Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACGHM3_RS07210 Genome accession   NZ_AP031496
Coordinates   1660145..1661869 (-) Length   574 a.a.
NCBI ID   WP_345417453.1    Uniprot ID   A0AAV3TYR7
Organism   Halioxenophilus aromaticivorans strain KU68F     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1655145..1666869
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACGHM3_RS07170 (R50071_14430) mutT 1655427..1655837 (+) 411 WP_345417429.1 8-oxo-dGTP diphosphatase MutT -
  ACGHM3_RS07175 (R50071_14440) - 1655911..1656171 (-) 261 WP_345417432.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -
  ACGHM3_RS07180 (R50071_14450) yacG 1656304..1656516 (-) 213 WP_345417435.1 DNA gyrase inhibitor YacG -
  ACGHM3_RS07185 (R50071_14460) - 1656577..1656861 (-) 285 WP_345417438.1 addiction module antidote protein -
  ACGHM3_RS07190 (R50071_14470) - 1656864..1657160 (-) 297 WP_345417441.1 type II toxin-antitoxin system RelE/ParE family toxin -
  ACGHM3_RS07195 (R50071_14480) coaE 1657216..1657818 (-) 603 WP_345417444.1 dephospho-CoA kinase -
  ACGHM3_RS07200 (R50071_14490) pilD 1658035..1658898 (-) 864 WP_345417447.1 A24 family peptidase Machinery gene
  ACGHM3_RS07205 (R50071_14500) pilC 1658919..1660139 (-) 1221 WP_345417450.1 type II secretion system F family protein Machinery gene
  ACGHM3_RS07210 (R50071_14510) pilB 1660145..1661869 (-) 1725 WP_345417453.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACGHM3_RS07215 (R50071_14520) pilE 1662343..1662798 (+) 456 WP_345417456.1 pilin Machinery gene
  ACGHM3_RS07220 - 1662963..1664870 (+) 1908 WP_345417459.1 hypothetical protein -
  ACGHM3_RS07225 (R50071_14540) - 1664863..1665720 (+) 858 WP_345417462.1 glycosyltransferase family 2 protein -
  ACGHM3_RS07230 (R50071_14550) - 1665730..1666569 (+) 840 WP_345417465.1 glycosyltransferase -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 62826.60 Da        Isoelectric Point: 4.7558

>NTDB_id=109878 ACGHM3_RS07210 WP_345417453.1 1660145..1661869(-) (pilB) [Halioxenophilus aromaticivorans strain KU68F]
MAQQEINLSGLARRLVNDGLLSEDDAKNALVKAKAERIPFAWYLTNANLVAAAKIAQAAGDEFGTPVFDLSTLRPEAMPR
KLVDAKLLHKHHALPLFKRGNRLFIGIADPTNLQAIDEIKFNTGLSTDAVLVESDKLQKALEAYLNSEDESLGDSLGGLD
DDSLEDLDVEAFDPDAQDDDDSDVDEAPIVRFVNKVLLDAIKSGASDIHFEPYEKTYRVRFRRDGILTEVAKPPSSLATR
ISARLKVMSQMDISERRIPQDGRIKMKLSKTRAIDFRVNSLPTLFGEKIVLRILDPSSAKLGIDALGYEEDQKKIFMDAL
AQPQGMILVTGPTGSGKTVSLYTGLNILNTEECNISTAEDPVEINLEGINQVNMNSKVGLTFAEALRSFLRQDPDVVMVG
EIRDLETAEIAIKAAQTGHLVLSTLHTNSAPETLTRLLNMGVPAFNVATSVSVIIAQRLARRLCPSCKKPAEDIPDEILT
EEGFDNIGIPREELALYQPVGCDACSKGYKGRVGVYEVVRITDSISRIIMEGGNSIQIADQARKEGFNNLRISALRKAAI
GVTSLEEANRVTKD

