Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACMGF5_RS10650 Genome accession   NZ_CP179898
Coordinates   2314873..2315871 (+) Length   332 a.a.
NCBI ID   WP_251962086.1    Uniprot ID   -
Organism   Pseudomonas sp. BNK-15     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2309873..2320871
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMGF5_RS10620 (ACMGF5_10620) - 2310350..2310784 (-) 435 WP_046787038.1 DUF4426 domain-containing protein -
  ACMGF5_RS10625 (ACMGF5_10625) metW 2310807..2311427 (-) 621 WP_012312310.1 methionine biosynthesis protein MetW -
  ACMGF5_RS10630 (ACMGF5_10630) metX 2311435..2312574 (-) 1140 WP_046787039.1 homoserine O-succinyltransferase MetX -
  ACMGF5_RS10635 (ACMGF5_10635) - 2312663..2313253 (-) 591 WP_046787040.1 YggT family protein -
  ACMGF5_RS10640 (ACMGF5_10640) proC 2313264..2314082 (-) 819 WP_196997346.1 pyrroline-5-carboxylate reductase -
  ACMGF5_RS10645 (ACMGF5_10645) - 2314121..2314807 (-) 687 WP_046787042.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACMGF5_RS10650 (ACMGF5_10650) pilT 2314873..2315871 (+) 999 WP_251962086.1 type IV pilus twitching motility protein PilT Machinery gene
  ACMGF5_RS10655 (ACMGF5_10655) - 2315908..2316525 (-) 618 WP_046787044.1 C40 family peptidase -
  ACMGF5_RS10660 (ACMGF5_10660) - 2316702..2317121 (+) 420 WP_039602507.1 NINE protein -
  ACMGF5_RS10665 (ACMGF5_10665) - 2317166..2317864 (-) 699 WP_046787045.1 SPOR domain-containing protein -
  ACMGF5_RS10670 (ACMGF5_10670) argS 2317866..2319602 (-) 1737 WP_115273369.1 arginine--tRNA ligase -

Sequence

Protein


Download         Length: 332 a.a.        Molecular weight: 36189.81 Da        Isoelectric Point: 7.4089

>NTDB_id=1093550 ACMGF5_RS10650 WP_251962086.1 2314873..2315871(+) (pilT) [Pseudomonas sp. BNK-15]
MDVTDLLARAGDAGASDLHLAAGEIPMLRVDGELRRMALPNLTPAALTDALAPLLDECRRQQWAQGDEQDLALELPSLGR
FRLNLFRQLNGPAATFRLIPKRIATLDELDLEDVFRSVAQWTDGLILLGGPTGSGKSSTLATLIDQLNRQRSLHIITLED
PVEVIHNSQRSLINQREIGRHCRDFSQGLRSALRQDPDVIVIGELRDLETIRLALRAAETGHLVLATVHTRSAAGSVDRL
VEVFAAEEKPLVRAMLAESLRMVVAQVLVKCVRGGRVAAREVLVGTPAVRNLIREGRMAQLNSVMQAGAAQGMRTMEGAL
QRLKTQGLIGAV

Nucleotide


Download         Length: 999 bp        

>NTDB_id=1093550 ACMGF5_RS10650 WP_251962086.1 2314873..2315871(+) (pilT) [Pseudomonas sp. BNK-15]
ATGGACGTGACTGACCTGTTGGCCCGGGCTGGCGATGCAGGGGCGAGTGACCTGCACCTGGCCGCGGGCGAGATACCGAT
GCTGCGCGTGGATGGTGAGCTGCGGCGCATGGCGCTACCGAACTTGACGCCAGCGGCGTTGACCGATGCCCTGGCGCCGC
TGCTCGATGAGTGCCGGCGCCAGCAGTGGGCGCAAGGTGATGAGCAGGACCTGGCACTGGAGCTGCCCTCGCTCGGGCGC
TTTCGCCTGAACCTGTTCCGTCAGTTGAATGGCCCGGCGGCCACCTTTCGCTTGATCCCAAAGCGGATTGCGACGCTCGA
CGAGCTTGACCTGGAAGATGTGTTTCGGTCTGTTGCACAGTGGACTGACGGCCTGATTCTGCTCGGCGGGCCTACCGGCA
GTGGAAAATCCAGCACCTTGGCGACGCTGATCGATCAGCTGAACCGGCAGCGGTCCTTGCACATCATCACCCTCGAGGAC
CCTGTCGAAGTTATCCACAACAGCCAGCGAAGCCTGATCAATCAGCGAGAAATCGGTCGGCATTGCCGCGACTTTTCCCA
AGGGCTGCGCAGCGCGCTGCGTCAGGACCCGGATGTGATCGTCATAGGCGAATTGCGCGACCTGGAGACGATTCGCCTGG
CGTTGCGGGCTGCGGAGACCGGGCACCTGGTGCTGGCAACGGTGCATACGCGCTCGGCTGCTGGCAGTGTCGACCGGCTG
GTGGAGGTGTTTGCCGCCGAGGAGAAACCGCTGGTGCGAGCGATGTTGGCGGAGTCGCTGCGTATGGTTGTGGCGCAGGT
GCTGGTCAAGTGTGTGCGGGGTGGGCGGGTGGCTGCCCGGGAGGTGCTGGTGGGAACGCCTGCGGTGCGTAACCTGATCC
GCGAGGGGCGTATGGCGCAATTGAATTCGGTGATGCAGGCGGGTGCTGCGCAGGGCATGCGGACGATGGAGGGGGCGTTG
CAGCGATTGAAGACCCAAGGGTTGATCGGGGCCGTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

51.368

99.096

0.509
  pilT Acinetobacter baylyi ADP1

50.909

99.398

0.506
  pilT Neisseria meningitidis 8013

50.755

99.699

0.506
  pilT Pseudomonas stutzeri DSM 10701

50.76

99.096

0.503
  pilT Neisseria gonorrhoeae MS11

50.453

99.699

0.503
  pilT Acinetobacter nosocomialis M2

50.456

99.096

0.5
  pilT Acinetobacter baumannii D1279779

50.456

99.096

0.5
  pilT Acinetobacter baumannii strain A118

50.456

99.096

0.5
  pilT Vibrio cholerae strain A1552

49.245

99.699

0.491
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.245

99.699

0.491
  pilT Legionella pneumophila strain Lp02

48.485

99.398

0.482
  pilT Legionella pneumophila strain ERS1305867

48.485

99.398

0.482
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.939

99.398

0.437
  pilU Pseudomonas stutzeri DSM 10701

37.576

99.398

0.373
  pilU Acinetobacter baylyi ADP1

36.364

99.398

0.361

Multiple sequence alignment