Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   ACM1PF_RS32325 Genome accession   NZ_CP182236
Coordinates   6943689..6945695 (+) Length   668 a.a.
NCBI ID   WP_416670526.1    Uniprot ID   -
Organism   Egbenema sp. FJBBOT/2019     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6938689..6950695
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACM1PF_RS32300 (ACM1PF_32300) - 6939237..6940425 (-) 1189 Protein_6406 NAD(P)H-quinone oxidoreductase subunit H -
  ACM1PF_RS32305 (ACM1PF_32305) - 6940657..6940761 (-) 105 Protein_6407 hypothetical protein -
  ACM1PF_RS32310 (ACM1PF_32310) - 6940975..6941745 (-) 771 WP_416670522.1 PspA/IM30 family protein -
  ACM1PF_RS32315 (ACM1PF_32315) - 6941935..6942576 (+) 642 WP_416670523.1 Uma2 family endonuclease -
  ACM1PF_RS32320 (ACM1PF_32320) - 6942683..6943279 (-) 597 WP_416670524.1 hypothetical protein -
  ACM1PF_RS32325 (ACM1PF_32325) pilB/pilB1 6943689..6945695 (+) 2007 WP_416670526.1 GspE/PulE family protein Machinery gene
  ACM1PF_RS32330 (ACM1PF_32330) pilT 6945817..6946932 (+) 1116 WP_416670527.1 type IV pilus twitching motility protein PilT Machinery gene
  ACM1PF_RS32335 (ACM1PF_32335) - 6947178..6948413 (+) 1236 WP_416670528.1 type II secretion system F family protein -
  ACM1PF_RS32340 (ACM1PF_32340) - 6948455..6949078 (+) 624 WP_416670529.1 hypothetical protein -
  ACM1PF_RS32345 (ACM1PF_32345) - 6949207..6950442 (-) 1236 WP_416670531.1 esterase-like activity of phytase family protein -

Sequence

Protein


Download         Length: 668 a.a.        Molecular weight: 74341.02 Da        Isoelectric Point: 4.7875

>NTDB_id=1100957 ACM1PF_RS32325 WP_416670526.1 6943689..6945695(+) (pilB/pilB1) [Egbenema sp. FJBBOT/2019]
MANSSSQRRALVVQQNFSPFSNKLVQSGFVSGEQMQQALIESRKTGRPLTDILAMMTGQQLPPDLLRQYKKQQLFELKIL
YGVESLDPEISQINTSQMNQLIETLIPIDLCRRYRLIPLSRSESEPPFVLVAMVDPDDLAAQDDLNRVLRPQGLALQRMV
ITAEDYQRIISQYLDEQVERQKILEFQAKTDVKSDIENLEYLELEEALDDDAEANLDAALQDPEAAPVVALANKILAKAL
QDGISDIHIEPQEEHLRVRFRKDGVLRQGLDSLPKKIIPALTARFKIMANLDIAERRLPQDGRIRRLFDGRKVDFRVNTL
PSRYGEKVVLRILDNSATQLGLDKLITDPETLQIVQDMVSRPFGLILVTGPTGSGKTTTLYSALAERNDPGVNISTAEDP
IEYTLPGLTQVQVIREKGMDFASILRAFLRQDPDVILVGETRDKETAKTAIEAALTGHLVLTTLHTNDAASAVARLDEMG
IETFMVSSSLIGVLAQRLVRRVCPDCRVPYNPTPDELARFGLSASGEADVVFYKANTLSAEEANYARETDTLCPTCKGIG
YKGRSGVYEVMRVTEPIQALITEGAPTERIKEAAVEEGMKTLLAYSLNLVRQGATTLEEVERVTLTDSGVEAELKAKRKA
SLTCRCCGAALKQEWLDCPYCTTSRYQD

