Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   VANGNB10_RS07525 Genome accession   NZ_LK021130
Coordinates   404967..406073 (+) Length   368 a.a.
NCBI ID   WP_017045509.1    Uniprot ID   A0AAW4AIC1
Organism   Vibrio anguillarum strain NB10     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 399967..411073
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VANGNB10_RS07490 (TVA2239) - 400316..400915 (-) 600 WP_013857824.1 XTP/dITP diphosphatase -
  VANGNB10_RS07495 (TVA2238) - 400928..401359 (-) 432 WP_013857823.1 DUF4426 domain-containing protein -
  VANGNB10_RS07500 (TVA2237) yggU 401402..401692 (-) 291 WP_040122938.1 DUF167 family protein YggU -
  VANGNB10_RS07505 (TVA2236) - 401692..402249 (-) 558 WP_010320643.1 YggT family protein -
  VANGNB10_RS07510 (TVA2235) proC 402312..403130 (-) 819 WP_040122939.1 pyrroline-5-carboxylate reductase -
  VANGNB10_RS07515 (TVA2234) - 403192..403893 (-) 702 WP_013857820.1 YggS family pyridoxal phosphate-dependent enzyme -
  VANGNB10_RS07520 (TVA2233) pilT 403919..404956 (+) 1038 WP_013857819.1 type IV pilus twitching motility protein PilT Machinery gene
  VANGNB10_RS07525 (TVA2232) pilU 404967..406073 (+) 1107 WP_017045509.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  VANGNB10_RS07530 (TVA2231) ruvX 406157..406585 (-) 429 WP_013857817.1 Holliday junction resolvase RuvX -
  VANGNB10_RS07535 (TVA2230) - 406671..407234 (-) 564 WP_013857816.1 YqgE/AlgH family protein -
  VANGNB10_RS07540 (TVA2229) gshB 407281..408234 (-) 954 WP_040122940.1 glutathione synthase -
  VANGNB10_RS07545 (TVA2228) rsmE 408248..408979 (-) 732 WP_013857814.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  VANGNB10_RS07550 (TVA2227) endA 409088..409795 (-) 708 WP_013857813.1 deoxyribonuclease I -
  VANGNB10_RS07555 (TVA2226) - 409927..410427 (-) 501 WP_013857812.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41399.46 Da        Isoelectric Point: 6.5071

>NTDB_id=1113421 VANGNB10_RS07525 WP_017045509.1 404967..406073(+) (pilU) [Vibrio anguillarum strain NB10]
MELNQCLEGMLTQKASDLYLTVGAPVLFRVDGELRAHGEKLSQTQVMALLDAMMDEDRRREFQRTREANFAVVREIGRFR
VSAFFQRELPGAVIRRIETHIPTFEELKLPGVLQDLSIAKRGLVLVVGATGSGKSTTMAAMTGYRNSHRTGHILTVEDPI
EFVHEHQRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLKGVVGQQLIRDKNGKGRHGVFEILLNSPRISDLIRRGDLHELKATMGKSKEVGMQTFDQALY
QLVVDDKISEQDALHSADSANDLRLMLKTKRGEGISSGTLDNVQIDME

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=1113421 VANGNB10_RS07525 WP_017045509.1 404967..406073(+) (pilU) [Vibrio anguillarum strain NB10]
ATGGAACTGAACCAATGTCTTGAGGGTATGTTGACTCAAAAAGCTTCCGACTTGTACCTCACCGTAGGAGCGCCAGTGTT
ATTTCGCGTCGATGGAGAGTTAAGAGCTCATGGCGAAAAGTTATCCCAAACGCAGGTGATGGCGTTGTTAGATGCCATGA
TGGATGAGGATCGCCGCCGTGAATTTCAACGCACACGTGAAGCCAATTTTGCAGTGGTGAGAGAGATTGGCCGTTTTCGT
GTCAGTGCTTTTTTTCAGCGTGAGCTGCCCGGTGCGGTGATCCGCCGTATTGAAACTCATATCCCCACTTTTGAAGAATT
AAAGTTACCGGGCGTGTTGCAAGATCTCTCGATTGCAAAGCGTGGGCTGGTGCTGGTGGTAGGCGCGACGGGGTCGGGCA
AATCGACCACCATGGCGGCCATGACGGGTTATCGTAACTCGCACCGAACAGGGCATATTTTAACGGTGGAAGATCCGATT
GAGTTTGTGCATGAGCATCAGCGTTGCATTGTGACCCAGCGAGAAGTGGGGTTGGATACCGAGAGTTACGAAGTGGCGCT
GAAAAACTCGCTGCGCCAAGCCCCTGATATGATTTTAATTGGTGAGATCCGTTCACGTGAAACGATGGAATATGCGATGA
CCTTTGCCGAGACAGGTCATTTATGTATGGCAACGTTACATGCTAACAATGCCAACCAAGCGCTTGAGCGAATTTTGCAT
TTGGTTCCTAAAGAGCAAAAAGAGCAGTTTTTGTTTGATCTGTCGATGAACCTCAAAGGCGTCGTGGGGCAGCAGTTGAT
CCGCGATAAAAATGGCAAGGGACGACATGGTGTGTTTGAGATTTTACTCAACAGCCCTCGAATTTCCGATTTGATCCGCC
GCGGTGATCTGCACGAATTGAAAGCGACGATGGGCAAATCAAAAGAAGTGGGGATGCAGACTTTTGACCAAGCACTGTAT
CAACTGGTCGTTGATGACAAAATCAGTGAACAAGATGCACTGCACAGCGCTGACTCGGCTAACGATTTGCGTTTAATGTT
AAAAACAAAGCGTGGCGAAGGGATCAGCAGTGGCACATTGGATAACGTGCAGATTGATATGGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

87.5

100

0.875
  pilU Pseudomonas stutzeri DSM 10701

58.286

95.109

0.554
  pilU Acinetobacter baylyi ADP1

53.561

95.38

0.511
  pilT Pseudomonas aeruginosa PAK

42.478

92.12

0.391
  pilT Acinetobacter baylyi ADP1

41.935

92.663

0.389
  pilT Legionella pneumophila strain ERS1305867

41.888

92.12

0.386
  pilT Legionella pneumophila strain Lp02

41.888

92.12

0.386
  pilT Acinetobacter nosocomialis M2

41.593

92.12

0.383
  pilT Acinetobacter baumannii D1279779

41.593

92.12

0.383
  pilT Acinetobacter baumannii strain A118

41.593

92.12

0.383
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.192

84.783

0.383
  pilT Pseudomonas stutzeri DSM 10701

41.003

92.12

0.378
  pilT Vibrio cholerae strain A1552

40.719

90.761

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.719

90.761

0.37

Multiple sequence alignment