Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   EL199_RS01620 Genome accession   NZ_LR134365
Coordinates   356315..356755 (+) Length   146 a.a.
NCBI ID   WP_004140956.1    Uniprot ID   C8N9J5
Organism   Cardiobacterium hominis strain NCTC10426     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 351315..361755
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL199_RS01590 (NCTC10426_00311) - 351389..352270 (+) 882 WP_004140966.1 DMT family transporter -
  EL199_RS01595 (NCTC10426_00312) murI 352267..353085 (+) 819 WP_004140965.1 glutamate racemase -
  EL199_RS01600 (NCTC10426_00313) - 353208..353798 (+) 591 WP_004140963.1 Slam-dependent surface lipoprotein -
  EL199_RS01605 (NCTC10426_00314) - 354150..354920 (-) 771 WP_004140962.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  EL199_RS01610 (NCTC10426_00315) - 354994..355482 (-) 489 WP_004140960.1 hypothetical protein -
  EL199_RS01615 (NCTC10426_00316) - 355479..355922 (-) 444 WP_004140958.1 universal stress protein -
  EL199_RS01620 (NCTC10426_00317) pilE 356315..356755 (+) 441 WP_004140956.1 pilin Machinery gene
  EL199_RS01625 pilE 356831..357274 (+) 444 WP_004140954.1 pilin Machinery gene
  EL199_RS01630 - 357338..361138 (+) 3801 WP_081447822.1 fused MFS/spermidine synthase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15397.62 Da        Isoelectric Point: 9.3535

>NTDB_id=1122312 EL199_RS01620 WP_004140956.1 356315..356755(+) (pilE) [Cardiobacterium hominis strain NCTC10426]
MKKLQKGFTLIELMIVIAIIGILAAIALPAYQDYIARSQMTEGFNLAGGQKGALSEFYADKGRWPSNNTEAGIAAASQIT
GKYVANVAVNASQITATMKSSEISKGIQGKTITLTGTVAGNPQDQGSYQWACESNASAKYLPASCR

Nucleotide


Download         Length: 441 bp        

>NTDB_id=1122312 EL199_RS01620 WP_004140956.1 356315..356755(+) (pilE) [Cardiobacterium hominis strain NCTC10426]
ATGAAAAAATTGCAAAAAGGCTTCACCCTCATCGAGCTGATGATCGTTATCGCCATTATCGGTATTCTGGCTGCTATCGC
TCTGCCGGCCTACCAAGATTATATCGCACGTTCACAAATGACCGAAGGTTTTAACCTTGCTGGTGGCCAAAAAGGTGCCC
TGAGCGAATTCTACGCTGACAAAGGCAGATGGCCTTCTAACAATACTGAAGCAGGTATTGCTGCAGCAAGCCAAATTACT
GGCAAATATGTTGCTAACGTTGCTGTTAATGCTAGTCAAATTACAGCCACGATGAAATCTTCTGAAATTTCTAAGGGCAT
TCAAGGTAAAACAATTACCTTGACTGGTACTGTAGCAGGTAACCCGCAGGATCAAGGTTCATATCAGTGGGCTTGCGAAT
CTAACGCTTCTGCTAAATATCTTCCTGCTTCTTGCCGTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(3-28)

Pilin (bacterial filament) (Pilin)

(36-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C8N9J5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae MS11

48.78

100

0.548
  pilE Neisseria gonorrhoeae strain FA1090

48.466

100

0.541
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

38.83

100

0.5
  pilA/pilA1 Eikenella corrodens VA1

45.223

100

0.486
  pilA2 Legionella pneumophila str. Paris

45.833

98.63

0.452
  pilA2 Legionella pneumophila strain ERS1305867

45.139

98.63

0.445
  pilA Ralstonia pseudosolanacearum GMI1000

36.994

100

0.438
  comP Acinetobacter baylyi ADP1

40.132

100

0.418
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.781

100

0.418
  pilA Acinetobacter baumannii strain A118

38.926

100

0.397
  pilA Pseudomonas aeruginosa PAK

37.662

100

0.397
  pilA Acinetobacter nosocomialis M2

44.715

84.247

0.377
  pilA/pilAII Pseudomonas stutzeri DSM 10701

36.242

100

0.37

Multiple sequence alignment