Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACK8HE_RS03125 Genome accession   NZ_CP180192
Coordinates   649083..650063 (+) Length   326 a.a.
NCBI ID   WP_017692691.1    Uniprot ID   A0A2J0PYR2
Organism   Enterobacter hormaechei strain K_21_ENCL_294     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 644083..655063
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK8HE_RS03085 (ACK8HE_03085) - 644167..644493 (+) 327 WP_003862421.1 YggL family protein -
  ACK8HE_RS03090 (ACK8HE_03090) - 644550..645266 (+) 717 WP_015571842.1 DUF2884 domain-containing protein -
  ACK8HE_RS03095 (ACK8HE_03095) - 645350..645688 (-) 339 WP_015571841.1 endonuclease domain-containing protein -
  ACK8HE_RS03100 (ACK8HE_03100) hemW 645745..646893 (-) 1149 WP_045141926.1 radical SAM family heme chaperone HemW -
  ACK8HE_RS03105 (ACK8HE_03105) - 646886..647479 (-) 594 WP_015571839.1 XTP/dITP diphosphatase -
  ACK8HE_RS03110 (ACK8HE_03110) yggU 647483..647779 (-) 297 WP_003860023.1 DUF167 family protein YggU -
  ACK8HE_RS03115 (ACK8HE_03115) - 647776..648342 (-) 567 WP_015571838.1 YggT family protein -
  ACK8HE_RS03120 (ACK8HE_03120) - 648364..649065 (-) 702 WP_017692690.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACK8HE_RS03125 (ACK8HE_03125) pilT 649083..650063 (+) 981 WP_017692691.1 type IV pilus twitching motility protein PilT Machinery gene
  ACK8HE_RS03130 (ACK8HE_03130) ruvX 650073..650489 (-) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  ACK8HE_RS03135 (ACK8HE_03135) - 650489..651049 (-) 561 WP_006811924.1 YqgE/AlgH family protein -
  ACK8HE_RS03140 (ACK8HE_03140) gshB 651124..652071 (-) 948 WP_015571835.1 glutathione synthase -
  ACK8HE_RS03145 (ACK8HE_03145) rsmE 652103..652834 (-) 732 WP_017692692.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACK8HE_RS03150 (ACK8HE_03150) endA 652886..653593 (-) 708 WP_003860034.1 deoxyribonuclease I -
  ACK8HE_RS03155 (ACK8HE_03155) - 653688..654185 (-) 498 WP_017692693.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35682.70 Da        Isoelectric Point: 6.3047

>NTDB_id=1094383 ACK8HE_RS03125 WP_017692691.1 649083..650063(+) (pilT) [Enterobacter hormaechei strain K_21_ENCL_294]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVEALLKAWLNDEQQGAWWANGQVDFAVTLAGRQRLRG
SAFKHMLGVSIALRLLPLTCPQLSALGVPRAIPELLSNDNGLILVTGATGSGKSTTLAAMVDFLNHQTDGHILTLEDPVE
FMYQSERCLIQQREIGQHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLRQDVQGGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=1094383 ACK8HE_RS03125 WP_017692691.1 649083..650063(+) (pilT) [Enterobacter hormaechei strain K_21_ENCL_294]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGGTCAGGCCGTCTTGAACCTGCGCCGTTTCCGCCCCCGGATGTGGAGGCGTTATTAAAAGCGTGGCTCAACG
ACGAACAGCAGGGCGCATGGTGGGCAAACGGGCAAGTGGATTTTGCCGTTACCCTCGCAGGCCGTCAGCGGCTGCGCGGC
AGTGCGTTTAAGCATATGCTCGGCGTTTCGATTGCGCTGCGGCTGTTGCCGCTGACGTGCCCGCAGCTCTCTGCGCTAGG
TGTGCCGCGAGCGATCCCGGAACTACTGTCCAATGACAATGGCCTGATTCTGGTCACCGGCGCCACCGGCAGTGGGAAAT
CGACCACCCTGGCCGCTATGGTCGATTTCCTCAATCACCAGACGGACGGGCATATTCTGACCCTGGAAGATCCGGTGGAG
TTTATGTACCAGAGCGAACGTTGCCTGATCCAGCAGCGGGAGATAGGCCAGCACAGCCCGTCCTTTGCCGAGGCGCTGCG
CAGCGCGCTGCGTGAAGATCCGGATGTGATTCTGCTTGGTGAGCTGCGCGACAGCGAGACGATACGCCTGGCGCTGACGG
CGGCGGAAACCGGCCATCTGGTGTTAGCCACGCTGCATACGCGCGGCGCGGCGCAGGCGATCGAGCGCCTGGTCGACACC
TTTCCAGCGCAGGAGAAAGATCCGGTGCGTAATCAGCTGGCGGGAAGCCTGCGTGCGGTCCTGGCGCAGAAGCTACGTCA
GGATGTCCAGGGCGGGCGCGTGGCGCTGTATGAGCTACTGGTGAATACCTCGGCGGCGGCAAACCTGATCCGCGAAGGCA
AAACCTGGCAACTGCCGGGCATTATTCAAACGGGGCAGCAGGCGGGAATGCAGAACTTTGAGCAGAGTCTGGCGGAGCGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(108-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2J0PYR2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Legionella pneumophila strain Lp02

48.758

98.773

0.482
  pilT Legionella pneumophila strain ERS1305867

48.758

98.773

0.482
  pilT Pseudomonas stutzeri DSM 10701

48.012

100

0.482
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Acinetobacter baumannii strain A118

47.095

100

0.472
  pilT Pseudomonas aeruginosa PAK

47.095

100

0.472
  pilT Acinetobacter baumannii D1279779

47.095

100

0.472
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

47.5

98.16

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.344

99.08

0.429
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.387
  pilU Vibrio cholerae strain A1552

37.888

98.773

0.374

Multiple sequence alignment