Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   ACGHM3_RS07215 Genome accession   NZ_AP031496
Coordinates   1662343..1662798 (+) Length   151 a.a.
NCBI ID   WP_345417456.1    Uniprot ID   A0AAV3TYF1
Organism   Halioxenophilus aromaticivorans strain KU68F     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1657343..1667798
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACGHM3_RS07200 (R50071_14490) pilD 1658035..1658898 (-) 864 WP_345417447.1 A24 family peptidase Machinery gene
  ACGHM3_RS07205 (R50071_14500) pilC 1658919..1660139 (-) 1221 WP_345417450.1 type II secretion system F family protein Machinery gene
  ACGHM3_RS07210 (R50071_14510) pilB 1660145..1661869 (-) 1725 WP_345417453.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACGHM3_RS07215 (R50071_14520) pilE 1662343..1662798 (+) 456 WP_345417456.1 pilin Machinery gene
  ACGHM3_RS07220 - 1662963..1664870 (+) 1908 WP_345417459.1 hypothetical protein -
  ACGHM3_RS07225 (R50071_14540) - 1664863..1665720 (+) 858 WP_345417462.1 glycosyltransferase family 2 protein -
  ACGHM3_RS07230 (R50071_14550) - 1665730..1666569 (+) 840 WP_345417465.1 glycosyltransferase -
  ACGHM3_RS07235 (R50071_14560) - 1666562..1667749 (+) 1188 WP_345417468.1 glycosyltransferase -

Sequence

Protein


Download         Length: 151 a.a.        Molecular weight: 15621.62 Da        Isoelectric Point: 4.0373

>NTDB_id=109879 ACGHM3_RS07215 WP_345417456.1 1662343..1662798(+) (pilE) [Halioxenophilus aromaticivorans strain KU68F]
MKQQAQAGFTLIELMIVVAIIGILAAVALPAYQDYTARSQVSEAVTLASGLKATVSEIYATEGTFDEADSEDKGLPAAAD
VKGNYVQEVEVTNGVIVALMASSGIGEQIQDKTLTLSPSTTAGSITWVCGGASKDSTNGIADKYLPAECRS

Nucleotide


Download         Length: 456 bp        

>NTDB_id=109879 ACGHM3_RS07215 WP_345417456.1 1662343..1662798(+) (pilE) [Halioxenophilus aromaticivorans strain KU68F]
ATGAAACAACAAGCACAAGCAGGTTTTACCCTAATCGAATTGATGATCGTTGTTGCGATTATCGGTATTTTGGCAGCAGT
TGCACTGCCCGCCTACCAAGACTACACCGCCCGTTCTCAGGTTTCTGAGGCAGTAACCTTGGCTTCTGGTTTGAAGGCCA
CTGTGTCTGAGATATACGCAACTGAAGGGACGTTCGATGAAGCTGATAGTGAAGACAAAGGTTTGCCTGCAGCCGCTGAT
GTGAAAGGTAACTATGTCCAAGAGGTTGAGGTTACAAATGGCGTCATCGTTGCGCTCATGGCCAGCTCTGGCATTGGCGA
GCAGATTCAAGATAAAACTCTTACCTTGAGCCCTTCAACTACCGCTGGCTCAATTACCTGGGTTTGTGGTGGCGCGTCGA
AAGATTCCACAAATGGCATTGCTGACAAGTACCTGCCTGCTGAATGTCGTAGCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(5-29)

Pilin (bacterial filament) (Pilin)

(37-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae strain FA1090

51.613

100

0.53
  pilE Neisseria gonorrhoeae MS11

49.677

100

0.51
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

37.5

100

0.477
  comP Acinetobacter baylyi ADP1

43.038

100

0.45
  pilA2 Legionella pneumophila strain ERS1305867

43.537

97.351

0.424
  pilA2 Legionella pneumophila str. Paris

42.568

98.013

0.417
  pilA Ralstonia pseudosolanacearum GMI1000

37.278

100

0.417
  pilA/pilA1 Eikenella corrodens VA1

39.13

100

0.417
  pilA/pilAI Pseudomonas stutzeri DSM 10701

36.478

100

0.384
  pilA Vibrio campbellii strain DS40M4

37.255

100

0.377
  pilA Pseudomonas aeruginosa PAK

36.538

100

0.377

Multiple sequence alignment