Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ENTER_RS18045 Genome accession   NZ_LR881936
Coordinates   3845757..3846737 (-) Length   326 a.a.
NCBI ID   WP_202561114.1    Uniprot ID   -
Organism   Enterobacter cancerogenus strain UPC1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3840757..3851737
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ENTER_RS18015 (ENTER_3658) - 3841614..3842111 (+) 498 WP_202561113.1 SprT family zinc-dependent metalloprotease -
  ENTER_RS18020 (ENTER_3659) endA 3842206..3842913 (+) 708 WP_006178442.1 deoxyribonuclease I -
  ENTER_RS18025 (ENTER_3660) rsmE 3842966..3843697 (+) 732 WP_202562273.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ENTER_RS18030 (ENTER_3661) gshB 3843719..3844666 (+) 948 WP_042322311.1 glutathione synthase -
  ENTER_RS18035 (ENTER_3662) - 3844749..3845309 (+) 561 WP_137272712.1 YqgE/AlgH family protein -
  ENTER_RS18040 (ENTER_3663) ruvX 3845309..3845725 (+) 417 WP_006178446.1 Holliday junction resolvase RuvX -
  ENTER_RS18045 (ENTER_3664) pilT 3845757..3846737 (-) 981 WP_202561114.1 type IV pilus twitching motility protein PilT Machinery gene
  ENTER_RS18050 (ENTER_3665) - 3846755..3847456 (+) 702 WP_006178448.1 YggS family pyridoxal phosphate-dependent enzyme -
  ENTER_RS18055 (ENTER_3666) - 3847478..3848044 (+) 567 WP_058608623.1 YggT family protein -
  ENTER_RS18060 (ENTER_3667) yggU 3848041..3848337 (+) 297 WP_006178450.1 DUF167 family protein YggU -
  ENTER_RS18065 (ENTER_3668) - 3848341..3848934 (+) 594 WP_202561115.1 XTP/dITP diphosphatase -
  ENTER_RS18070 (ENTER_3669) hemW 3848927..3850069 (+) 1143 WP_058608625.1 radical SAM family heme chaperone HemW -
  ENTER_RS18075 (ENTER_3670) - 3850133..3850495 (+) 363 WP_202561116.1 endonuclease domain-containing protein -
  ENTER_RS18080 (ENTER_3671) - 3850618..3851334 (-) 717 WP_006178454.1 DUF2884 domain-containing protein -
  ENTER_RS18085 (ENTER_3672) - 3851392..3851718 (-) 327 WP_006178455.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35738.71 Da        Isoelectric Point: 6.0414

>NTDB_id=1132672 ENTER_RS18045 WP_202561114.1 3845757..3846737(-) (pilT) [Enterobacter cancerogenus strain UPC1]
MDVEEIVALSVKHNVSDLHLCSDAPPRWRRLGRLEPAPFPPPDVNVLLREWLNDEQQGTWWANGQVDFAVTTGENQRLRG
SAFMHIQGASVALRLLPRACPQLSALGVPRAIPELLLNDSGLLLVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
FIYQSERCLIQQREIGQHSPTFAEALRGALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQGIERLVDT
FPAQEKDPVRTQLAGSLRAVLAQKLRPDLQGGRVALYELLVNTPAAANLIREGKTWQLPGIVQTGQQAGMQNFEQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=1132672 ENTER_RS18045 WP_202561114.1 3845757..3846737(-) (pilT) [Enterobacter cancerogenus strain UPC1]
ATGGATGTGGAAGAAATCGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATGCCCCCCCGCG
CTGGCGGCGATTAGGCCGCCTTGAGCCCGCCCCGTTTCCGCCTCCGGACGTGAACGTGCTGCTCAGGGAGTGGCTTAATG
ATGAACAGCAGGGGACGTGGTGGGCAAACGGGCAGGTGGATTTTGCCGTCACCACCGGCGAAAACCAGCGCCTGCGCGGC
AGCGCGTTTATGCATATACAGGGTGCCTCTGTCGCGCTGCGACTGCTGCCGCGTGCCTGCCCGCAACTTTCTGCGCTGGG
CGTGCCGCGGGCGATCCCGGAGCTGTTACTGAATGACAGCGGGCTGCTGCTGGTGACCGGGGCAACCGGCAGCGGAAAAT
CCACCACTCTGGCGGCGATGGTCGATTTTCTCAACCACCACACCGACGGGCATATTCTGACGCTCGAAGATCCGGTGGAA
TTTATCTATCAGAGTGAACGCTGCCTGATCCAGCAGCGCGAGATAGGCCAGCACAGCCCGACGTTTGCCGAGGCGCTGCG
GGGCGCGTTGCGTGAAGATCCGGATGTGATTTTGCTGGGGGAGCTGCGCGACAGCGAAACGATCCGCCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCAACGCTGCATACGCGTGGGGCATCGCAGGGCATTGAACGGCTGGTGGATACC
TTTCCGGCGCAGGAGAAAGACCCGGTGCGCACCCAGCTGGCGGGCAGCCTGCGGGCGGTTCTGGCGCAAAAGCTGCGCCC
GGATTTGCAGGGCGGGCGCGTCGCGCTTTATGAGCTGCTGGTGAATACCCCGGCGGCGGCGAACCTGATCCGTGAAGGCA
AAACCTGGCAGCTTCCCGGGATCGTTCAAACGGGTCAGCAGGCGGGCATGCAGAACTTTGAACAGAGCCTGGCTGAGCGC
CGGGCGCAGGGCAGGCTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Acinetobacter baylyi ADP1

47.095

100

0.472
  pilT Legionella pneumophila strain Lp02

47.516

98.773

0.469
  pilT Legionella pneumophila strain ERS1305867

47.516

98.773

0.469
  pilT Pseudomonas stutzeri DSM 10701

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.789

100

0.469
  pilT Acinetobacter baumannii strain A118

46.789

100

0.469
  pilT Neisseria meningitidis 8013

46.341

100

0.466
  pilT Acinetobacter nosocomialis M2

47.188

98.16

0.463
  pilT Neisseria gonorrhoeae MS11

46.037

100

0.463
  pilT Pseudomonas aeruginosa PAK

46.177

100

0.463
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.236

98.773

0.417
  pilU Pseudomonas stutzeri DSM 10701

38.788

100

0.393
  pilU Vibrio cholerae strain A1552

37.578

98.773

0.371

Multiple sequence alignment