Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACM1PF_RS32330 Genome accession   NZ_CP182236
Coordinates   6945817..6946932 (+) Length   371 a.a.
NCBI ID   WP_416670527.1    Uniprot ID   -
Organism   Egbenema sp. FJBBOT/2019     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6940817..6951932
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACM1PF_RS32310 (ACM1PF_32310) - 6940975..6941745 (-) 771 WP_416670522.1 PspA/IM30 family protein -
  ACM1PF_RS32315 (ACM1PF_32315) - 6941935..6942576 (+) 642 WP_416670523.1 Uma2 family endonuclease -
  ACM1PF_RS32320 (ACM1PF_32320) - 6942683..6943279 (-) 597 WP_416670524.1 hypothetical protein -
  ACM1PF_RS32325 (ACM1PF_32325) pilB/pilB1 6943689..6945695 (+) 2007 WP_416670526.1 GspE/PulE family protein Machinery gene
  ACM1PF_RS32330 (ACM1PF_32330) pilT 6945817..6946932 (+) 1116 WP_416670527.1 type IV pilus twitching motility protein PilT Machinery gene
  ACM1PF_RS32335 (ACM1PF_32335) - 6947178..6948413 (+) 1236 WP_416670528.1 type II secretion system F family protein -
  ACM1PF_RS32340 (ACM1PF_32340) - 6948455..6949078 (+) 624 WP_416670529.1 hypothetical protein -
  ACM1PF_RS32345 (ACM1PF_32345) - 6949207..6950442 (-) 1236 WP_416670531.1 esterase-like activity of phytase family protein -
  ACM1PF_RS32350 (ACM1PF_32350) - 6950550..6950738 (-) 189 WP_416670532.1 hypothetical protein -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 40772.98 Da        Isoelectric Point: 6.9679

>NTDB_id=1100958 ACM1PF_RS32330 WP_416670527.1 6945817..6946932(+) (pilT) [Egbenema sp. FJBBOT/2019]
MELMIEDLMEQLIEMGGSDMHLSAGLPPYFRINGKLTPIGDEPMTAEQCQRLIFSMLNNTQRKNLEQNWELDCSYGVKGL
ARFRVNVYKERGTYAACLRALSSKIPNFEKLGLPDVVREMSEKPRGLILVTGPTGSGKTTTLAAMIDLINRTRAEHILTV
EDPIEFVYEPIKSLVHQRQLSEDTKSFANALRAALREDPDIILVGEMRDLETISLAISAAETGHLVFGTLHTSSAAQTVD
RMIDVFPADRQTQVRVQLSNSLVAVFSQTLVSRKNPKPGEFGRVMAQEIMVVTPAISNLIREGKTAQIYSMIQTGGKQGM
QTLEKVLADLYKAGTISFEAAMSKTSKADELQRLIGGANPAAQTAKAGAAR

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=1100958 ACM1PF_RS32330 WP_416670527.1 6945817..6946932(+) (pilT) [Egbenema sp. FJBBOT/2019]
ATGGAGTTGATGATCGAAGACTTGATGGAACAACTGATCGAAATGGGTGGTTCAGATATGCACCTGTCGGCAGGACTTCC
CCCCTACTTTCGGATCAACGGTAAGCTCACCCCGATCGGCGATGAACCGATGACGGCAGAGCAGTGCCAGCGGCTGATCT
TCAGTATGTTGAACAACACCCAGCGGAAAAATCTGGAGCAGAACTGGGAATTGGACTGTTCCTATGGGGTCAAGGGCCTC
GCTCGCTTCCGGGTGAATGTTTACAAAGAGCGCGGCACCTATGCAGCCTGTTTGCGGGCTCTCAGCTCCAAGATTCCTAA
CTTTGAGAAGCTGGGCTTACCCGATGTGGTGCGGGAGATGTCTGAGAAACCCAGAGGTCTGATTCTGGTGACGGGTCCAA
CGGGGTCTGGCAAAACCACCACCCTGGCAGCCATGATTGATCTAATCAACCGCACCCGCGCGGAACACATCCTGACGGTA
GAAGACCCGATCGAATTTGTGTACGAACCGATCAAGAGTCTGGTTCACCAGCGGCAACTCAGCGAAGACACCAAGAGTTT
TGCTAACGCTCTGAGAGCCGCCCTCCGGGAAGACCCAGATATCATTCTGGTGGGTGAAATGCGAGACCTGGAAACCATCT
CCCTGGCCATTTCCGCCGCCGAAACCGGGCACCTGGTCTTTGGTACATTACACACCAGTTCCGCCGCCCAAACCGTTGAC
CGGATGATCGACGTATTTCCTGCCGATCGCCAAACCCAGGTGCGCGTTCAGCTCTCCAACTCCCTGGTCGCTGTCTTCAG
CCAAACGCTGGTGAGCCGAAAGAACCCGAAACCCGGCGAGTTTGGTCGGGTCATGGCCCAGGAAATTATGGTGGTTACGC
CCGCTATTTCCAACCTGATTCGGGAAGGAAAAACGGCCCAGATCTACTCCATGATTCAAACAGGCGGTAAGCAGGGAATG
CAGACCCTGGAAAAGGTGCTGGCTGATCTGTACAAAGCAGGAACCATCTCCTTTGAAGCGGCCATGTCCAAGACCTCCAA
AGCCGATGAACTGCAACGGCTGATCGGCGGCGCGAATCCGGCGGCTCAAACCGCTAAAGCGGGGGCAGCCAGATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(44-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

55.294

91.644

0.507
  pilT Pseudomonas stutzeri DSM 10701

53.89

93.531

0.504
  pilT Legionella pneumophila strain ERS1305867

53.333

92.992

0.496
  pilT Legionella pneumophila strain Lp02

53.333

92.992

0.496
  pilT Acinetobacter baylyi ADP1

54.303

90.836

0.493
  pilT Acinetobacter baumannii strain A118

52.819

90.836

0.48
  pilT Acinetobacter baumannii D1279779

52.819

90.836

0.48
  pilT Acinetobacter nosocomialis M2

52.819

90.836

0.48
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.411

98.922

0.469
  pilT Neisseria gonorrhoeae MS11

49.57

94.07

0.466
  pilT Neisseria meningitidis 8013

49.57

94.07

0.466
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.588

91.644

0.464
  pilT Vibrio cholerae strain A1552

50.588

91.644

0.464
  pilU Pseudomonas stutzeri DSM 10701

39.031

94.609

0.369

Multiple sequence alignment