Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CSG_RS04255 Genome accession   NZ_HG004426
Coordinates   837942..839003 (+) Length   353 a.a.
NCBI ID   WP_002849776.1    Uniprot ID   A0A5L8UCG3
Organism   Campylobacter fetus subsp. venerealis str. 84-112     
Function   pseudopilus biogenesis (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 832942..844003
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CSG_RS04220 (CSG_8410) brnQ 832995..834299 (-) 1305 WP_024305202.1 branched-chain amino acid transport system II carrier protein -
  CSG_RS04225 (CSG_8420) hisG 834308..834919 (-) 612 WP_010400265.1 ATP phosphoribosyltransferase -
  CSG_RS04230 (CSG_8430) - 834907..835539 (-) 633 WP_002849782.1 type III pantothenate kinase -
  CSG_RS04235 (CSG_8440) - 835526..835852 (-) 327 WP_002849781.1 hypothetical protein -
  CSG_RS04240 (CSG_8450) - 835859..836866 (-) 1008 WP_002849780.1 hypothetical protein -
  CSG_RS04245 (CSG_8460) - 836869..837468 (-) 600 WP_230454695.1 hypothetical protein -
  CSG_RS04250 (CSG_8470) gatC 837651..837938 (+) 288 WP_002849777.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC -
  CSG_RS04255 (CSG_8480) pilT 837942..839003 (+) 1062 WP_002849776.1 type IV pilus twitching motility protein PilT Machinery gene
  CSG_RS04260 (CSG_8490) - 839003..839578 (+) 576 WP_002849774.1 CvpA family protein -
  CSG_RS04265 (CSG_8500) - 839582..840046 (+) 465 WP_002849768.1 Fur family transcriptional regulator -
  CSG_RS04270 (CSG_8510) lysS 840060..841571 (+) 1512 WP_024305203.1 lysine--tRNA ligase -
  CSG_RS04275 (CSG_8520) - 841568..842812 (+) 1245 WP_002849766.1 serine hydroxymethyltransferase -
  CSG_RS04280 (CSG_8530) - 842812..843354 (+) 543 WP_002849765.1 DUF1882 domain-containing protein -

Sequence

Protein


Download         Length: 353 a.a.        Molecular weight: 38935.38 Da        Isoelectric Point: 6.3450

>NTDB_id=1111831 CSG_RS04255 WP_002849776.1 837942..839003(+) (pilT) [Campylobacter fetus subsp. venerealis str. 84-112]
MNIETLLKTVMHNKASDLHLVGRSEPQIRIDGSLRPLEGKVLDGSDIENLCYTILTDAQKSELEENKDLDFALELPGIGR
FRGNCYYTMNGSLAAAFRMIPLNIPSLDDLNAPAIFKELVKKEKGLVLVTGGTGAGKSTTLAALLNEINITERKHIITIE
DPIEFVHQNKNSLFSHRNVGTDTKSFARALKYALREDPDIILVGDLRDPETISIAIAAAETGHLVFGTLHTNSAVQTINR
IVDSFNETEQAQVRNMLSVSLNAIISQTLVPKADNGRFAVHEVLINNHAISNLIRENKSHQIYSQIQLNQQNTGMQTQTQ
AMEKAVQMGIITKENAIRYSTNQQELAGKLGAI

Nucleotide


Download         Length: 1062 bp        

>NTDB_id=1111831 CSG_RS04255 WP_002849776.1 837942..839003(+) (pilT) [Campylobacter fetus subsp. venerealis str. 84-112]
ATGAATATTGAAACTCTTTTAAAAACTGTGATGCATAACAAGGCGAGCGATCTACATTTAGTCGGTAGAAGTGAGCCTCA
AATCAGAATTGATGGTAGCTTAAGACCGCTTGAAGGTAAAGTTCTAGATGGAAGCGATATAGAAAATTTATGCTACACTA
TTTTAACTGATGCGCAAAAAAGCGAATTGGAAGAGAACAAGGATTTAGATTTTGCTCTAGAATTACCAGGAATAGGGCGT
TTTAGAGGTAATTGCTACTATACTATGAACGGAAGCTTAGCGGCTGCTTTTAGAATGATACCTCTAAATATACCAAGTCT
TGATGATCTTAATGCTCCGGCTATTTTTAAAGAGTTGGTAAAAAAAGAAAAAGGTCTTGTTCTTGTTACTGGAGGAACGG
GAGCTGGAAAATCGACGACTTTAGCAGCTTTGCTAAATGAGATAAATATTACTGAGCGAAAACATATAATCACCATAGAA
GATCCTATAGAATTTGTTCATCAAAATAAGAATTCTCTATTTTCTCATAGAAACGTAGGCACCGATACTAAATCGTTTGC
TAGGGCTTTGAAATACGCTCTTAGAGAAGATCCGGATATAATATTAGTAGGTGATTTAAGAGATCCGGAGACCATATCTA
TAGCTATAGCGGCCGCAGAAACAGGACACTTGGTATTTGGTACTTTGCACACAAACTCAGCCGTACAAACAATAAATAGA
ATAGTCGATAGTTTTAATGAGACAGAGCAGGCTCAAGTAAGAAATATGCTTAGCGTATCTCTAAATGCTATTATATCTCA
AACTCTTGTGCCAAAAGCAGATAACGGCAGATTTGCCGTTCATGAAGTTTTGATAAATAATCATGCAATTTCAAATTTGA
TCAGAGAAAATAAATCTCATCAAATTTACTCTCAAATACAATTAAATCAACAAAATACAGGTATGCAAACACAAACTCAA
GCGATGGAAAAGGCGGTGCAAATGGGTATTATAACAAAAGAAAACGCTATAAGATATTCAACAAATCAGCAAGAATTAGC
TGGAAAGTTAGGTGCAATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5L8UCG3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria gonorrhoeae MS11

50.287

98.584

0.496
  pilT Legionella pneumophila strain Lp02

50.729

97.167

0.493
  pilT Legionella pneumophila strain ERS1305867

50.729

97.167

0.493
  pilT Neisseria meningitidis 8013

50

98.584

0.493
  pilT Vibrio cholerae strain A1552

51.964

93.768

0.487
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.964

93.768

0.487
  pilT Acinetobacter baumannii D1279779

49.565

97.734

0.484
  pilT Acinetobacter nosocomialis M2

49.565

97.734

0.484
  pilT Acinetobacter baumannii strain A118

49.565

97.734

0.484
  pilT Pseudomonas aeruginosa PAK

49.702

95.184

0.473
  pilT Acinetobacter baylyi ADP1

50.151

93.768

0.47
  pilT Pseudomonas stutzeri DSM 10701

48.81

95.184

0.465
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.092

98.584

0.405
  pilU Pseudomonas stutzeri DSM 10701

40

100

0.402
  pilU Acinetobacter baylyi ADP1

39.181

96.884

0.38

Multiple sequence alignment