Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PATL70BA_RS08050 Genome accession   NZ_LR130778
Coordinates   1720589..1721644 (-) Length   351 a.a.
NCBI ID   WP_125136892.1    Uniprot ID   A0A3P7P287
Organism   Petrocella atlantisensis isolate 70B-A     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1715589..1726644
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PATL70BA_RS08025 (PATL70BA_1716) - 1715992..1717191 (-) 1200 WP_125136887.1 S-layer homology domain-containing protein -
  PATL70BA_RS08030 (PATL70BA_1717) - 1717178..1717738 (-) 561 WP_125136888.1 PilN domain-containing protein -
  PATL70BA_RS08035 (PATL70BA_1718) pilM 1717747..1718823 (-) 1077 WP_172596169.1 pilus assembly protein PilM -
  PATL70BA_RS08040 (PATL70BA_1719) - 1718927..1719334 (-) 408 WP_125136890.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  PATL70BA_RS08045 (PATL70BA_1720) - 1719367..1720569 (-) 1203 WP_125136891.1 type II secretion system F family protein -
  PATL70BA_RS08050 (PATL70BA_1721) pilT 1720589..1721644 (-) 1056 WP_125136892.1 type IV pilus twitching motility protein PilT Machinery gene
  PATL70BA_RS08055 (PATL70BA_1722) - 1721657..1723336 (-) 1680 WP_197715798.1 GspE/PulE family protein -
  PATL70BA_RS08060 (PATL70BA_1723) - 1723829..1725205 (-) 1377 WP_172596170.1 anti-sigma factor domain-containing protein -
  PATL70BA_RS08065 (PATL70BA_1724) - 1725210..1725824 (-) 615 WP_125136894.1 sigma-70 family RNA polymerase sigma factor -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 39171.28 Da        Isoelectric Point: 6.8355

>NTDB_id=1117561 PATL70BA_RS08050 WP_125136892.1 1720589..1721644(-) (pilT) [Petrocella atlantisensis isolate 70B-A]
MDIQGVLEEGLKRRASDVHLKVGSPPILRVNGDLLPIGDDIITGENTYQLLKSMLSISQFETLESRGEFDFSYSVPGIGR
YRVNGYRQRDSYCLAMRMVNFEIPTLESLGIPGSVEVLTELKNGLVLITGPTGSGKSTTLAAMIERINQTRRTHILTLED
PIEYLFHNKKSIIGQREIGSDSHSFKDALRATLRQDPDIILVGEMRDLETIEIALTAAETGHLVLSTLHTVGAPKTIDRI
IDVFPQNSKDQIRVQVASVLQGVVSQQLLPIKSGMGRVPACEILIPTMAIRNLIREKKNHQIINAIQTGRHLGMMTMDHS
LRDLFRQGLITREVAIEYAFDKEELIKTLVH

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=1117561 PATL70BA_RS08050 WP_125136892.1 1720589..1721644(-) (pilT) [Petrocella atlantisensis isolate 70B-A]
ATGGATATACAAGGCGTATTAGAAGAAGGTCTAAAAAGAAGAGCCTCAGATGTTCACTTGAAAGTAGGCTCGCCACCCAT
ACTCCGGGTCAATGGAGACTTGTTGCCTATTGGTGACGACATAATAACTGGTGAGAACACCTATCAACTCCTTAAGTCCA
TGCTCTCAATAAGTCAGTTTGAGACATTAGAAAGCCGGGGGGAATTTGATTTTTCCTATTCTGTACCGGGCATTGGCAGG
TACAGGGTTAACGGTTATCGGCAAAGAGACAGTTATTGTTTGGCCATGCGTATGGTTAATTTTGAGATTCCCACATTGGA
ATCACTGGGTATTCCAGGTAGTGTAGAAGTCCTGACAGAACTTAAAAACGGTCTGGTCCTCATAACTGGACCAACAGGCA
GTGGTAAGTCCACAACTCTAGCAGCTATGATTGAGAGAATCAACCAGACCAGAAGAACCCATATACTCACTCTAGAAGAC
CCAATCGAGTATCTGTTTCATAACAAGAAAAGCATCATCGGACAGAGAGAAATCGGCTCCGACTCTCATAGCTTTAAGGA
TGCCCTAAGGGCCACATTACGACAGGACCCGGATATTATTCTAGTAGGTGAGATGCGAGATTTGGAAACCATAGAGATAG
CCCTAACAGCAGCAGAAACCGGCCATCTGGTTCTAAGCACTCTACATACCGTAGGGGCACCCAAAACCATCGACCGTATC
ATCGATGTCTTTCCACAAAACAGCAAAGATCAGATTCGTGTTCAAGTGGCCTCTGTCTTACAAGGGGTGGTATCTCAGCA
GTTGTTACCAATCAAGTCAGGTATGGGTCGTGTACCCGCATGTGAGATTCTTATTCCAACCATGGCTATTAGAAATCTCA
TTCGTGAGAAAAAGAACCACCAGATCATCAACGCCATTCAGACAGGACGCCATCTTGGCATGATGACCATGGACCATTCT
CTAAGAGATCTCTTTAGACAAGGGTTGATTACAAGAGAAGTCGCCATAGAATACGCCTTTGACAAAGAAGAACTCATTAA
GACCTTAGTACATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3P7P287

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.856

98.86

0.493
  pilT Pseudomonas stutzeri DSM 10701

49.275

98.291

0.484
  pilT Acinetobacter baylyi ADP1

48.986

98.291

0.481
  pilT Pseudomonas aeruginosa PAK

49.853

96.581

0.481
  pilT Acinetobacter baumannii strain A118

48.555

98.575

0.479
  pilT Acinetobacter baumannii D1279779

48.555

98.575

0.479
  pilT Acinetobacter nosocomialis M2

48.555

98.575

0.479
  pilT Neisseria meningitidis 8013

48.406

98.291

0.476
  pilT Neisseria gonorrhoeae MS11

47.826

98.291

0.47
  pilT Legionella pneumophila strain Lp02

47.076

97.436

0.459
  pilT Legionella pneumophila strain ERS1305867

47.076

97.436

0.459
  pilT Vibrio cholerae strain A1552

48.485

94.017

0.456
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.485

94.017

0.456
  pilU Pseudomonas stutzeri DSM 10701

38.551

98.291

0.379
  pilU Vibrio cholerae strain A1552

38.551

98.291

0.379

Multiple sequence alignment