Detailed information NTG1102635

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: D3W47_RS03060
• Genome accession: NZ_CP182821
• Coordinates: 600895..603573 (-)
• Length: 892 a.a.
• NCBI ID: WP_119673881.1
• Uniprot ID: A0A418QGF0
• Organism: Deinococcus sp. RM
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 595895..608573

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
D3W47_RS03035 (D3W47_003035)	-	596136..596627 (-)	492	WP_119673886.1	DinB family protein	-
D3W47_RS03040 (D3W47_003040)	-	596714..597703 (+)	990	WP_119673885.1	helix-turn-helix transcriptional regulator	-
D3W47_RS03045 (D3W47_003045)	-	597700..598149 (+)	450	WP_119673884.1	(4Fe-4S)-binding protein	-
D3W47_RS03050 (D3W47_003050)	-	598190..599491 (+)	1302	WP_199699837.1	thymidine phosphorylase	-
D3W47_RS03055 (D3W47_003055)	pilT	599572..600849 (-)	1278	WP_119673882.1	type IV pilus twitching motility protein PilT	Machinery gene
D3W47_RS03060 (D3W47_003060)	pilB	600895..603573 (-)	2679	WP_119673881.1	type II/IV secretion system protein	Machinery gene
D3W47_RS03065 (D3W47_003065)	-	603628..604155 (-)	528	WP_058978804.1	YqeG family HAD IIIA-type phosphatase	-
D3W47_RS03070 (D3W47_003070)	pgeF	604152..604880 (-)	729	WP_233555254.1	peptidoglycan editing factor PgeF	-
D3W47_RS03075 (D3W47_003075)	-	605017..605811 (+)	795	WP_119673879.1	enoyl-ACP reductase FabI	-
D3W47_RS03080 (D3W47_003080)	-	605865..606224 (-)	360	WP_119673878.1	winged helix-turn-helix transcriptional regulator	-
D3W47_RS03085 (D3W47_003085)	-	606403..607344 (+)	942	WP_119673877.1	NADP-dependent oxidoreductase	-
D3W47_RS03090 (D3W47_003090)	-	607424..607837 (+)	414	WP_119673876.1	isochorismatase family protein	-

Sequence

Protein

• Length: 892 a.a.
• Molecular weight: 97353.37 Da
• Isoelectric Point: 4.9834

>NTDB_id=1102635 D3W47_RS03060 WP_119673881.1 600895..603573(-) (pilB) [Deinococcus sp. RM]
MALSIGDRRLGAILLEQGYVNDTDLQKALVRHAEVGGRLAEILIDSGMVGEKRIARAIEEALGIPLVNLLVVTPEAAALQ
AVRAQTAMQYQAFPFALDGQTLRVAIVDPLSSMAIEALEDDSGLNIEVYQALRDQIMWAIASFYPELHLTVEAPAEAEGG
PAGGMLGQRLISRGLISDAQLQVALDAQQQTGEPLGSTLIAQRIISEDQLYEVLAEQSGAVFLRNPRDFQPSEDVLGSML
RADALRLTAVPVDETDHGVTVVASDPRKREDIEALVGRPVQLVLAKPRDIETLIERFYPQRGRLGEQMVQQGTLSREQLR
EALQVQAREGRVKPLGEVIVDLGFAAADEIDNALQKQNAGGGRLEDTLVQSGKLSPEMLARSLAAQLGYEFLDPVQNPPD
NKVALMIPETTARRYGVVPVRLQGESLVVAMKDPRNVFALDDLKLITGRDIVPAVMAEKDITRLIERYFGSQDMANLNQQ
LAKESKERETAGKRQDAEDLTAGLDDNAVVRVVDNIIREAALQEASDIHIEPTETALKVRYRVDGILREQNELPKGSSQS
ILARIKIMGHLDISERRVPQDGRVRFKKGSIDLDLRLSTLPTVYGEKAVMRLLQKASNIPEVEQLGFSEHNYQRYLDTIH
KPNGIFLVTGPTGSGKSFTSFSTLKRIAVPEKNTTTIEDPVEYEIPGIIQSQVNNAAGMTFARALRAFLRQDPDIIFVGE
IRDTETAKIAVEAALTGHLVLATLHTNDAPGAIVRLEEMGVEHFNIGAAVVGVVAQRLVRKVCPDCKAPTNADPDVLRRL
GITERDLRGAQLMRGAGCNRCGGTGYKGRMGIHELMVIDEPLRVAIGSGKNATEITEVAINQSGMKTLRQDGIEKALKGV
TTLEEVLAVTSK

