Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACMXUA_RS01795 Genome accession   NZ_CP181076
Coordinates   382125..383105 (+) Length   326 a.a.
NCBI ID   WP_032676501.1    Uniprot ID   A0A9Q2WBJ7
Organism   Enterobacter hormaechei strain CYEBC080     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 377125..388105
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMXUA_RS01755 (ACMXUA_01755) - 377200..377526 (+) 327 WP_003862421.1 YggL family protein -
  ACMXUA_RS01760 (ACMXUA_01760) - 377584..378300 (+) 717 WP_022649360.1 DUF2884 domain-containing protein -
  ACMXUA_RS01765 (ACMXUA_01765) - 378368..378730 (-) 363 WP_023306916.1 endonuclease domain-containing protein -
  ACMXUA_RS01770 (ACMXUA_01770) hemW 378787..379935 (-) 1149 WP_023306915.1 radical SAM family heme chaperone HemW -
  ACMXUA_RS01775 (ACMXUA_01775) - 379928..380521 (-) 594 WP_022649357.1 XTP/dITP diphosphatase -
  ACMXUA_RS01780 (ACMXUA_01780) yggU 380525..380821 (-) 297 WP_022649356.1 DUF167 family protein YggU -
  ACMXUA_RS01785 (ACMXUA_01785) - 380818..381384 (-) 567 WP_003860024.1 YggT family protein -
  ACMXUA_RS01790 (ACMXUA_01790) - 381406..382107 (-) 702 WP_023298569.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACMXUA_RS01795 (ACMXUA_01795) pilT 382125..383105 (+) 981 WP_032676501.1 type IV pilus twitching motility protein PilT Machinery gene
  ACMXUA_RS01800 (ACMXUA_01800) ruvX 383116..383532 (-) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  ACMXUA_RS01805 (ACMXUA_01805) - 383532..384092 (-) 561 WP_022649353.1 YqgE/AlgH family protein -
  ACMXUA_RS01810 (ACMXUA_01810) gshB 384167..385114 (-) 948 WP_023306913.1 glutathione synthase -
  ACMXUA_RS01815 (ACMXUA_01815) rsmE 385146..385877 (-) 732 WP_003860033.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACMXUA_RS01820 (ACMXUA_01820) endA 385929..386636 (-) 708 WP_023306912.1 deoxyribonuclease I -
  ACMXUA_RS01825 (ACMXUA_01825) - 386731..387228 (-) 498 WP_032621896.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35673.66 Da        Isoelectric Point: 6.6603

>NTDB_id=1098012 ACMXUA_RS01795 WP_032676501.1 382125..383105(+) (pilT) [Enterobacter hormaechei strain CYEBC080]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVEALLKAWLNDEQQGAWWANGQVDFAVTLTGGQRLRG
SAFKHMHGVSIALRLLPLTCPQLSALGVPRAIPELLSNDNGLILVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
FMYQSQRCLIRQREIGQHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLRQDVQGGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=1098012 ACMXUA_RS01795 WP_032676501.1 382125..383105(+) (pilT) [Enterobacter hormaechei strain CYEBC080]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCACCACCGCG
CTGGCGCAGGTCAGGCCGTCTTGAACCTGCGCCGTTTCCGCCCCCGGATGTGGAGGCGTTATTAAAAGCGTGGCTCAATG
ACGAACAGCAGGGCGCCTGGTGGGCAAACGGGCAGGTAGATTTTGCCGTTACCCTCACAGGCGGTCAGCGGCTGCGCGGC
AGTGCGTTTAAGCATATGCACGGCGTTTCGATCGCGCTACGCCTGTTGCCGCTGACGTGTCCGCAGCTCTCTGCGCTAGG
CGTGCCGCGCGCGATCCCGGAGCTTTTGTCCAATGACAATGGCCTGATTCTGGTCACCGGCGCCACCGGCAGCGGGAAAT
CGACCACCCTGGCCGCGATGGTCGATTTTCTCAATCACCATACGGACGGGCACATTCTGACCCTGGAAGATCCGGTGGAG
TTTATGTACCAGAGCCAACGTTGCCTCATCCGGCAGCGGGAGATAGGCCAGCATAGCCCGTCCTTTGCCGAGGCCCTGCG
CAGTGCGCTGCGTGAAGATCCGGATGTGATTCTGCTTGGTGAGCTGCGCGACAGCGAGACGATACGCCTGGCGCTGACGG
CGGCTGAGACTGGCCATCTGGTGTTGGCCACGCTGCACACGCGCGGTGCGGCGCAGGCGATCGAGCGCCTGGTCGATACC
TTCCCGGCGCAGGAGAAAGATCCGGTTCGTAATCAGCTGGCGGGAAGCCTGCGTGCGGTACTGGCGCAGAAGCTGCGTCA
GGATGTCCAGGGCGGGCGCGTGGCGCTGTATGAGCTACTGGTGAATACGTCGGCGGCGGCAAACCTGATCCGCGAAGGTA
AAACCTGGCAACTGCCGGGCATTATTCAAACGGGGCAGCAGGCGGGGATGCAGAACTTTGAGCAGAGTCTGGCGGAGCGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(75-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.765

100

0.509
  pilT Vibrio cholerae strain A1552

50.765

100

0.509
  pilT Pseudomonas stutzeri DSM 10701

48.012

100

0.482
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Legionella pneumophila strain ERS1305867

48.447

98.773

0.479
  pilT Legionella pneumophila strain Lp02

48.447

98.773

0.479
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Acinetobacter baumannii strain A118

47.095

100

0.472
  pilT Acinetobacter baylyi ADP1

47.095

100

0.472
  pilT Acinetobacter baumannii D1279779

47.095

100

0.472
  pilT Pseudomonas aeruginosa PAK

47.095

100

0.472
  pilT Acinetobacter nosocomialis M2

47.5

98.16

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.344

99.08

0.429
  pilU Pseudomonas stutzeri DSM 10701

38.485

100

0.39
  pilU Vibrio cholerae strain A1552

38.509

98.773

0.38

Multiple sequence alignment