Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   H1D11_RS06785 Genome accession   NZ_LR778147
Coordinates   1470053..1471033 (+) Length   326 a.a.
NCBI ID   WP_115423636.1    Uniprot ID   -
Organism   Escherichia coli isolate SC418     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1465053..1476033
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H1D11_RS06755 - 1465427..1466413 (-) 987 WP_023141969.1 TRAP transporter substrate-binding protein -
  H1D11_RS06760 hemW 1466730..1467866 (-) 1137 WP_115423637.1 radical SAM family heme chaperone HemW -
  H1D11_RS06765 rdgB 1467859..1468452 (-) 594 WP_001174747.1 XTP/dITP diphosphatase -
  H1D11_RS06770 yggU 1468460..1468750 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  H1D11_RS06775 yggT 1468747..1469313 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  H1D11_RS06780 yggS 1469331..1470035 (-) 705 WP_001521256.1 pyridoxal phosphate homeostasis protein -
  H1D11_RS06785 pilT 1470053..1471033 (+) 981 WP_115423636.1 type IV pilus twitching motility protein PilT Machinery gene
  H1D11_RS06790 ruvX 1471148..1471564 (-) 417 WP_000017106.1 Holliday junction resolvase RuvX -
  H1D11_RS06795 yqgE 1471564..1472127 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  H1D11_RS06800 gshB 1472236..1473186 (-) 951 WP_000593273.1 glutathione synthase -
  H1D11_RS06805 rsmE 1473199..1473930 (-) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  H1D11_RS06810 endA 1474010..1474717 (-) 708 WP_000286500.1 deoxyribonuclease I -
  H1D11_RS06815 yggI 1474812..1475309 (-) 498 WP_000858396.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36000.16 Da        Isoelectric Point: 5.5901

>NTDB_id=1130078 H1D11_RS06785 WP_115423636.1 1470053..1471033(+) (pilT) [Escherichia coli isolate SC418]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRTILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSDKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSEAIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLVNTPAVANLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=1130078 H1D11_RS06785 WP_115423636.1 1470053..1471033(+) (pilT) [Escherichia coli isolate SC418]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGTATTCGCGGGCGAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGACAATATTGCTGGAGAATGGTCAGCTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGATTGCGCGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCTACGGGGAGCGACAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCCGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGGCAATCCGTCTGGCGCTGACGG
CAGCAGAAACCGGACACCTGGTGCTGGCAACTTTACATACGCGTGGTGCGGCGCAGGCAGTTGAGCGGTTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCGGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTGCTGGTTAACACACCCGCGGTGGCGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGACGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.93

100

0.491
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.93

100

0.491
  pilT Neisseria meningitidis 8013

47.866

100

0.482
  pilT Neisseria gonorrhoeae MS11

47.561

100

0.479
  pilT Acinetobacter baylyi ADP1

45.872

100

0.46
  pilT Acinetobacter nosocomialis M2

45.566

100

0.457
  pilT Acinetobacter baumannii D1279779

45.566

100

0.457
  pilT Acinetobacter baumannii strain A118

45.566

100

0.457
  pilT Pseudomonas stutzeri DSM 10701

45.566

100

0.457
  pilT Pseudomonas aeruginosa PAK

45.26

100

0.454
  pilT Legionella pneumophila strain ERS1305867

44.648

100

0.448
  pilT Legionella pneumophila strain Lp02

44.648

100

0.448
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.509

97.546

0.405
  pilU Vibrio cholerae strain A1552

38.906

100

0.393
  pilU Pseudomonas stutzeri DSM 10701

36.97

100

0.374

Multiple sequence alignment