Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   EL199_RS01625 Genome accession   NZ_LR134365
Coordinates   356831..357274 (+) Length   147 a.a.
NCBI ID   WP_004140954.1    Uniprot ID   C8N9J4
Organism   Cardiobacterium hominis strain NCTC10426     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 351831..362274
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL199_RS01595 (NCTC10426_00312) murI 352267..353085 (+) 819 WP_004140965.1 glutamate racemase -
  EL199_RS01600 (NCTC10426_00313) - 353208..353798 (+) 591 WP_004140963.1 Slam-dependent surface lipoprotein -
  EL199_RS01605 (NCTC10426_00314) - 354150..354920 (-) 771 WP_004140962.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  EL199_RS01610 (NCTC10426_00315) - 354994..355482 (-) 489 WP_004140960.1 hypothetical protein -
  EL199_RS01615 (NCTC10426_00316) - 355479..355922 (-) 444 WP_004140958.1 universal stress protein -
  EL199_RS01620 (NCTC10426_00317) pilE 356315..356755 (+) 441 WP_004140956.1 pilin Machinery gene
  EL199_RS01625 pilE 356831..357274 (+) 444 WP_004140954.1 pilin Machinery gene
  EL199_RS01630 - 357338..361138 (+) 3801 WP_081447822.1 fused MFS/spermidine synthase -
  EL199_RS01635 (NCTC10426_00319) tfpZ 361128..361850 (+) 723 WP_004140948.1 TfpX/TfpZ family type IV pilin accessory protein -

Sequence

Protein


Download         Length: 147 a.a.        Molecular weight: 15771.23 Da        Isoelectric Point: 8.9947

>NTDB_id=1122313 EL199_RS01625 WP_004140954.1 356831..357274(+) (pilE) [Cardiobacterium hominis strain NCTC10426]
MGVKVKRNIQQGFTLIELMVVIAIIGVLAAIALPAYQIYVTRAQMGEAFNLVDAQKNAVVETRTNKGDWPSNNTEAGMGD
PTKISGKYVEKVEVTSGGVITATMKSSGIAATIQHKTLTLTPTETNGSYTWECSSNVGKMYLPAVCR

Nucleotide


Download         Length: 444 bp        

>NTDB_id=1122313 EL199_RS01625 WP_004140954.1 356831..357274(+) (pilE) [Cardiobacterium hominis strain NCTC10426]
ATGGGTGTTAAAGTGAAAAGAAATATACAGCAAGGCTTTACGCTGATTGAGTTGATGGTCGTGATTGCTATTATTGGGGT
GTTGGCTGCTATTGCACTTCCCGCTTATCAAATTTATGTTACGCGGGCGCAAATGGGCGAAGCTTTCAATTTAGTGGATG
CGCAAAAGAACGCGGTTGTTGAAACTCGAACCAATAAAGGGGACTGGCCATCTAATAACACAGAAGCAGGTATGGGCGAC
CCTACTAAAATCAGCGGAAAATATGTTGAAAAAGTAGAAGTGACTAGCGGTGGTGTTATTACGGCTACAATGAAATCGTC
TGGAATAGCGGCTACAATACAACATAAAACGTTAACGCTTACTCCTACAGAGACCAATGGTTCATACACTTGGGAATGCA
GTTCTAACGTTGGTAAAATGTATCTTCCTGCAGTATGCAGGTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(41-147)

Prokaryotic N-terminal methylation motif (N_methyl)

(7-33)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C8N9J4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae MS11

49.045

100

0.524
  pilE Neisseria gonorrhoeae strain FA1090

46.795

100

0.497
  pilA2 Legionella pneumophila str. Paris

42.143

95.238

0.401
  pilA2 Legionella pneumophila strain ERS1305867

42.143

95.238

0.401
  pilA/pilA1 Eikenella corrodens VA1

37.255

100

0.388
  comP Acinetobacter baylyi ADP1

37.5

100

0.388
  pilA Acinetobacter baumannii strain A118

38.732

96.599

0.374
  pilA Vibrio cholerae strain A1552

35.256

100

0.374
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

35.256

100

0.374
  pilA Vibrio cholerae C6706

35.256

100

0.374
  pilA/pilAI Pseudomonas stutzeri DSM 10701

37.762

97.279

0.367
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

33.758

100

0.361

Multiple sequence alignment