Nucleotide

• Length: 2679 bp

>NTDB_id=1102635 D3W47_RS03060 WP_119673881.1 600895..603573(-) (pilB) [Deinococcus sp. RM]
ATGGCTCTTTCCATCGGTGACCGGCGCCTGGGCGCCATCCTGCTCGAACAGGGGTACGTGAACGACACGGACCTGCAAAA
AGCCCTGGTCCGCCACGCGGAGGTCGGCGGGCGTCTCGCGGAGATCCTGATCGACTCCGGCATGGTCGGCGAGAAACGCA
TCGCGCGCGCCATCGAGGAAGCGCTCGGCATTCCGCTCGTGAACCTGCTCGTCGTCACGCCCGAAGCGGCCGCGCTGCAG
GCGGTGCGCGCGCAGACGGCCATGCAGTACCAGGCGTTCCCGTTCGCGCTGGACGGCCAGACGCTGCGCGTGGCGATCGT
GGACCCGCTGTCGAGCATGGCCATCGAGGCGCTGGAGGACGACAGCGGCCTGAACATCGAGGTGTACCAGGCGCTGCGTG
ACCAGATCATGTGGGCGATCGCGTCGTTCTACCCCGAACTGCACCTGACGGTCGAGGCGCCCGCCGAGGCCGAGGGCGGA
CCGGCGGGCGGGATGCTGGGCCAGCGCCTGATCAGCCGCGGCCTGATCAGCGACGCGCAGCTGCAGGTGGCGCTCGACGC
GCAGCAGCAGACCGGCGAGCCGCTGGGCAGCACCCTGATTGCGCAGCGGATCATCAGCGAGGACCAGCTGTACGAGGTGC
TGGCCGAGCAGAGCGGCGCGGTGTTCCTGCGCAACCCGCGCGACTTCCAGCCCAGCGAGGACGTGCTGGGCAGCATGCTG
CGCGCCGACGCGCTGCGCCTGACCGCCGTGCCGGTCGACGAGACCGATCACGGCGTGACCGTGGTCGCCAGCGACCCGCG
CAAACGCGAGGACATCGAGGCGCTCGTCGGGCGGCCCGTGCAGCTCGTGCTGGCCAAACCGCGCGACATCGAGACCCTGA
TCGAGCGGTTCTACCCGCAGCGCGGGCGGCTGGGCGAGCAGATGGTGCAGCAGGGCACCCTGTCGCGCGAGCAGCTGCGC
GAGGCGCTGCAGGTGCAGGCCCGCGAGGGCCGCGTCAAGCCGCTGGGCGAGGTGATCGTCGACCTGGGCTTCGCCGCGGC
CGACGAGATCGACAACGCCCTGCAGAAACAGAACGCCGGGGGCGGGCGCCTGGAGGACACGCTGGTGCAGTCCGGGAAGC
TCAGCCCGGAGATGCTGGCGCGCTCGCTGGCCGCGCAGCTCGGCTACGAGTTCCTGGATCCCGTCCAGAATCCGCCGGAC
AACAAGGTCGCGCTGATGATCCCCGAGACGACCGCCCGCCGCTACGGCGTGGTCCCGGTCCGCTTGCAGGGCGAGTCGCT
GGTCGTGGCGATGAAGGACCCGCGCAACGTGTTCGCGCTGGACGACCTGAAGCTGATCACCGGGCGCGACATCGTCCCGG
CGGTCATGGCGGAAAAGGACATCACACGCCTGATCGAGCGGTACTTCGGCAGTCAGGACATGGCGAACCTGAACCAGCAG
CTCGCCAAGGAGAGCAAGGAGCGCGAGACGGCAGGCAAACGCCAGGACGCCGAGGACCTCACGGCCGGACTGGACGACAA
CGCCGTGGTGCGCGTCGTGGACAACATCATCCGCGAGGCGGCGCTGCAGGAGGCCAGCGACATCCACATCGAGCCGACCG
AGACGGCCCTGAAGGTCCGCTACCGCGTGGACGGCATCCTGCGCGAGCAGAACGAACTGCCCAAGGGCAGCTCGCAGAGC
ATCCTGGCGCGCATCAAGATCATGGGGCACCTCGACATCAGCGAGCGGCGCGTGCCGCAGGACGGCCGCGTGCGCTTCAA
GAAGGGCAGCATCGACCTGGACCTGCGACTGTCCACGCTGCCCACCGTGTACGGCGAGAAGGCCGTCATGCGTCTGCTGC
AGAAGGCCAGCAACATCCCGGAAGTCGAGCAGCTGGGCTTTTCAGAGCACAACTACCAGCGCTACCTGGACACCATCCAC
AAACCCAACGGCATCTTCCTGGTGACCGGCCCGACCGGCTCGGGGAAATCCTTCACGTCGTTCTCGACCCTCAAGCGCAT
CGCGGTGCCGGAGAAGAACACCACCACCATTGAGGACCCGGTCGAGTACGAGATTCCGGGCATCATCCAGTCGCAGGTGA
ACAACGCGGCGGGCATGACCTTCGCCCGCGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGACATCATCTTCGTGGGCGAG
ATCCGCGACACCGAGACCGCCAAGATCGCCGTGGAGGCCGCCCTGACCGGTCACCTCGTGCTGGCGACGCTGCACACGAA
CGACGCGCCGGGCGCCATCGTGCGTCTGGAGGAGATGGGCGTCGAGCACTTCAACATCGGCGCGGCCGTGGTGGGCGTGG
TCGCGCAGCGCCTGGTGCGCAAGGTCTGCCCGGACTGCAAGGCCCCCACGAACGCCGACCCGGACGTCCTGCGCCGCCTG
GGGATCACCGAACGCGACCTGCGCGGCGCGCAGCTCATGCGCGGCGCCGGCTGCAACCGCTGCGGCGGCACCGGCTACAA
GGGCCGCATGGGCATCCACGAACTGATGGTGATCGACGAACCGCTGCGCGTCGCGATCGGTTCGGGCAAGAACGCCACCG
AGATCACCGAGGTCGCCATCAACCAGAGCGGCATGAAGACCCTCCGTCAGGACGGCATCGAGAAGGCCCTCAAGGGCGTC
ACGACCCTCGAAGAGGTCCTGGCCGTCACCAGCAAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (516-781)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (43-146)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (368-473)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (199-302)

3D structure

• AlphaFold DB: A0A418QGF0

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	77.803	100	0.778
pilF	Thermus thermophilus HB27	54.738	100	0.55