Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   DQN79_RS05315 Genome accession   NZ_LS483435
Coordinates   1055481..1056620 (-) Length   379 a.a.
NCBI ID   WP_003773128.1    Uniprot ID   A0A1F1SN15
Organism   Neisseria elongata subsp. glycolytica strain NCTC11050     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1050481..1061620
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQN79_RS05290 (NCTC11050_01003) - 1051253..1052701 (+) 1449 WP_040666075.1 dihydrolipoyl dehydrogenase -
  DQN79_RS05295 (NCTC11050_01004) - 1053091..1053456 (-) 366 WP_003773119.1 YkvA family protein -
  DQN79_RS05300 (NCTC11050_01005) - 1053577..1054872 (-) 1296 WP_041961308.1 MFS transporter -
  DQN79_RS05305 (NCTC11050_01006) ccoS 1054888..1055082 (-) 195 WP_003773124.1 cbb3-type cytochrome oxidase assembly protein CcoS -
  DQN79_RS05310 (NCTC11050_01007) - 1055085..1055402 (-) 318 WP_003773126.1 HLGFF motif protein -
  DQN79_RS05315 (NCTC11050_01008) pilU 1055481..1056620 (-) 1140 WP_003773128.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DQN79_RS05320 (NCTC11050_01009) - 1056896..1057369 (+) 474 WP_003773130.1 OsmC family protein -
  DQN79_RS05325 (NCTC11050_01010) - 1057528..1058751 (-) 1224 WP_003773133.1 beta-ketoacyl-ACP synthase -
  DQN79_RS05330 (NCTC11050_01011) fabG 1058751..1059479 (-) 729 WP_003773134.1 3-oxoacyl-ACP reductase FabG -
  DQN79_RS05335 (NCTC11050_01012) - 1059757..1060545 (-) 789 WP_144340415.1 hypothetical protein -
  DQN79_RS05340 (NCTC11050_01013) - 1060616..1061119 (-) 504 WP_003773140.1 hypothetical protein -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42328.71 Da        Isoelectric Point: 6.0051

>NTDB_id=1141400 DQN79_RS05315 WP_003773128.1 1055481..1056620(-) (pilU) [Neisseria elongata subsp. glycolytica strain NCTC11050]
MQTQKPTEPLSIKETIFNWLRYMYEQKASDLFITAEFPPAIKLDGKLTPISDRPLTAGTCGAIAQAIMTEKQREEFENTK
ECNFAISLDGVARFRVNAMIQRGAVALVIRIINSVIPKMDDLKLPEVLKKVSMQKRGLVIFVGGTGSGKSTSLAAMIDYR
NENSYGHIITIEDPIEFVHPHKNCIVTQREVGVDTDNWFAALKNTLRQAPDVILIGEIRDQETMEYALAFAETGHLCMAT
LHANSSNQALDRIINFFPEERRTQLLTDLSLNLQAFVSQRLVPKENGKGRVAAVEVLLQSPLISDLILRGEVHGIKDIMK
KSTDIGMQTFDQALYELFESGQISYAEAIKNADSANDLRLAIQLKSRRGQNAGIDFELL

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=1141400 DQN79_RS05315 WP_003773128.1 1055481..1056620(-) (pilU) [Neisseria elongata subsp. glycolytica strain NCTC11050]
ATGCAAACTCAAAAACCAACCGAGCCGCTGAGCATCAAAGAAACCATCTTCAACTGGCTGCGCTACATGTACGAACAAAA
AGCGTCAGACCTCTTCATCACGGCCGAATTTCCGCCTGCCATCAAACTCGACGGCAAGCTGACCCCCATCAGCGACCGCC
CGCTGACGGCAGGTACCTGCGGCGCAATCGCCCAGGCCATCATGACCGAAAAACAGCGCGAAGAATTCGAAAACACCAAA
GAATGCAACTTCGCCATCAGCCTCGACGGCGTGGCGCGTTTCCGTGTCAACGCCATGATTCAGCGCGGCGCGGTGGCACT
CGTCATCCGCATCATCAACAGCGTGATTCCGAAAATGGACGATCTGAAACTGCCCGAAGTGCTGAAAAAAGTCTCCATGC
AAAAACGCGGGTTGGTGATTTTCGTCGGCGGTACAGGCTCGGGCAAATCCACTTCGCTGGCCGCCATGATCGACTACCGC
AACGAAAACAGCTACGGCCACATTATCACCATTGAAGACCCGATCGAATTCGTCCATCCACACAAAAACTGCATCGTTAC
CCAACGCGAAGTCGGCGTGGACACCGACAACTGGTTCGCCGCCCTGAAAAACACCCTGCGCCAAGCACCCGACGTCATCC
TCATTGGCGAGATTCGCGACCAGGAAACCATGGAATACGCCCTGGCCTTCGCCGAAACCGGCCACCTCTGCATGGCCACG
CTGCACGCCAACAGTTCCAACCAGGCACTCGACCGCATCATCAACTTCTTCCCCGAAGAACGCCGCACCCAACTTCTAAC
CGACCTCTCGCTCAACCTGCAAGCCTTTGTTTCGCAACGGCTGGTTCCGAAAGAAAACGGCAAAGGCCGCGTGGCCGCAG
TGGAAGTGCTGTTACAGTCGCCGCTGATTTCCGACCTCATCCTGCGCGGCGAGGTGCACGGCATCAAAGACATCATGAAA
AAATCCACCGACATCGGTATGCAGACCTTCGACCAAGCCTTATACGAACTGTTCGAAAGCGGCCAAATTTCCTACGCCGA
AGCGATTAAAAATGCCGATTCCGCCAACGATTTGCGTTTGGCCATCCAACTGAAGAGCCGGCGCGGACAAAATGCCGGCA
TCGACTTCGAACTGCTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(25-283)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1F1SN15

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.248

91.557

0.57
  pilU Acinetobacter baylyi ADP1

57.759

91.821

0.53
  pilU Vibrio cholerae strain A1552

54.768

96.834

0.53
  pilT Pseudomonas aeruginosa PAK

44.807

88.918

0.398
  pilT Legionella pneumophila strain ERS1305867

45.289

86.807

0.393
  pilT Legionella pneumophila strain Lp02

45.289

86.807

0.393
  pilT Pseudomonas stutzeri DSM 10701

43.917

88.918

0.391
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.62

88.918

0.388
  pilT Acinetobacter nosocomialis M2

43.769

86.807

0.38
  pilT Acinetobacter baumannii D1279779

43.769

86.807

0.38
  pilT Acinetobacter baumannii strain A118

43.769

86.807

0.38
  pilT Acinetobacter baylyi ADP1

41.888

89.446

0.375
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.265

87.599

0.361
  pilT Vibrio cholerae strain A1552

41.265

87.599

0.361

Multiple sequence alignment