Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACN079_RS24680 Genome accession   NZ_CP184478
Coordinates   5502399..5502818 (-) Length   139 a.a.
NCBI ID   WP_420232241.1    Uniprot ID   -
Organism   Pseudomonas sp. ABY48     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5497399..5507818
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACN079_RS24665 (ACN079_24665) - 5498107..5498928 (+) 822 WP_420232238.1 IclR family transcriptional regulator -
  ACN079_RS24670 (ACN079_24670) - 5499050..5501602 (-) 2553 WP_420232239.1 LuxR C-terminal-related transcriptional regulator -
  ACN079_RS24675 (ACN079_24675) - 5501774..5502127 (-) 354 WP_420232240.1 BON domain-containing protein -
  ACN079_RS24680 (ACN079_24680) pilA 5502399..5502818 (-) 420 WP_420232241.1 pilin Machinery gene
  ACN079_RS24685 (ACN079_24685) pilB 5503042..5504742 (+) 1701 WP_420232242.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACN079_RS24690 (ACN079_24690) pilC 5504745..5505962 (+) 1218 WP_420232243.1 type II secretion system F family protein Machinery gene
  ACN079_RS24695 (ACN079_24695) pilD 5505964..5506833 (+) 870 WP_420232244.1 prepilin peptidase Machinery gene
  ACN079_RS24700 (ACN079_24700) coaE 5506833..5507456 (+) 624 WP_420232245.1 dephospho-CoA kinase -
  ACN079_RS24705 (ACN079_24705) yacG 5507453..5507653 (+) 201 WP_058542677.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 139 a.a.        Molecular weight: 13989.08 Da        Isoelectric Point: 7.5917

>NTDB_id=1108430 ACN079_RS24680 WP_420232241.1 5502399..5502818(-) (pilA) [Pseudomonas sp. ABY48]
MNKQSGFTLIELLIVVAIIGILATFALPQYSKYQARAKATAGLAEVTALKVPFEDVMNQGTNPTLANIGGTSPTSNCTLT
ASGTAADGAGTIACTLLNAPAPVLGKAITLTRSSQGVWTCGSTAAQEFLPAGCTGTATP

Nucleotide


Download         Length: 420 bp        

>NTDB_id=1108430 ACN079_RS24680 WP_420232241.1 5502399..5502818(-) (pilA) [Pseudomonas sp. ABY48]
ATGAATAAGCAGAGTGGTTTTACCTTGATTGAGCTGCTGATCGTCGTGGCGATCATCGGCATTCTGGCGACCTTTGCGTT
GCCGCAGTATTCCAAGTATCAGGCGCGGGCCAAGGCCACGGCTGGGCTGGCGGAGGTCACTGCATTGAAAGTGCCGTTCG
AAGACGTCATGAATCAAGGCACCAATCCAACGTTGGCCAATATCGGCGGGACCTCGCCCACCAGCAACTGCACCTTGACG
GCTTCGGGAACCGCGGCGGACGGAGCGGGCACTATCGCATGCACGCTCTTGAATGCGCCCGCTCCGGTACTTGGCAAAGC
GATCACCCTGACGCGTTCCTCTCAAGGCGTTTGGACCTGCGGTTCGACCGCCGCTCAGGAATTCCTGCCAGCGGGTTGCA
CAGGTACAGCGACTCCGTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

49.275

99.281

0.489
  pilA/pilAI Pseudomonas stutzeri DSM 10701

47.445

98.561

0.468
  pilA/pilAII Pseudomonas stutzeri DSM 10701

43.284

96.403

0.417
  pilA Pseudomonas aeruginosa PAK

38.926

100

0.417
  pilA2 Legionella pneumophila strain ERS1305867

39.31

100

0.41
  pilA2 Legionella pneumophila str. Paris

39.31

100

0.41
  pilA Vibrio cholerae strain A1552

37.415

100

0.396
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

37.415

100

0.396
  pilA Vibrio cholerae C6706

37.415

100

0.396
  comP Acinetobacter baylyi ADP1

35.57

100

0.381
  pilA Ralstonia pseudosolanacearum GMI1000

33.548

100

0.374
  pilA Vibrio parahaemolyticus RIMD 2210633

39.844

92.086

0.367

Multiple sequence alignment