Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACMGGG_RS22570 Genome accession   NZ_CP179908
Coordinates   4981287..4982288 (-) Length   333 a.a.
NCBI ID   WP_112899421.1    Uniprot ID   -
Organism   Pseudomonas sp. BNK-44-b     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4976287..4987288
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMGGG_RS22550 (ACMGGG_22550) argS 4977526..4979262 (+) 1737 WP_414154461.1 arginine--tRNA ligase -
  ACMGGG_RS22555 (ACMGGG_22555) - 4979264..4979962 (+) 699 WP_063541613.1 SPOR domain-containing protein -
  ACMGGG_RS22560 (ACMGGG_22560) - 4980010..4980429 (-) 420 WP_063541615.1 NINE protein -
  ACMGGG_RS22565 (ACMGGG_22565) - 4980598..4981230 (+) 633 WP_054891178.1 C40 family peptidase -
  ACMGGG_RS22570 (ACMGGG_22570) pilT 4981287..4982288 (-) 1002 WP_112899421.1 type IV pilus twitching motility protein PilT Machinery gene
  ACMGGG_RS22575 (ACMGGG_22575) - 4982354..4983040 (+) 687 WP_414154462.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACMGGG_RS22580 (ACMGGG_22580) proC 4983079..4983897 (+) 819 WP_414154463.1 pyrroline-5-carboxylate reductase -
  ACMGGG_RS22585 (ACMGGG_22585) - 4983908..4984498 (+) 591 WP_054891174.1 YggT family protein -
  ACMGGG_RS22590 (ACMGGG_22590) metX 4984587..4985726 (+) 1140 WP_054891173.1 homoserine O-succinyltransferase MetX -
  ACMGGG_RS22595 (ACMGGG_22595) metW 4985734..4986354 (+) 621 WP_063541621.1 methionine biosynthesis protein MetW -
  ACMGGG_RS22600 (ACMGGG_22600) - 4986379..4986813 (+) 435 WP_063541623.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 333 a.a.        Molecular weight: 36338.17 Da        Isoelectric Point: 7.0578

>NTDB_id=1093782 ACMGGG_RS22570 WP_112899421.1 4981287..4982288(-) (pilT) [Pseudomonas sp. BNK-44-b]
MDVTDLLARAVAAGASDLHLAAGEAPLWRLDGELQRLALPALQSEMIEKGLAPWLEADQRRLWPAAEEIDLALDLPGLGR
FRLNLYRQYRGLAASLRLIPAQVPTVEELDLMEVYRVIAQCRDGLVLVGGPTGSGKSSTLAALIAQLDRERSNHILTLED
PVEFVHRAERCLITQREIGRHSRDFAQGLRSALRQDPDVIMIGELRDLETIRLALRAAQTGHLVLATVHTRTAASSIDRL
VDVFAAEEKPLVRAMLAESLRLVVAQVLVRRVGGGRVAAREVLVATPAVRNLIREGRMAQLQSVMQTGTAQGMVTMEGAM
QALMKRGVIDGAG

Nucleotide


Download         Length: 1002 bp        

>NTDB_id=1093782 ACMGGG_RS22570 WP_112899421.1 4981287..4982288(-) (pilT) [Pseudomonas sp. BNK-44-b]
ATGGATGTGACCGACCTGTTGGCCCGGGCCGTGGCAGCGGGGGCTTCGGACCTGCACCTGGCTGCGGGCGAGGCGCCGCT
GTGGCGCCTGGATGGCGAATTGCAGCGCCTGGCTTTGCCTGCCTTGCAAAGCGAAATGATCGAGAAAGGCTTGGCGCCCT
GGCTCGAAGCTGATCAGCGGCGCCTGTGGCCGGCGGCTGAGGAAATCGATCTGGCCCTTGATCTGCCTGGGCTTGGCCGT
TTTCGTCTGAACCTGTACCGGCAGTATCGGGGGTTGGCCGCAAGCCTGCGACTGATACCTGCACAGGTGCCAACGGTCGA
AGAACTCGACCTCATGGAAGTGTATCGAGTCATTGCCCAGTGCCGTGATGGCCTGGTGCTGGTGGGTGGTCCGACCGGCA
GTGGCAAGTCCAGCACACTGGCTGCGTTGATCGCGCAGCTTGATCGTGAGCGCTCAAACCACATCCTCACCTTGGAAGAT
CCGGTCGAATTCGTGCACCGCGCCGAGCGCTGCCTGATCACTCAGCGCGAGATCGGTCGCCACAGTCGCGATTTTGCCCA
AGGGTTGCGCAGTGCCCTGCGCCAGGATCCGGATGTGATCATGATCGGGGAATTGCGTGACCTGGAGACCATTCGTCTGG
CGCTGCGAGCGGCGCAGACCGGGCATCTGGTGCTGGCTACGGTGCATACGCGAACAGCGGCGAGCAGCATCGATCGCCTG
GTGGACGTGTTCGCCGCCGAAGAGAAGCCGCTGGTGCGCGCCATGTTGGCCGAGTCGTTGCGTCTGGTGGTGGCGCAGGT
GCTGGTGCGTCGAGTAGGCGGCGGTCGTGTGGCGGCGCGGGAGGTATTGGTGGCGACGCCAGCGGTGCGCAACCTGATTC
GCGAAGGGCGGATGGCGCAGTTGCAGTCGGTGATGCAGACCGGCACGGCGCAGGGGATGGTGACGATGGAAGGGGCGATG
CAGGCTTTGATGAAGCGCGGAGTGATCGACGGGGCAGGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(109-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.964

99.399

0.517
  pilT Vibrio cholerae strain A1552

51.964

99.399

0.517
  pilT Acinetobacter baylyi ADP1

51.818

99.099

0.514
  pilT Acinetobacter baumannii D1279779

51.672

98.799

0.511
  pilT Acinetobacter nosocomialis M2

51.672

98.799

0.511
  pilT Acinetobacter baumannii strain A118

51.672

98.799

0.511
  pilT Pseudomonas stutzeri DSM 10701

51.064

98.799

0.505
  pilT Pseudomonas aeruginosa PAK

50.76

98.799

0.502
  pilT Neisseria meningitidis 8013

49.394

99.099

0.489
  pilT Neisseria gonorrhoeae MS11

49.091

99.099

0.486
  pilT Legionella pneumophila strain ERS1305867

48.328

98.799

0.477
  pilT Legionella pneumophila strain Lp02

48.328

98.799

0.477
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.152

99.099

0.447
  pilU Pseudomonas stutzeri DSM 10701

37.879

99.099

0.375
  pilU Vibrio cholerae strain A1552

36.923

97.598

0.36

Multiple sequence alignment