Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACN079_RS24685 Genome accession   NZ_CP184478
Coordinates   5503042..5504742 (+) Length   566 a.a.
NCBI ID   WP_420232242.1    Uniprot ID   -
Organism   Pseudomonas sp. ABY48     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5498042..5509742
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACN079_RS24665 (ACN079_24665) - 5498107..5498928 (+) 822 WP_420232238.1 IclR family transcriptional regulator -
  ACN079_RS24670 (ACN079_24670) - 5499050..5501602 (-) 2553 WP_420232239.1 LuxR C-terminal-related transcriptional regulator -
  ACN079_RS24675 (ACN079_24675) - 5501774..5502127 (-) 354 WP_420232240.1 BON domain-containing protein -
  ACN079_RS24680 (ACN079_24680) pilA 5502399..5502818 (-) 420 WP_420232241.1 pilin Machinery gene
  ACN079_RS24685 (ACN079_24685) pilB 5503042..5504742 (+) 1701 WP_420232242.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACN079_RS24690 (ACN079_24690) pilC 5504745..5505962 (+) 1218 WP_420232243.1 type II secretion system F family protein Machinery gene
  ACN079_RS24695 (ACN079_24695) pilD 5505964..5506833 (+) 870 WP_420232244.1 prepilin peptidase Machinery gene
  ACN079_RS24700 (ACN079_24700) coaE 5506833..5507456 (+) 624 WP_420232245.1 dephospho-CoA kinase -
  ACN079_RS24705 (ACN079_24705) yacG 5507453..5507653 (+) 201 WP_058542677.1 DNA gyrase inhibitor YacG -
  ACN079_RS24710 (ACN079_24710) - 5507800..5508015 (-) 216 WP_210640988.1 hypothetical protein -
  ACN079_RS24715 (ACN079_24715) - 5508079..5508765 (-) 687 WP_420232246.1 energy-coupling factor ABC transporter permease -
  ACN079_RS24720 (ACN079_24720) - 5508909..5509535 (+) 627 WP_245140946.1 DUF1780 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62678.78 Da        Isoelectric Point: 5.3203

>NTDB_id=1108431 ACN079_RS24685 WP_420232242.1 5503042..5504742(+) (pilB) [Pseudomonas sp. ABY48]
MNDIALSGLTKQLVLAELLTEQSAQQAYQQAQRSRIPLVSYLVQNKLVQSRQIAEIASEHFGVALLDLNSLDKEAQPSGL
VSEKLVRQHHALPLWRRGNKLFVGISDPTNHQVINDIQFSTGLTTEAILVEDDKLSDAIEKFFESSSTGLEGMGDVDLDG
LDIESIDDTRQDSIAGQDTDDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRVRTDGILREVARPPIQLANRIAARL
KVMASLDISERRKPQDGRLKMRLSKTKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPDQKDLYMAALKQPQG
LILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLIRLQNMGIPGFNIATAVHLIIAQRLARKLCSHCKKAIEIPEETLLKEGFPR
ERIGTFTIYEPVGCEQCNLGYKGRVGVYEVVKNTPELQRLIMAEGNSLEIDLQMRKDGFNDLRASGLLKVMQGVTSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=1108431 ACN079_RS24685 WP_420232242.1 5503042..5504742(+) (pilB) [Pseudomonas sp. ABY48]
ATGAATGACATCGCCCTCAGCGGCCTGACCAAGCAATTGGTGTTGGCCGAACTGCTCACCGAGCAAAGTGCGCAACAGGC
GTATCAACAGGCCCAACGCAGTCGCATCCCCCTGGTCAGCTACCTGGTGCAGAACAAACTGGTGCAGAGCCGCCAGATCG
CGGAGATCGCCTCGGAGCATTTTGGCGTTGCCCTATTGGACCTCAACAGCCTGGACAAAGAAGCCCAGCCCAGCGGCCTG
GTTAGCGAAAAACTGGTGCGCCAGCATCACGCCCTGCCGCTGTGGCGGCGCGGCAACAAGTTGTTCGTCGGCATCTCCGA
CCCCACCAATCACCAGGTCATCAACGACATCCAATTCAGCACCGGCCTGACCACCGAAGCCATCCTGGTGGAGGACGACA
AGCTCAGCGACGCCATCGAAAAGTTTTTCGAGTCCAGCAGCACCGGCCTGGAGGGCATGGGCGATGTCGACCTCGACGGC
CTGGACATCGAATCGATCGACGACACCAGGCAGGATTCCATCGCCGGCCAGGACACCGACGATGCGCCCGTGGTGCGCTT
CGTCAACAAGATGCTGCTGGACGCGATCAAGGGCGGCTCTTCCGACCTGCACTTTGAGCCCTACGAAAAAATCTACCGGG
TGCGAGTGCGCACCGACGGCATATTGCGCGAAGTGGCCAGGCCGCCGATCCAGTTGGCCAACCGTATCGCCGCACGCCTG
AAAGTCATGGCCAGCCTGGATATTTCCGAGCGGCGCAAACCCCAGGACGGACGCCTGAAAATGCGCCTGTCGAAAACCAA
GTCCATCGATTTCCGGGTCAACACCCTGCCCACGCTCTGGGGCGAAAAAGTGGTGATCCGGATCCTCGACCCGTCCAGTG
CGCAAATGGGCATCGATGCCCTGGGCTACGAACCGGACCAGAAAGACCTGTACATGGCCGCCCTCAAGCAACCCCAAGGG
CTGATCCTGGTGACCGGCCCGACCGGCTCGGGCAAGACCGTGTCGCTGTACACCGGGCTGAATATCCTCAACACCGTGGA
CATCAACATTTCCACCGCCGAAGACCCGGTGGAGATCAACATGGAAGGCATCAACCAGGTCAATGTGAACCCGCGCCAGG
GGCTGGATTTCGCCCAGGCCCTGCGCTCGTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTG
GAAACCGCCGAAATCGCCATCAAGGCCGCCCAGACCGGGCACCTGGTGCTCTCCACCCTGCACACCAACAGCGCCGCCGA
AACCCTGATCCGCCTGCAGAACATGGGCATCCCGGGGTTCAACATCGCCACCGCCGTGCACCTGATCATCGCCCAACGGC
TGGCGCGCAAGCTGTGCAGTCACTGCAAGAAGGCCATCGAGATTCCCGAGGAAACCTTGCTCAAGGAAGGCTTCCCCCGG
GAACGCATCGGCACATTCACGATCTATGAGCCGGTCGGTTGCGAACAGTGCAACCTTGGTTACAAAGGGCGCGTGGGGGT
TTATGAAGTGGTCAAGAACACGCCCGAGCTGCAACGGTTGATCATGGCCGAGGGCAACTCGCTGGAAATCGATCTGCAAA
TGCGCAAGGACGGTTTCAATGACCTGCGCGCGTCGGGGCTGCTCAAAGTGATGCAGGGCGTCACCAGCCTCGAAGAAATC
AACCGGGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.127

100

0.581
  pilB Acinetobacter baumannii D1279779

57.496

100

0.576
  pilB Legionella pneumophila strain ERS1305867

55.379

100

0.555
  pilB Vibrio cholerae strain A1552

50.442

99.823

0.504
  pilB Vibrio campbellii strain DS40M4

48.596

100

0.489
  pilF Neisseria gonorrhoeae MS11

48.49

99.47

0.482
  pilB Vibrio parahaemolyticus RIMD 2210633

48.921

98.233

0.481

Multiple sequence alignment