Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACM66T_RS06985 Genome accession   NZ_CP182225
Coordinates   1373408..1374484 (+) Length   358 a.a.
NCBI ID   WP_231018114.1    Uniprot ID   -
Organism   Sulfurimonas sp. ST-25     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1368408..1379484
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACM66T_RS06965 (ACM66T_06965) - 1369720..1372227 (-) 2508 WP_416768196.1 sugar phosphate nucleotidyltransferase -
  ACM66T_RS06970 (ACM66T_06970) - 1372317..1372598 (-) 282 WP_231018111.1 hypothetical protein -
  ACM66T_RS06975 (ACM66T_06975) gatC 1372777..1373067 (+) 291 WP_231018112.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC -
  ACM66T_RS06980 (ACM66T_06980) - 1373064..1373411 (+) 348 WP_231018113.1 arsenate reductase family protein -
  ACM66T_RS06985 (ACM66T_06985) pilT 1373408..1374484 (+) 1077 WP_231018114.1 type IV pilus twitching motility protein PilT Machinery gene
  ACM66T_RS06990 (ACM66T_06990) - 1374578..1375927 (-) 1350 WP_231018115.1 class II 3-deoxy-7-phosphoheptulonate synthase -
  ACM66T_RS06995 (ACM66T_06995) - 1376078..1376680 (+) 603 WP_231018116.1 CvpA family protein -
  ACM66T_RS07000 (ACM66T_07000) - 1376677..1377159 (+) 483 WP_231018117.1 Fur family transcriptional regulator -
  ACM66T_RS07005 (ACM66T_07005) lysS 1377175..1378704 (+) 1530 WP_231018118.1 lysine--tRNA ligase -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 39930.93 Da        Isoelectric Point: 6.1120

>NTDB_id=1100884 ACM66T_RS06985 WP_231018114.1 1373408..1374484(+) (pilT) [Sulfurimonas sp. ST-25]
MSQTLDIRKLLKSVVAYNASDLHLVSRSEPQIRIDGRLVALNLPILDGKTIEDMSYSLLTEKQKKEFEEHNELDFAILLP
EVGRFRANYYKTMGDIACAFRIIPIEIPSLDDLNAKPIFKKIIKREKGLILVTGPTGSGKSTTLAAMLNEINETENRHII
SVEDPVEFVHQNKQCLFSHRNVGTDTANFANALKYAMREDPDVILVGEMRDKETIAAALTAAETGHLVFGTLHTNSAPQT
INRIIDVFSGDEQPQIRAQLSTSIIAIIAQALLPRLGGGRVAAQELMINIPAIANLIREDKVHQLYSQMQLNQQETGMVT
QTQELFDLVRKRVVSKQDAIQWSNRPDELIKALDAAAL

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=1100884 ACM66T_RS06985 WP_231018114.1 1373408..1374484(+) (pilT) [Sulfurimonas sp. ST-25]
ATGAGTCAGACCCTGGATATCCGTAAACTGTTAAAGAGCGTCGTTGCCTACAATGCATCGGACCTTCACCTCGTTTCCCG
TTCCGAACCGCAGATCCGTATCGACGGGCGCCTTGTCGCACTGAACCTGCCCATCCTCGACGGCAAAACAATTGAGGACA
TGTCCTACTCCCTGCTGACCGAGAAACAGAAGAAAGAGTTCGAAGAGCATAACGAACTCGACTTCGCGATCCTGCTCCCC
GAAGTCGGCCGTTTCCGTGCGAACTACTATAAGACGATGGGCGATATCGCCTGCGCCTTCCGTATCATTCCGATCGAGAT
CCCGAGCCTGGACGATCTCAACGCCAAGCCTATCTTCAAAAAAATCATTAAACGCGAAAAGGGGCTGATCCTCGTCACCG
GCCCGACCGGTAGCGGTAAGTCCACCACCCTGGCCGCGATGCTCAACGAGATCAACGAAACGGAAAACCGCCACATTATC
AGTGTCGAGGACCCGGTCGAATTCGTCCACCAGAACAAACAGTGTCTCTTCTCCCACCGTAACGTCGGGACCGATACCGC
CAACTTCGCCAACGCGCTCAAATACGCGATGCGTGAGGACCCCGATGTCATCCTTGTCGGGGAGATGCGTGACAAGGAGA
CCATCGCCGCGGCGCTCACCGCCGCCGAGACCGGTCACCTCGTTTTCGGTACCCTGCACACCAACTCCGCGCCGCAGACG
ATCAACCGTATCATCGACGTTTTCAGCGGGGACGAGCAGCCGCAGATCCGCGCCCAGCTCTCCACGTCCATCATTGCCAT
TATCGCCCAGGCGCTGCTGCCGCGCCTCGGCGGCGGCCGTGTCGCTGCCCAGGAGCTGATGATCAACATCCCGGCGATCG
CCAACCTGATCCGCGAAGACAAGGTCCACCAGCTCTACTCGCAGATGCAGCTCAACCAGCAGGAGACCGGCATGGTCACC
CAGACACAGGAGCTCTTCGACCTGGTGCGCAAACGCGTCGTCTCCAAGCAGGACGCTATCCAGTGGTCCAACCGCCCCGA
CGAACTTATCAAGGCGCTCGACGCCGCCGCGCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

51.734

96.648

0.5
  pilT Acinetobacter baumannii D1279779

51.734

96.648

0.5
  pilT Acinetobacter baumannii strain A118

51.734

96.648

0.5
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

53.293

93.296

0.497
  pilT Vibrio cholerae strain A1552

53.293

93.296

0.497
  pilT Legionella pneumophila strain ERS1305867

52.239

93.575

0.489
  pilT Legionella pneumophila strain Lp02

52.239

93.575

0.489
  pilT Pseudomonas stutzeri DSM 10701

50.145

96.369

0.483
  pilT Acinetobacter baylyi ADP1

49.855

96.369

0.48
  pilT Pseudomonas aeruginosa PAK

48.986

96.369

0.472
  pilT Neisseria gonorrhoeae MS11

49.702

93.855

0.466
  pilT Neisseria meningitidis 8013

49.405

93.855

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.011

100

0.441
  pilU Acinetobacter baylyi ADP1

40.058

96.927

0.388
  pilU Pseudomonas stutzeri DSM 10701

37.861

96.648

0.366

Multiple sequence alignment