Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   D3W47_RS03055 Genome accession   NZ_CP182821
Coordinates   599572..600849 (-) Length   425 a.a.
NCBI ID   WP_119673882.1    Uniprot ID   A0A418QGB7
Organism   Deinococcus sp. RM     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 594572..605849
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D3W47_RS03025 (D3W47_003025) - 594925..595461 (-) 537 WP_119673888.1 DinB family protein -
  D3W47_RS03030 (D3W47_003030) - 595563..596132 (-) 570 WP_119673887.1 DinB family protein -
  D3W47_RS03035 (D3W47_003035) - 596136..596627 (-) 492 WP_119673886.1 DinB family protein -
  D3W47_RS03040 (D3W47_003040) - 596714..597703 (+) 990 WP_119673885.1 helix-turn-helix transcriptional regulator -
  D3W47_RS03045 (D3W47_003045) - 597700..598149 (+) 450 WP_119673884.1 (4Fe-4S)-binding protein -
  D3W47_RS03050 (D3W47_003050) - 598190..599491 (+) 1302 WP_199699837.1 thymidine phosphorylase -
  D3W47_RS03055 (D3W47_003055) pilT 599572..600849 (-) 1278 WP_119673882.1 type IV pilus twitching motility protein PilT Machinery gene
  D3W47_RS03060 (D3W47_003060) pilB 600895..603573 (-) 2679 WP_119673881.1 type II/IV secretion system protein Machinery gene
  D3W47_RS03065 (D3W47_003065) - 603628..604155 (-) 528 WP_058978804.1 YqeG family HAD IIIA-type phosphatase -
  D3W47_RS03070 (D3W47_003070) pgeF 604152..604880 (-) 729 WP_233555254.1 peptidoglycan editing factor PgeF -
  D3W47_RS03075 (D3W47_003075) - 605017..605811 (+) 795 WP_119673879.1 enoyl-ACP reductase FabI -

Sequence

Protein


Download         Length: 425 a.a.        Molecular weight: 45995.26 Da        Isoelectric Point: 7.0810

>NTDB_id=1102634 D3W47_RS03055 WP_119673882.1 599572..600849(-) (pilT) [Deinococcus sp. RM]
MTQPAADITDILRFAADKGASDVIITVGLSPQFKLQGVYDSQGFAELAPTDTRKLMYSMMNEKQQRTFEERRELDFSFAL
GEKARFRVNAFMQRGNVGGVLRLIPTKIKSAQEMGLPANVIEIANSPRGLVLVTGPTGSGKSTTLAAMIDHINTTKRLHI
MTIEDPIEFMHTHKQSIINQREVGADTMSFNDALRAVLRQAPDVILVGEMRDYETIKAAVTAAETGHLVMGTLHTNSAPE
SIDRIVDVFPEEQQEQIRVQLANNLVAVMTQQLLPRLDGQGRILAYELLIANPAVRALIREGKTYQITSVMQTGAREGMV
TMDAFLANLYRRRVISFDTGVERAVDSKEFARLANDPSIGTAGGAASMPAGYGQAPVQGFGATVTPAQGGYASGRNDFGR
GGGTGDARTTSTPETNPGGSGYGRR

Nucleotide


Download         Length: 1278 bp        

>NTDB_id=1102634 D3W47_RS03055 WP_119673882.1 599572..600849(-) (pilT) [Deinococcus sp. RM]
ATGACCCAGCCTGCCGCCGACATCACCGACATCCTGCGTTTCGCCGCCGACAAGGGTGCCTCCGACGTGATCATCACCGT
CGGGCTGTCCCCGCAGTTCAAGTTGCAGGGCGTGTACGACTCGCAGGGCTTCGCGGAACTCGCCCCGACCGACACCCGCA
AACTGATGTACTCCATGATGAACGAGAAGCAGCAGCGGACCTTCGAGGAACGCCGCGAACTGGACTTCTCGTTCGCGCTG
GGCGAGAAGGCCCGCTTCCGCGTGAACGCGTTCATGCAGCGCGGCAACGTGGGCGGCGTGCTGCGACTGATTCCCACGAA
GATCAAGAGCGCGCAGGAGATGGGCCTCCCGGCGAACGTCATCGAGATCGCCAACTCGCCGCGCGGCCTGGTGCTCGTGA
CCGGCCCGACCGGTTCGGGCAAGTCCACGACGCTCGCGGCGATGATCGACCACATCAACACCACCAAGCGGCTGCACATC
ATGACCATCGAGGATCCCATCGAGTTCATGCACACGCACAAGCAGTCGATCATCAACCAGCGTGAGGTCGGCGCGGACAC
CATGAGCTTCAACGACGCGCTGCGCGCCGTGCTGCGTCAGGCCCCCGACGTGATCCTCGTGGGCGAAATGCGCGACTACG
AGACAATCAAGGCCGCCGTCACCGCCGCCGAGACCGGGCACCTCGTGATGGGCACCCTGCACACGAACAGCGCGCCGGAA
TCCATCGACCGTATCGTGGACGTGTTCCCCGAGGAGCAGCAGGAGCAGATCCGCGTGCAGCTCGCGAACAACCTCGTGGC
GGTCATGACGCAGCAGCTGCTGCCGCGCCTGGACGGGCAGGGCCGCATCCTGGCGTACGAACTGCTGATCGCCAACCCGG
CCGTGCGCGCCCTGATCCGCGAGGGCAAGACGTACCAGATCACGTCCGTCATGCAGACCGGCGCGCGCGAGGGCATGGTC
ACCATGGACGCCTTCTTGGCGAACCTGTACCGCCGCCGCGTGATCTCCTTCGACACCGGCGTCGAGCGCGCCGTGGACAG
CAAGGAATTCGCCCGACTGGCCAACGACCCCAGCATCGGCACGGCGGGCGGCGCGGCCAGCATGCCCGCCGGGTACGGTC
AGGCGCCCGTGCAGGGCTTCGGCGCGACCGTCACCCCTGCCCAGGGCGGCTACGCGTCCGGTCGCAACGACTTCGGCCGG
GGCGGCGGCACCGGCGACGCCCGCACCACCAGCACCCCCGAGACCAACCCCGGCGGGAGCGGCTACGGCAGACGGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(16-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A418QGB7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

74.822

99.059

0.741
  pilT Neisseria meningitidis 8013

51.437

81.882

0.421
  pilT Neisseria gonorrhoeae MS11

51.163

80.941

0.414
  pilT Pseudomonas stutzeri DSM 10701

50.581

80.941

0.409
  pilT Pseudomonas aeruginosa PAK

50

80.941

0.405
  pilT Acinetobacter baumannii strain A118

52.121

77.647

0.405
  pilT Acinetobacter baumannii D1279779

52.121

77.647

0.405
  pilT Acinetobacter nosocomialis M2

52.121

77.647

0.405
  pilT Acinetobacter baylyi ADP1

50.737

79.765

0.405
  pilT Legionella pneumophila strain Lp02

49.128

80.941

0.398
  pilT Legionella pneumophila strain ERS1305867

49.128

80.941

0.398
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.24

77.412

0.381
  pilT Vibrio cholerae strain A1552

49.24

77.412

0.381
  pilU Acinetobacter baylyi ADP1

44.253

81.882

0.362

Multiple sequence alignment