Nucleotide


Download         Length: 2007 bp        

>NTDB_id=1100957 ACM1PF_RS32325 WP_416670526.1 6943689..6945695(+) (pilB/pilB1) [Egbenema sp. FJBBOT/2019]
ATGGCTAACTCCTCGTCTCAACGGCGTGCCCTGGTCGTCCAACAAAACTTCTCACCGTTTAGCAACAAGCTGGTACAGTC
TGGCTTTGTTAGCGGTGAGCAGATGCAGCAAGCCCTCATCGAAAGCCGGAAAACAGGCAGGCCGCTGACTGATATTCTGG
CCATGATGACGGGGCAGCAGCTTCCGCCAGACTTGCTCCGCCAGTATAAAAAGCAGCAACTGTTTGAGCTTAAGATTCTT
TACGGTGTTGAATCGCTGGATCCGGAAATCAGCCAGATCAACACCAGCCAGATGAATCAGTTGATTGAAACCCTGATTCC
GATCGACCTTTGCCGTCGCTATCGCCTGATTCCCCTCTCCCGCAGTGAGTCAGAACCGCCCTTTGTGCTGGTGGCGATGG
TTGATCCAGACGACCTGGCAGCTCAGGACGACCTGAATCGGGTGTTGCGCCCTCAAGGGCTGGCATTGCAGCGGATGGTG
ATCACGGCTGAGGATTATCAGCGGATTATTTCCCAATATCTAGACGAGCAGGTTGAACGGCAGAAGATCCTGGAGTTTCA
GGCTAAAACCGACGTTAAAAGCGATATTGAAAACCTGGAATATCTGGAACTGGAAGAGGCGCTGGATGACGATGCCGAAG
CCAACCTGGATGCAGCTCTCCAGGATCCAGAGGCGGCTCCAGTCGTTGCCCTGGCGAACAAAATTCTGGCAAAAGCTCTC
CAGGACGGCATTTCTGATATTCACATTGAACCCCAGGAAGAACACCTGCGGGTGCGTTTTCGTAAGGACGGGGTGTTGCG
TCAGGGATTAGACTCGCTGCCGAAGAAAATTATTCCCGCCCTCACGGCCCGCTTCAAAATTATGGCCAACCTGGACATCG
CCGAGCGCCGACTGCCCCAGGACGGACGGATTCGCCGCTTATTCGATGGACGTAAGGTGGACTTCCGGGTGAATACCCTC
CCCAGCCGTTACGGGGAAAAAGTGGTTCTGCGGATTCTCGACAACTCTGCAACCCAATTAGGGCTGGACAAGCTGATCAC
CGACCCTGAAACGCTGCAAATTGTTCAGGATATGGTGTCTCGTCCCTTCGGACTGATCCTGGTGACGGGGCCAACGGGTT
CGGGTAAAACTACCACCCTTTACTCAGCCCTGGCCGAACGAAATGATCCAGGAGTCAACATCAGTACCGCTGAAGACCCG
ATCGAATATACCCTACCGGGTCTGACTCAGGTTCAGGTGATTCGGGAGAAAGGGATGGACTTTGCCTCCATTCTGCGGGC
ATTCCTGCGACAAGATCCAGATGTGATTCTGGTGGGAGAAACGCGAGACAAGGAAACCGCTAAAACGGCGATCGAAGCTG
CTCTGACCGGACACCTGGTACTGACAACCCTGCACACGAATGATGCGGCGAGTGCCGTTGCCCGCCTCGATGAAATGGGG
ATTGAAACCTTCATGGTGTCCAGTTCGCTGATTGGCGTATTGGCCCAACGACTGGTACGGCGGGTCTGTCCCGATTGCCG
GGTTCCCTACAATCCCACACCGGATGAGCTAGCCCGATTTGGACTCTCTGCCTCTGGTGAAGCCGATGTAGTCTTCTACA
AAGCCAATACCCTCAGCGCTGAGGAAGCCAATTACGCCAGAGAAACGGATACCCTCTGTCCCACCTGTAAGGGCATTGGC
TACAAGGGACGCTCCGGGGTTTATGAAGTGATGCGGGTGACAGAACCGATTCAGGCATTGATTACCGAAGGTGCGCCCAC
TGAACGCATCAAAGAGGCAGCCGTGGAAGAGGGGATGAAAACCCTTTTGGCTTACAGTCTCAATCTGGTTCGACAAGGGG
CAACCACGCTGGAGGAAGTAGAACGGGTCACATTAACCGACTCTGGGGTAGAGGCAGAACTCAAAGCCAAGCGCAAAGCT
TCCCTCACCTGCCGCTGTTGTGGGGCTGCTCTAAAGCAGGAATGGCTGGATTGTCCTTACTGCACCACATCGCGCTATCA
GGACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(234-501)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(24-178)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

65.564

99.551

0.653
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.13

92.964

0.364
  pilF Thermus thermophilus HB27

38.969

92.964

0.362

Multiple sequence alignment