Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACLM44_RS08900 Genome accession   NZ_CP180405
Coordinates   1664314..1665387 (+) Length   357 a.a.
NCBI ID   WP_006042669.1    Uniprot ID   -
Organism   Synechococcus sp. W2B2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1659314..1670387
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLM44_RS08875 (ACLM44_08875) rsgA 1659509..1660390 (-) 882 WP_038005295.1 ribosome small subunit-dependent GTPase A -
  ACLM44_RS08880 (ACLM44_08880) - 1660410..1660652 (-) 243 WP_006042665.1 sulfurtransferase TusA family protein -
  ACLM44_RS08885 (ACLM44_08885) dnaJ 1660655..1661785 (-) 1131 WP_006042666.1 molecular chaperone DnaJ -
  ACLM44_RS08890 (ACLM44_08890) grpE 1661836..1662549 (-) 714 WP_006042667.1 nucleotide exchange factor GrpE -
  ACLM44_RS08895 (ACLM44_08895) pilB/pilB1 1662645..1664303 (+) 1659 WP_038004581.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  ACLM44_RS08900 (ACLM44_08900) pilT 1664314..1665387 (+) 1074 WP_006042669.1 type IV pilus twitching motility protein PilT Machinery gene
  ACLM44_RS08905 (ACLM44_08905) - 1665404..1666657 (+) 1254 WP_006042670.1 type II secretion system F family protein -
  ACLM44_RS08910 (ACLM44_08910) - 1666692..1667954 (-) 1263 WP_006042671.1 NAD(P)/FAD-dependent oxidoreductase -
  ACLM44_RS08915 (ACLM44_08915) msrB 1667926..1668444 (-) 519 WP_006042672.1 peptide-methionine (R)-S-oxide reductase MsrB -
  ACLM44_RS08920 (ACLM44_08920) dusA 1668506..1669510 (+) 1005 WP_006042673.1 tRNA dihydrouridine(20/20a) synthase DusA -
  ACLM44_RS08925 (ACLM44_08925) - 1669529..1670122 (-) 594 WP_006042674.1 RNA recognition motif domain-containing protein -

Sequence

Protein


Download         Length: 357 a.a.        Molecular weight: 39413.13 Da        Isoelectric Point: 6.6100

>NTDB_id=1095711 ACLM44_RS08900 WP_006042669.1 1664314..1665387(+) (pilT) [Synechococcus sp. W2B2]
MELMIEDLMQELVEAGGSDLHIASGQPPYGRFSGQLRPMRDEPLMEESCNRLIFSMLNNSQRKTLEQTWELDCAYGLKGV
ARFRVNVYRQKGSYAACLRALGSKIPSIELLNLPPVVVETSKRPRGLVLVTGPTWSGKTTTLAALLDHINHSRAEHILTI
EDPIEFVYKSDQSLVHQRQLNEDTRSFANALRAALREDPDVILVGEMRDLETIQLAISAAETGHLVFGTLHTSSAAQTVD
RMVDVFPPAQQTQIRVQLSGSLVAVFSQTLCRRRNPAPGQFGRVMAQEIMINTPAIANLIREGKTAQLYSQIQTGGELGM
QTLEKALADLINRGDITLQEARAKASKPSELDRLAAG

Nucleotide


Download         Length: 1074 bp        

>NTDB_id=1095711 ACLM44_RS08900 WP_006042669.1 1664314..1665387(+) (pilT) [Synechococcus sp. W2B2]
ATGGAACTGATGATCGAGGACCTGATGCAGGAGCTGGTGGAGGCAGGCGGCAGCGACCTTCACATCGCCAGCGGTCAACC
CCCCTACGGCCGCTTCAGCGGCCAGTTGCGGCCCATGCGCGACGAGCCCCTGATGGAGGAAAGCTGCAACCGGCTGATCT
TCTCCATGCTTAACAACAGCCAGCGCAAAACACTCGAACAGACCTGGGAGCTCGACTGTGCCTACGGACTCAAAGGCGTG
GCCCGCTTCCGGGTGAATGTATACCGCCAGAAAGGCAGCTATGCCGCCTGTCTGCGCGCTCTCGGTAGCAAAATCCCCAG
CATCGAACTGCTGAATCTTCCTCCCGTGGTGGTGGAAACGAGCAAACGCCCCCGGGGATTAGTGCTCGTGACCGGCCCGA
CTTGGTCAGGCAAAACCACCACCCTGGCGGCCCTGCTGGATCACATCAACCACAGCCGGGCGGAACACATCCTCACGATC
GAGGATCCGATCGAATTCGTGTACAAGAGCGATCAGAGCCTGGTGCATCAACGCCAGCTCAACGAAGACACCCGCAGTTT
CGCCAATGCACTGCGAGCAGCCCTGAGGGAAGACCCCGACGTCATCCTGGTGGGAGAAATGCGCGATCTGGAAACCATCC
AGCTGGCGATCAGCGCTGCTGAAACCGGACACCTGGTGTTCGGCACGTTGCACACCAGCTCAGCGGCCCAGACCGTGGAC
CGCATGGTGGATGTGTTCCCTCCCGCTCAGCAGACCCAAATCCGCGTGCAGCTCTCGGGGAGCCTGGTAGCCGTGTTCTC
TCAAACCCTATGCCGCCGCCGCAACCCTGCACCTGGACAATTCGGCCGGGTGATGGCGCAGGAAATCATGATCAACACGC
CGGCCATCGCCAATCTCATCCGCGAAGGAAAAACCGCCCAGCTGTATTCACAGATTCAAACCGGTGGCGAACTGGGCATG
CAGACCCTGGAGAAGGCGCTAGCAGATCTGATCAATCGAGGAGACATCACCCTTCAGGAGGCGCGGGCAAAGGCGAGCAA
ACCCTCCGAACTGGATCGGCTCGCGGCTGGTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(121-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

53.026

97.199

0.515
  pilT Pseudomonas stutzeri DSM 10701

52.45

97.199

0.51
  pilT Legionella pneumophila strain Lp02

51.594

96.639

0.499
  pilT Legionella pneumophila strain ERS1305867

51.594

96.639

0.499
  pilT Acinetobacter baylyi ADP1

51.149

97.479

0.499
  pilT Acinetobacter nosocomialis M2

51.009

97.199

0.496
  pilT Acinetobacter baumannii D1279779

51.009

97.199

0.496
  pilT Acinetobacter baumannii strain A118

51.009

97.199

0.496
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.573

98.319

0.487
  pilT Neisseria meningitidis 8013

49.563

96.078

0.476
  pilT Vibrio cholerae strain A1552

50

95.238

0.476
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50

95.238

0.476
  pilT Neisseria gonorrhoeae MS11

49.271

96.078

0.473
  pilU Pseudomonas stutzeri DSM 10701

38.905

97.199

0.378

Multiple sequence alignment