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=109878 ACGHM3_RS07210 WP_345417453.1 1660145..1661869(-) (pilB) [Halioxenophilus aromaticivorans strain KU68F]
ATGGCACAACAGGAAATTAATCTAAGCGGCTTAGCACGCCGGCTGGTAAACGATGGCTTACTTTCAGAGGATGACGCCAA
AAACGCTTTGGTTAAGGCTAAGGCGGAGCGCATTCCCTTTGCTTGGTACCTCACCAACGCCAACCTGGTTGCGGCGGCTA
AAATTGCCCAGGCCGCCGGCGACGAATTTGGCACCCCGGTGTTTGATTTAAGCACCCTGCGGCCAGAGGCCATGCCGCGC
AAGCTGGTAGACGCCAAATTATTGCACAAACACCATGCCTTACCCTTGTTTAAACGCGGCAACCGACTGTTTATTGGCAT
CGCGGACCCTACCAACCTGCAAGCCATCGATGAAATTAAATTTAACACCGGCCTTAGTACCGATGCGGTACTGGTAGAAA
GTGACAAACTGCAAAAAGCGTTAGAGGCCTACCTTAATAGTGAAGATGAATCCCTAGGTGATTCCCTAGGCGGGTTGGAT
GACGACTCTTTAGAAGACCTAGACGTAGAAGCCTTTGACCCAGATGCCCAGGATGATGACGACTCCGACGTAGACGAAGC
CCCCATCGTAAGGTTTGTGAACAAAGTATTGTTGGATGCCATTAAATCTGGCGCCTCGGATATTCACTTTGAGCCCTACG
AGAAGACCTACCGTGTGCGTTTTCGCCGGGATGGCATATTAACCGAGGTGGCCAAGCCGCCCTCCTCGCTGGCCACCCGA
ATCTCCGCCCGGTTAAAGGTGATGTCGCAAATGGATATCTCCGAACGGCGTATACCCCAGGATGGCCGCATTAAAATGAA
GTTGTCCAAAACCCGGGCCATTGATTTTCGGGTCAACTCATTGCCAACGTTGTTTGGCGAAAAGATAGTATTGCGGATAT
TGGACCCAAGCAGTGCCAAATTGGGTATTGATGCCCTAGGCTATGAGGAAGACCAGAAAAAGATCTTTATGGATGCCCTG
GCCCAGCCCCAGGGTATGATTTTGGTAACCGGGCCTACCGGCTCGGGTAAAACGGTATCGCTCTATACGGGGCTGAATAT
CCTAAATACGGAAGAATGTAATATCTCCACAGCGGAAGACCCGGTAGAGATTAACCTGGAAGGCATTAACCAGGTCAATA
TGAACTCTAAAGTGGGCCTAACCTTTGCCGAGGCACTGCGATCCTTCTTACGGCAAGACCCAGACGTGGTGATGGTGGGC
GAGATTCGAGATCTGGAAACCGCCGAAATCGCCATTAAAGCGGCCCAAACCGGCCACTTGGTGCTGTCTACCCTCCACAC
CAACAGCGCCCCAGAAACCCTCACCCGGCTGTTAAATATGGGCGTACCAGCGTTTAACGTGGCCACCTCGGTAAGCGTGA
TTATTGCCCAGCGGCTGGCCCGGCGCTTATGCCCTAGCTGTAAAAAACCGGCCGAGGATATACCGGATGAAATTTTAACC
GAAGAAGGCTTTGATAATATTGGCATCCCCCGGGAAGAGTTGGCGCTATACCAACCCGTTGGCTGCGATGCCTGCAGTAA
AGGTTATAAGGGGCGAGTGGGTGTGTACGAAGTGGTTCGCATCACCGATAGTATTTCTCGCATTATAATGGAAGGTGGTA
ACTCCATTCAGATTGCAGACCAAGCCCGCAAAGAGGGCTTTAACAATCTGCGAATTTCTGCGCTGCGTAAGGCGGCTATA
GGCGTTACCAGCCTCGAAGAGGCTAACCGAGTCACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(195-462)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

60.808

99.129

0.603
  pilB Acinetobacter baylyi ADP1

59.298

99.303

0.589
  pilB Legionella pneumophila strain ERS1305867

54.957

100

0.551
  pilB Vibrio parahaemolyticus RIMD 2210633

50.704

98.955

0.502
  pilB Vibrio campbellii strain DS40M4

49.823

98.606

0.491
  pilB Vibrio cholerae strain A1552

50.817

95.993

0.488
  pilF Neisseria gonorrhoeae MS11

47.1

99.129

0.467
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.333

100

0.38
  pilB/pilB1 Synechocystis sp. PCC 6803

41.235

87.456

0.361

Multiple sequence alignment