Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACKTT5_RS19035 Genome accession   NZ_AP038761
Coordinates   3915800..3916780 (-) Length   326 a.a.
NCBI ID   WP_003860028.1    Uniprot ID   -
Organism   Enterobacter hormaechei subsp. xiangfangensis strain B12-S377     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3910800..3921780
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKTT5_RS19005 - 3911681..3912178 (+) 498 WP_003860035.1 SprT family zinc-dependent metalloprotease -
  ACKTT5_RS19010 endA 3912273..3912980 (+) 708 WP_003860034.1 deoxyribonuclease I -
  ACKTT5_RS19015 rsmE 3913032..3913763 (+) 732 WP_048242066.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACKTT5_RS19020 gshB 3913791..3914738 (+) 948 WP_003860032.1 glutathione synthase -
  ACKTT5_RS19025 - 3914813..3915373 (+) 561 WP_022649353.1 YqgE/AlgH family protein -
  ACKTT5_RS19030 ruvX 3915373..3915789 (+) 417 WP_003860030.1 Holliday junction resolvase RuvX -
  ACKTT5_RS19035 pilT 3915800..3916780 (-) 981 WP_003860028.1 type IV pilus twitching motility protein PilT Machinery gene
  ACKTT5_RS19040 - 3916798..3917499 (+) 702 WP_003860026.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACKTT5_RS19045 - 3917521..3918087 (+) 567 WP_003860024.1 YggT family protein -
  ACKTT5_RS19050 yggU 3918084..3918380 (+) 297 WP_003860023.1 DUF167 family protein YggU -
  ACKTT5_RS19055 - 3918384..3918977 (+) 594 WP_003860021.1 XTP/dITP diphosphatase -
  ACKTT5_RS19060 hemW 3918970..3920118 (+) 1149 WP_048242068.1 radical SAM family heme chaperone HemW -
  ACKTT5_RS19065 - 3920175..3920537 (+) 363 WP_048210608.1 endonuclease domain-containing protein -
  ACKTT5_RS19070 - 3920650..3921366 (-) 717 WP_003862419.1 DUF2884 domain-containing protein -
  ACKTT5_RS19075 - 3921424..3921750 (-) 327 WP_003862421.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35622.60 Da        Isoelectric Point: 6.2335

>NTDB_id=111128 ACKTT5_RS19035 WP_003860028.1 3915800..3916780(-) (pilT) [Enterobacter hormaechei subsp. xiangfangensis strain B12-S377]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVEALLKAWLNDEQQGAWWANGQVDFAVTLTGGQRLRG
SAFKHMHGVSIALRLLPLTCPQLSALGVPRAIPELLSNDNGLILVTGATGSGKSTTLAAMVDFLNHQTDGHILTLEDPVE
FMYQSERCLIQQREIGLHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLRQDVQGGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=111128 ACKTT5_RS19035 WP_003860028.1 3915800..3916780(-) (pilT) [Enterobacter hormaechei subsp. xiangfangensis strain B12-S377]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCACCACCGCG
CTGGCGCAGGTCAGGCCGTCTTGAACCTGCGCCGTTTCCGCCCCCGGATGTGGAGGCGTTATTAAAAGCGTGGCTCAATG
ACGAACAGCAGGGCGCCTGGTGGGCAAACGGGCAGGTGGATTTTGCCGTTACCCTCACAGGCGGTCAGCGGCTGCGCGGC
AGTGCGTTTAAGCATATGCACGGCGTTTCGATCGCGCTGCGCCTGTTGCCGCTGACGTGCCCGCAGCTCTCTGCGTTAGG
CGTGCCGCGCGCGATCCCGGAGCTTTTGTCCAATGACAATGGCCTGATTCTGGTCACCGGCGCCACCGGCAGCGGGAAAT
CGACCACTCTGGCCGCGATGGTCGATTTTCTCAATCACCAGACAGACGGGCACATTCTGACCCTGGAAGATCCGGTGGAG
TTTATGTACCAGAGTGAACGTTGCCTGATCCAGCAGCGGGAGATAGGCCTGCACAGCCCGTCCTTTGCCGAGGCGCTGCG
CAGCGCGCTGCGTGAAGATCCGGATGTAATTCTGCTTGGTGAGCTGCGCGACAGCGAGACGATACGCCTGGCGCTGACGG
CGGCGGAAACCGGCCATCTGGTGTTGGCCACGCTGCACACGCGCGGTGCGGCGCAGGCGATCGAGCGCCTGGTCGACACC
TTTCCGGCGCAGGAGAAAGATCCGGTGCGTAATCAGCTGGCGGGAAGCCTGCGTGCGGTTCTGGCGCAGAAGCTGCGTCA
GGATGTCCAGGGCGGGCGCGTGGCGCTGTATGAGCTGCTGGTGAATACCTCGGCGGCGGCAAACCTGATCCGCGAAGGCA
AAACCTGGCAACTGCCGGGCATTATTCAAACGGGGCAGCAGGCGGGAATGCAGAACTTTGAGCAGAGTCTGGCGGAGCGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(75-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Pseudomonas stutzeri DSM 10701

48.012

100

0.482
  pilT Legionella pneumophila strain Lp02

48.447

98.773

0.479
  pilT Legionella pneumophila strain ERS1305867

48.447

98.773

0.479
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Acinetobacter baumannii D1279779

47.095

100

0.472
  pilT Acinetobacter baumannii strain A118

47.095

100

0.472
  pilT Pseudomonas aeruginosa PAK

47.095

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

47.5

98.16

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.344

99.08

0.429
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.387
  pilU Vibrio cholerae strain A1552

38.509

98.773

0.38

Multiple sequence alignment