Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   ACLM44_RS08895 Genome accession   NZ_CP180405
Coordinates   1662645..1664303 (+) Length   552 a.a.
NCBI ID   WP_038004581.1    Uniprot ID   -
Organism   Synechococcus sp. W2B2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1657645..1669303
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLM44_RS08865 (ACLM44_08865) murB 1658236..1659168 (+) 933 WP_006042662.1 UDP-N-acetylmuramate dehydrogenase -
  ACLM44_RS08870 (ACLM44_08870) - 1659193..1659534 (+) 342 WP_006042663.1 YbaB/EbfC family nucleoid-associated protein -
  ACLM44_RS08875 (ACLM44_08875) rsgA 1659509..1660390 (-) 882 WP_038005295.1 ribosome small subunit-dependent GTPase A -
  ACLM44_RS08880 (ACLM44_08880) - 1660410..1660652 (-) 243 WP_006042665.1 sulfurtransferase TusA family protein -
  ACLM44_RS08885 (ACLM44_08885) dnaJ 1660655..1661785 (-) 1131 WP_006042666.1 molecular chaperone DnaJ -
  ACLM44_RS08890 (ACLM44_08890) grpE 1661836..1662549 (-) 714 WP_006042667.1 nucleotide exchange factor GrpE -
  ACLM44_RS08895 (ACLM44_08895) pilB/pilB1 1662645..1664303 (+) 1659 WP_038004581.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  ACLM44_RS08900 (ACLM44_08900) pilT 1664314..1665387 (+) 1074 WP_006042669.1 type IV pilus twitching motility protein PilT Machinery gene
  ACLM44_RS08905 (ACLM44_08905) - 1665404..1666657 (+) 1254 WP_006042670.1 type II secretion system F family protein -
  ACLM44_RS08910 (ACLM44_08910) - 1666692..1667954 (-) 1263 WP_006042671.1 NAD(P)/FAD-dependent oxidoreductase -
  ACLM44_RS08915 (ACLM44_08915) msrB 1667926..1668444 (-) 519 WP_006042672.1 peptide-methionine (R)-S-oxide reductase MsrB -

Sequence

Protein


Download         Length: 552 a.a.        Molecular weight: 60233.47 Da        Isoelectric Point: 4.7503

>NTDB_id=1095710 ACLM44_RS08895 WP_038004581.1 1662645..1664303(+) (pilB/pilB1) [Synechococcus sp. W2B2]
MSDVRRRLELELLLKRAIPGTLSSADEPDLLNDLAAMGLDPTRHGELTAALSSLLKPARAEPGQAPDDNDENTESIADTA
TSYLAEFTVDGVLEDDPDQAALQSSSIVDLEDGLNDADRSPVINLVDRILMQALQLGASDIHVEPQQAGLQLRFRQDGVL
QSSIEPLPSRLVPAVTSRFKIMADLDIAERRMAQDGRIRRRFQHRTVDFRVNCLPSRFGEKIVLRLLDSSATQLGLDKLI
TSESTLDTVRSLGSKPFGMILVTGPTGSGKSTTLYSLLAERNDPGINISTVEDPIEYTLPGITQCQVNREKGFDFSQALR
AFMRQDPDVLLVGETRDLETAKTAIEAALTGHLVLTTLHCNDAPSAIARLDEMGVEPFMVSASLIGIVSQRLLRRVCPDC
RIPYHPKAEELGRFGLMTSNEEGVTFFKPNHREGHPDACPTCQGSGYKGRVGVYEVLRMNEVMAAAVAKGATTDMVRQLA
LESGMKTLLGYSLDLVREGRTTLEEVGRMVLTDAGLESERRARALSTLSCSGCGGGLQEGWLECPYCLTPRQ

