGT
CAGGCGCAGGGACGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-240)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.318

100

0.485
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.318

100

0.485
  pilT Neisseria meningitidis 8013

46.951

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.646

100

0.469
  pilT Acinetobacter baumannii strain A118

44.648

100

0.448
  pilT Acinetobacter baumannii D1279779

44.648

100

0.448
  pilT Acinetobacter baylyi ADP1

44.648

100

0.448
  pilT Pseudomonas aeruginosa PAK

44.648

100

0.448
  pilT Pseudomonas stutzeri DSM 10701

44.343

100

0.445
  pilT Acinetobacter nosocomialis M2

44.343

100

0.445
  pilT Legionella pneumophila strain Lp02

44.654

97.546

0.436
  pilT Legionella pneumophila strain ERS1305867

44.654

97.546

0.436
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.089

96.933

0.408
  pilU Vibrio cholerae strain A1552

38.558

97.853

0.377
  pilU Pseudomonas stutzeri DSM 10701

36.036

100

0.368

Multiple sequence alignment