Nucleotide


Download         Length: 1659 bp        

>NTDB_id=1095710 ACLM44_RS08895 WP_038004581.1 1662645..1664303(+) (pilB/pilB1) [Synechococcus sp. W2B2]
GTGAGTGACGTTCGCCGACGTTTGGAACTGGAACTGCTGCTGAAGCGAGCGATCCCTGGGACTCTCAGCAGCGCTGACGA
ACCGGATCTTCTGAACGATCTCGCGGCGATGGGACTGGATCCCACTCGCCATGGCGAACTGACTGCGGCGCTTAGCAGCC
TTCTCAAGCCAGCAAGGGCGGAACCCGGTCAGGCCCCAGACGACAACGACGAAAACACGGAGAGCATCGCCGACACGGCC
ACCTCCTATCTCGCGGAATTCACCGTGGACGGGGTACTGGAAGACGACCCCGATCAAGCCGCGCTGCAGTCTTCCTCCAT
CGTGGATCTGGAAGACGGACTGAACGATGCGGATCGTTCACCGGTGATCAATCTCGTGGACCGGATCCTGATGCAGGCGC
TGCAGCTGGGAGCCAGCGATATTCACGTGGAGCCCCAGCAGGCTGGGCTGCAATTGCGCTTCCGCCAGGACGGCGTTCTG
CAGAGCAGCATCGAACCTCTGCCGAGCCGCTTGGTACCGGCGGTGACCTCCCGCTTCAAAATCATGGCGGACTTAGATAT
TGCTGAACGGCGCATGGCCCAGGACGGGCGAATCCGGCGCAGATTCCAGCACCGCACCGTGGACTTCAGGGTGAATTGCC
TGCCCAGCCGATTTGGGGAAAAGATCGTGTTACGCCTGCTGGATAGCTCCGCCACTCAGCTAGGCCTCGACAAGCTGATC
ACGAGTGAGTCAACGCTTGACACCGTGCGATCCCTGGGTTCCAAACCCTTCGGAATGATCCTGGTGACCGGGCCCACCGG
ATCGGGGAAATCCACCACGCTCTATTCCCTGCTGGCGGAACGCAACGACCCCGGGATCAATATCTCCACAGTGGAGGATC
CGATCGAATACACCCTCCCAGGCATAACCCAATGCCAAGTGAACCGGGAGAAAGGTTTCGATTTCAGCCAGGCGCTGCGG
GCCTTCATGCGCCAGGACCCGGATGTGCTGCTCGTGGGGGAGACCCGTGACTTGGAAACAGCCAAGACGGCCATCGAAGC
AGCACTGACAGGTCACCTCGTGCTCACCACGTTGCATTGCAACGACGCTCCCAGTGCCATCGCCCGACTGGATGAGATGG
GCGTTGAACCCTTCATGGTGAGCGCGTCACTGATCGGCATCGTGTCCCAGCGATTGCTGCGGCGTGTCTGCCCGGACTGC
CGCATTCCTTACCACCCGAAAGCTGAGGAACTTGGTCGGTTCGGTTTGATGACCAGCAATGAGGAGGGCGTGACGTTCTT
CAAACCCAACCACCGAGAAGGGCATCCGGACGCATGTCCCACCTGTCAGGGGAGCGGCTACAAGGGCCGGGTGGGGGTTT
ATGAGGTGCTGCGCATGAATGAAGTGATGGCTGCTGCGGTGGCCAAGGGAGCCACCACCGACATGGTGCGCCAGCTGGCC
CTGGAATCAGGGATGAAAACACTGTTGGGCTACAGCCTTGATCTGGTTCGCGAAGGGCGCACCACCCTGGAGGAAGTCGG
CCGCATGGTGCTCACTGATGCAGGCCTCGAATCCGAACGCCGGGCCCGAGCCCTGAGCACGCTCAGCTGCAGCGGATGTG
GTGGCGGACTTCAGGAAGGTTGGCTGGAATGTCCGTACTGCCTTACCCCACGCCAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(129-395)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

65.784

82.065

0.54

Multiple sequence alignment